Research Article
BibTex RIS Cite

Antibacterial Activity of DMSO Extracts of Selected Plants Against Antibiotic Resistant Clinical Isolates

Year 2019, , 576 - 584, 31.08.2019
https://doi.org/10.18185/erzifbed.451871

Abstract

In this study, we aimed to find out new herbal materials
that are able to inhibit the growth of the P.
aeruginosa
and E.coli clinical isolates
that has antibiotic resistance. Clinical isolates used in this research are E. coli (n=1) and P.aeruginosa (n=1). Antibiotic susceptibility profiles of E. coli and P. aeruginosa were determined using e-test. Plants were collected
in Trabzon region of Turkey are Calendula officinalis, Hypericum perforatum and Glycyrrhiza glabra. DMSO were used as
solvent and solid-liquid extraction was employed. Micro-dilution method was
preferred fo the determination of the minimum inhibitory concentration (MIC). MIC
results were obtained through observation of turbidities. According to E-test
results, while P. aeruginosa was
resistant to piperacillin, piperacillin/tazobactam, meropenem and ceftazidime, E. coli was resistant to piperacillin,
cefotaxime and ceftazidime. DMSO extract of Calendula
officinalis
showed very strong activity against PA1 with the best MIC (5
mg/mL).  DMSO extract of three plant had
lower MIC values (5-10 mg/ml) for EC1 and PA1 than ampicillin. In future
studies antibacterial activity of different solvents extracts of these plants
and other plants against antibiotic resistant clinical isolates will be
examined. Natural products from plants are promising in fighting with
antibiotic-resistant bacteria.

