Antibacterial Activity of DMSO Extracts of Selected Plants Against Antibiotic Resistant Clinical Isolates

Year 2019, , 576 - 584, 31.08.2019

Ayşegül Saral , Uğur Kardil Azer Özad Düzgün

https://doi.org/10.18185/erzifbed.451871

Cited By: 1

Abstract

In this study, we aimed to find out new herbal materials
that are able to inhibit the growth of the P.
aeruginosa and E.coli clinical isolates
that has antibiotic resistance. Clinical isolates used in this research are E. coli (n=1) and P.aeruginosa (n=1). Antibiotic susceptibility profiles of E. coli and P. aeruginosa were determined using e-test. Plants were collected
in Trabzon region of Turkey are Calendula officinalis, Hypericum perforatum and Glycyrrhiza glabra. DMSO were used as
solvent and solid-liquid extraction was employed. Micro-dilution method was
preferred fo the determination of the minimum inhibitory concentration (MIC). MIC
results were obtained through observation of turbidities. According to E-test
results, while P. aeruginosa was
resistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime, E. coli was resistant to piperacillin,
cefotaxime and ceftazidime. DMSO extract of Calendula
officinalis showed very strong activity against PA1 with the best MIC (5
mg/mL).  DMSO extract of three plant had
lower MIC values (5-10 mg/ml) for EC1 and PA1 than ampicillin. In future
studies antibacterial activity of different solvents extracts of these plants
and other plants against antibiotic resistant clinical isolates will be
examined. Natural products from plants are promising in fighting with
antibiotic-resistant bacteria.

Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi

Abstract

Bu çalışmada, antibiyotik dirençli P. aeruginosa ve E.coli
klinik izolatlarının büyümesini engelleyebilecek yeni bitkisel materyallerin
keşfi amaçlanmıştır. Bu çalışmada kullanılan klinik izolatlar E. coli (n = 1) ve P.aeruginosa'dır (n = 1). E.
coli ve P. aeruginosa'nın
antibiyotik duyarlılık profilleri e-test kullanılarak belirlenmiştir. Trabzon
yöresinden Calendula officinalis,
Hypericum perforatum and Glycyrrhiza
glabra bitkileri toplanmıştır. Çözücü olarak DMSO kullanılmış ve katı-sıvı
ekstraksiyon yapılmıştır. Minimum inhibitör konsantrasyonunun (MİK)
belirlenmesi için mikro dilüsyon yöntemi tercih edilmiştir. MİK sonuçları
türbiditelerin gözlemlenmesiyle elde edilmiştir. E-test sonuçlarına göre, P. aeruginosa piperasilin,
piperasilin/tazobaktam, meropenem ve seftazidime dirençli iken, E. coli piperasilin, sefotaksim ve
seftazidime dirençliydi. Calendula
officinalis'in DMSO özütü, en iyi MIC (5 mg / mL) ile PA1'e karşı çok güçlü
aktivite göstermiştir. Üç bitkinin DMSO özütü, EC1 ve PA1 için ampisilin
değerinden daha düşük MIC değerlerine (5-10 mg / ml) sahip olduğu tespit
edilmiştir. Gelecekteki çalışmalarda, bu bitkilerin ve diğer bitkilerin
antibiyotik dirençli klinik izolatlara karşı farklı çözücülerle hazırlanan
özütlerinin antibakteriyel aktiviteleri incelenecektir. Bitkilerden elde edilen
doğal ürünler, antibiyotiğe dirençli bakterilerle savaşırken ümit vericidir.

Keywords

Antibiotic Resistance, Pseudomonas aeruginosa, Escherichia coli, Minimum Inhibitory Concentration, Plant Extract

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