References

  • Referans1 Abbas, A., Zubair, M., Rasool, N., Rizwan, K. (2015). “Antimicrobial Potential of Glycyrrhiza glabra”, Journal of Drug Design and Medicinal Chemistry, 1(2), 17-20.
  • Referans2 Akova, M. (2016). Epidemiology of antimicrobial resistance in bloodstream infections. Virulence, 7(3), 252–266.
  • Referans3 Andersen, NF., Møller, J., Peterslund, NA. (2005). “Piperacillin-resistant Escherichia coli bacteraemia: Relation to empiric therapy and clinical outcome”, Scand J Infect Dis, 37(2),90-5.
  • Referans4 Cantas, L., Shah, SQ., Cavaco, LM., Manaia, CM., Walsh, F., Popowska, M., Garelick, H., Bürgmann, H., Sørum, H. (2013). “A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota”, Front Microbiol, 14;4:96.
  • Referans5 Chandurkar, P., Murab, T., Ahakey, N., Tripathi, N., Choudhary, A. (2015) “Antimicrobial activity of aqueous, acetone and methanol extracts of Calendulaofficinalis L. (Marigold) flower”, Int J Pure App Biosci, 3 (2), 386-388.
  • Referans6 Dhama, K., Tiwari R., Chakraborty S., Saminathan M., Kumar A., Karthik K., Wani MY., Amarpal, Singh SV. and Rahal A. (2014). “Evidence Based Antibacterial Potentials of Medicinal Plants and Herbs Countering Bacterial Pathogens Especially in the Era of Emerging Drug Resistance: An Integrated Update”, International Journal of Pharmacology, 10, 1-43.
  • Referans7 Direkel S, Uzunoglu E, Uzalp C, Findik E, Tontak S, et al. (2017). “Determination of Piperacillin/Tazobactam and Ticarcillin/Clavulanate Susceptibilities in Pseudomonas aeruginosa Isolates in Hospitalised Patients by E-test Gradient Method and Comparison of Results with Disk Diffusion Tests”, Clin Microbiol 6:273. doi:10.4172/2327-5073.1000273.
  • Referans8 EUCAST. “The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 8.1, 2018”, http://www.eucast.org, 05.06.18.
  • Referans9 Hunter, PA., Dawson, S., French, GL., Goossens, H., Hawkey, P.M., Kuijper, EJ., Nathwani, D., Taylor, DJ., Teale, CJ., Warren, RE., Wilcox, MH., Woodford, N., Wulf, MW., Piddock, LJ. (2010). “Antimicrobial-resistant pathogens in animals and man: prescribing, practices and policies”, J Antimicrob Chemother, 65 (1),i3-17.
  • Referans10 Karahan, F., Avsar, C., Ozyigit, II., Berber I. (2016). “Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats”, Biotechnology & Biotechnological Equipment, 30 (4), 797-804.
  • Referans11 Kim, A., Sutherland, CA., Kuti, JL., Nicolau, DP. (2007). “Optimal dosing of piperacillin-tazobactam for the treatment of Pseudomonas aeruginosa infections: prolonged or continuous infusion?” Pharmacotherapy, 27(11),1490-7.
  • Referans12 Kos, VN., McLaughlin, RE., Gardner, HA. (2016). “Elucidation of Mechanisms of Ceftazidime Resistance among Clinical Isolates of Pseudomonas aeruginosa by Using Genomic Data”, Antimicrobial Agents and Chemotherapy, 60(6), 3856–3861.
  • Referans13 Lodise, TP., Jr, Lomaestro, B., Drusano, GL. (2007). “Piperacillin-Tazobactam for Pseudomonas aeruginosa Infection: Clinical Implications of an Extended-Infusion Dosing Strategy”, Clin Infect Dis, 44(3),357-63.
  • Referans14 Mirsalehian, A., Kalantar-Neyestanaki, D., Taherikalani, M., Jabalameli, F., Emaneini, M. (2017). “Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran”, J Epidemiol Glob Health, 7(3),155-159.
  • Referans15 Nitalikar, MM., Munde, KC., Dhore, BV., Shikalgar, SN. (2010). “Studies of Antibacterial Activities of Glycyrrhiza glabra Root Extract”, International Journal of PharmTech Research, 2 (1), 899-901.
  • Referans16 Oskay, M., Oskay, D., Kalyoncu, F. (2009). “Activity of Some Plant Extracts Against Multi-Drug Resistant Human Pathogens”, Iranian Journal of Pharmaceutical Research, 8 (4), 293-300.
  • Rerferasn17 Reichling, J., Weseler, A., Saller, RA. 2001. “Current Review of the Antimicrobial Activity of Hypericum perforatum L”, Pharmacopsychiatry, 34 (1),116-8.
  • Referans18 Rostami, S., Farajzadeh, Sheikh, A., Shoja, S., Farahani, A., Tabatabaiefar, MA., Jolodar, A., Sheikhi, R. (2018). “Investigating of four main carbapenem-resistance mechanisms in high-level carbapenem resistant Pseudomonas aeruginosa isolated from burn patients”, J Chin Med Assoc, 81(2),127-132.
  • Referans19 Shah Pratibha, J., Williamson Manita, T. (2015). “Antibacterial and Synergistic activity of Calendula officinalis Methanolic Petal Extract on Klebsiella pneumoniae Co-producing ESBL and AmpC Beta Lactamase”, Int J Curr Microbiol App Sci ,4(4), 107-117.
  • Referasn20 Srivastava, J., Chandra, H., Nautiyal, AR., Kalra, SJS. (2014). "Antimicrobial resistance (AMR) and plant-derived antimicrobials (PDAms) as an alternative drug line to control infections”, 3 Biotech, 4(5), 451–460.
  • Referans21 Süntar, I., Oyardı, O., Akkol, EK., Ozçelik, B. (2016). “Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation”, Pharm Biol,54(6),1065-70.
  • Referans22 Tavío, MM., Aquili, VD., Vila, J., Poveda, JB. (2014). “Resistance to ceftazidime in Escherichia coli associated with AcrR, MarR and PBP3 mutations and overexpression of sdiA”, J Med Microbiol, 63(1),56-65.
  • Referans23 Vila, J., Sáez-López, E., Johnson, JR., Römling, U., Dobrindt, U., Cantón, R., Giske, CG., Naas, T., Carattoli, A., Martínez-Medina, M., Bosch, J., Retamar, P., Rodríguez-Baño, J., Baquero, F., Soto, SM. (2016). “Escherichia coli: an old friend with new tidings”,FEMS Microbiol Rev, 40(4),437-463.
  • Referans24 Wikaningtyas, P., Sukandar, EY. (2016). “The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens”, Asian Pacific Journal of Tropical Biomedicine, 6 (1),16-19.
  • Referans25 Yuan, XY., Yu, DY., Qu, XH., Xiao, XQ., Bi, B., Sun, SB., Chang, AY., Zhang, QB. (2016). “Increased resistance rate to ceftazidime among blood culture isolates of ESBL-producing Escherichia coli in a university-affiliated hospital of China”, J Antibiot (Tokyo), 69(3),169-72.

Seçilen Bitkilerin DMSO Özütlerinin Antibiyotik Dirençli Klinik İzolatlara Karşı Antibakteriyel Aktivitesi

Year 2019, , 576 - 584, 31.08.2019
https://doi.org/10.18185/erzifbed.451871

Abstract

Bu çalışmada, antibiyotik dirençli P. aeruginosa ve E.coli
klinik izolatlarının büyümesini engelleyebilecek yeni bitkisel materyallerin
keşfi amaçlanmıştır. Bu çalışmada kullanılan klinik izolatlar E. coli (n = 1) ve P.aeruginosa'dır (n = 1). E.
coli
ve P. aeruginosa'nın
antibiyotik duyarlılık profilleri e-test kullanılarak belirlenmiştir. Trabzon
yöresinden Calendula officinalis,
Hypericum perforatum
and Glycyrrhiza
glabra
bitkileri toplanmıştır. Çözücü olarak DMSO kullanılmış ve katı-sıvı
ekstraksiyon yapılmıştır. Minimum inhibitör konsantrasyonunun (MİK)
belirlenmesi için mikro dilüsyon yöntemi tercih edilmiştir. MİK sonuçları
türbiditelerin gözlemlenmesiyle elde edilmiştir. E-test sonuçlarına göre, P. aeruginosa piperasilin,
piperasilin/tazobaktam, meropenem ve seftazidime dirençli iken, E. coli piperasilin, sefotaksim ve
seftazidime dirençliydi. Calendula
officinalis
'in DMSO özütü, en iyi MIC (5 mg / mL) ile PA1'e karşı çok güçlü
aktivite göstermiştir. Üç bitkinin DMSO özütü, EC1 ve PA1 için ampisilin
değerinden daha düşük MIC değerlerine (5-10 mg / ml) sahip olduğu tespit
edilmiştir. Gelecekteki çalışmalarda, bu bitkilerin ve diğer bitkilerin
antibiyotik dirençli klinik izolatlara karşı farklı çözücülerle hazırlanan
özütlerinin antibakteriyel aktiviteleri incelenecektir. Bitkilerden elde edilen
doğal ürünler, antibiyotiğe dirençli bakterilerle savaşırken ümit vericidir.

References

  • Referans1 Abbas, A., Zubair, M., Rasool, N., Rizwan, K. (2015). “Antimicrobial Potential of Glycyrrhiza glabra”, Journal of Drug Design and Medicinal Chemistry, 1(2), 17-20.
  • Referans2 Akova, M. (2016). Epidemiology of antimicrobial resistance in bloodstream infections. Virulence, 7(3), 252–266.
  • Referans3 Andersen, NF., Møller, J., Peterslund, NA. (2005). “Piperacillin-resistant Escherichia coli bacteraemia: Relation to empiric therapy and clinical outcome”, Scand J Infect Dis, 37(2),90-5.
  • Referans4 Cantas, L., Shah, SQ., Cavaco, LM., Manaia, CM., Walsh, F., Popowska, M., Garelick, H., Bürgmann, H., Sørum, H. (2013). “A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota”, Front Microbiol, 14;4:96.
  • Referans5 Chandurkar, P., Murab, T., Ahakey, N., Tripathi, N., Choudhary, A. (2015) “Antimicrobial activity of aqueous, acetone and methanol extracts of Calendulaofficinalis L. (Marigold) flower”, Int J Pure App Biosci, 3 (2), 386-388.
  • Referans6 Dhama, K., Tiwari R., Chakraborty S., Saminathan M., Kumar A., Karthik K., Wani MY., Amarpal, Singh SV. and Rahal A. (2014). “Evidence Based Antibacterial Potentials of Medicinal Plants and Herbs Countering Bacterial Pathogens Especially in the Era of Emerging Drug Resistance: An Integrated Update”, International Journal of Pharmacology, 10, 1-43.
  • Referans7 Direkel S, Uzunoglu E, Uzalp C, Findik E, Tontak S, et al. (2017). “Determination of Piperacillin/Tazobactam and Ticarcillin/Clavulanate Susceptibilities in Pseudomonas aeruginosa Isolates in Hospitalised Patients by E-test Gradient Method and Comparison of Results with Disk Diffusion Tests”, Clin Microbiol 6:273. doi:10.4172/2327-5073.1000273.
  • Referans8 EUCAST. “The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 8.1, 2018”, http://www.eucast.org, 05.06.18.
  • Referans9 Hunter, PA., Dawson, S., French, GL., Goossens, H., Hawkey, P.M., Kuijper, EJ., Nathwani, D., Taylor, DJ., Teale, CJ., Warren, RE., Wilcox, MH., Woodford, N., Wulf, MW., Piddock, LJ. (2010). “Antimicrobial-resistant pathogens in animals and man: prescribing, practices and policies”, J Antimicrob Chemother, 65 (1),i3-17.
  • Referans10 Karahan, F., Avsar, C., Ozyigit, II., Berber I. (2016). “Antimicrobial and antioxidant activities of medicinal plant Glycyrrhiza glabra var. glandulifera from different habitats”, Biotechnology & Biotechnological Equipment, 30 (4), 797-804.
  • Referans11 Kim, A., Sutherland, CA., Kuti, JL., Nicolau, DP. (2007). “Optimal dosing of piperacillin-tazobactam for the treatment of Pseudomonas aeruginosa infections: prolonged or continuous infusion?” Pharmacotherapy, 27(11),1490-7.
  • Referans12 Kos, VN., McLaughlin, RE., Gardner, HA. (2016). “Elucidation of Mechanisms of Ceftazidime Resistance among Clinical Isolates of Pseudomonas aeruginosa by Using Genomic Data”, Antimicrobial Agents and Chemotherapy, 60(6), 3856–3861.
  • Referans13 Lodise, TP., Jr, Lomaestro, B., Drusano, GL. (2007). “Piperacillin-Tazobactam for Pseudomonas aeruginosa Infection: Clinical Implications of an Extended-Infusion Dosing Strategy”, Clin Infect Dis, 44(3),357-63.
  • Referans14 Mirsalehian, A., Kalantar-Neyestanaki, D., Taherikalani, M., Jabalameli, F., Emaneini, M. (2017). “Determination of carbapenem resistance mechanism in clinical isolates of Pseudomonas aeruginosa isolated from burn patients, in Tehran, Iran”, J Epidemiol Glob Health, 7(3),155-159.
  • Referans15 Nitalikar, MM., Munde, KC., Dhore, BV., Shikalgar, SN. (2010). “Studies of Antibacterial Activities of Glycyrrhiza glabra Root Extract”, International Journal of PharmTech Research, 2 (1), 899-901.
  • Referans16 Oskay, M., Oskay, D., Kalyoncu, F. (2009). “Activity of Some Plant Extracts Against Multi-Drug Resistant Human Pathogens”, Iranian Journal of Pharmaceutical Research, 8 (4), 293-300.
  • Rerferasn17 Reichling, J., Weseler, A., Saller, RA. 2001. “Current Review of the Antimicrobial Activity of Hypericum perforatum L”, Pharmacopsychiatry, 34 (1),116-8.
  • Referans18 Rostami, S., Farajzadeh, Sheikh, A., Shoja, S., Farahani, A., Tabatabaiefar, MA., Jolodar, A., Sheikhi, R. (2018). “Investigating of four main carbapenem-resistance mechanisms in high-level carbapenem resistant Pseudomonas aeruginosa isolated from burn patients”, J Chin Med Assoc, 81(2),127-132.
  • Referans19 Shah Pratibha, J., Williamson Manita, T. (2015). “Antibacterial and Synergistic activity of Calendula officinalis Methanolic Petal Extract on Klebsiella pneumoniae Co-producing ESBL and AmpC Beta Lactamase”, Int J Curr Microbiol App Sci ,4(4), 107-117.
  • Referasn20 Srivastava, J., Chandra, H., Nautiyal, AR., Kalra, SJS. (2014). "Antimicrobial resistance (AMR) and plant-derived antimicrobials (PDAms) as an alternative drug line to control infections”, 3 Biotech, 4(5), 451–460.
  • Referans21 Süntar, I., Oyardı, O., Akkol, EK., Ozçelik, B. (2016). “Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation”, Pharm Biol,54(6),1065-70.
  • Referans22 Tavío, MM., Aquili, VD., Vila, J., Poveda, JB. (2014). “Resistance to ceftazidime in Escherichia coli associated with AcrR, MarR and PBP3 mutations and overexpression of sdiA”, J Med Microbiol, 63(1),56-65.
  • Referans23 Vila, J., Sáez-López, E., Johnson, JR., Römling, U., Dobrindt, U., Cantón, R., Giske, CG., Naas, T., Carattoli, A., Martínez-Medina, M., Bosch, J., Retamar, P., Rodríguez-Baño, J., Baquero, F., Soto, SM. (2016). “Escherichia coli: an old friend with new tidings”,FEMS Microbiol Rev, 40(4),437-463.
  • Referans24 Wikaningtyas, P., Sukandar, EY. (2016). “The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens”, Asian Pacific Journal of Tropical Biomedicine, 6 (1),16-19.
  • Referans25 Yuan, XY., Yu, DY., Qu, XH., Xiao, XQ., Bi, B., Sun, SB., Chang, AY., Zhang, QB. (2016). “Increased resistance rate to ceftazidime among blood culture isolates of ESBL-producing Escherichia coli in a university-affiliated hospital of China”, J Antibiot (Tokyo), 69(3),169-72.
There are 25 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Ayşegül Saral

Uğur Kardil This is me

Azer Özad Düzgün

Publication Date August 31, 2019
Published in Issue Year 2019

Cite

APA Saral, A., Kardil, U., & Özad Düzgün, A. (2019). Antibacterial Activity of DMSO Extracts of Selected Plants Against Antibiotic Resistant Clinical Isolates. Erzincan University Journal of Science and Technology, 12(2), 576-584. https://doi.org/10.18185/erzifbed.451871