Research Article
BibTex RIS Cite

Şakok Armudunun (Pyrus elaeagnifila Pallas) Antioksidan, Antimikrobiyal ve Mutajenik Özelliklerinin İncelenmesi

Year 2019, , 447 - 456, 24.03.2019
https://doi.org/10.18185/erzifbed.489779

Abstract

Şakok
armudu (Pyrus elaeagnifila Pallas) doğal olarak yetişen yabani bir armut
türüdür. Hasattan sonra olgunlaşır ve yenebilir. Bu çalışma Elazığ’da yetişen
şakok armudunun toplam fenolik madde, toplam flavanoid madde, toplam askorbik
asit, glutatyon (GSH) ve süperoksid dismutaz (SOD) içeriklerinin; antioksidan
aktivite, antimikrobiyal aktivite ve mutajenik aktivitesinin belirlenmesini
kapsamaktadır. Elde edilen verilere göre örneklerin toplam fenolik madde
içeriği 174.2 mg GAE (gallik asit eşdeğeri)/100g ekstrakt, toplam flavanoid
madde içeriği 44.9 mg RE (rutin eşdeğeri)/100g ekstrakt, toplam askorbik asit
içeriği 14.5 mg AE (askorbik asit eşdeğeri)/100g ekstrakt, Demir iyonu
indirgeyici antioksidan güç (FRAP) değeri 515.6 µmol Fe (II)/g ekstrakt, 2,2′-Azino-bis
3-etil benzotiazolin-6-sulfonik asit (ABTS) süpürme aktivitesi % 53.5,
1,1-difenil-2-pikrilhidrazil (DPPH) süpürme aktivitesi  % 48.2, GSH içeriği 3.98 µM ve SOD içeriği
6.88 U/ml olarak tespit edilmiştir. Meyve ekstraktının test bakterilerine karşı
farklı oranlarda antimikrobiyal aktivite gösterdiği belirlenmiştir. Meyve
ekstraktının ön denemeler sonucunda belirlenen altı farklı konsantrasyonunun
(10, 20, 40, 60, 80 and 100 µL/plak) 
Salmonella typhimurium TA 98 ve TA 100 şuşlarına karşı mutajenik aktivite
gösterip göstermediği test edilmiştir. Denenen dozların hiçbirinde TA 98 ve TA
100 şuşları üzerinde istatistiksel olarak önemli bir mutajenik etki
görülmemiştir. Bu çalışma şakok armudunun önemli biyoaktif bileşenlere,
antioksidan ve antimikrobiyal aktiviteye sahip olduğunu ortaya çıkarmıştır.

References

  • Abacı, Z.T., Sevindik, E., Ayvaz, M. 2016. Comparative study of bioactive components in pear genotypes from Ardahan/Turkey. Biotechnology & Biotechnological Equipment, 30, 1, 36-43.
  • Anonim 2013. Ahlat bitkisi nedir, nasıl kullanılır? (http://www.hastalıktedavi.net), Son erişim tarihi: 19.11.2018
  • Anonim 2017. https://www.1organik.com/ahlat-agaci-ahlat-armudu.html, Son erişim tarihi: 19.11.2018
  • AOAC, 1990. Official methods of analysis of the Association of Official Analytical Chemists, 15th ed., Arlington VA, 1058-1059.
  • Aslan, G., Yazıcı, K. 2017. Doğanın Bize Sunduğu Görsel Şölen Yabani Meyveler I. Türktob, 21, 60-64.
  • Bakhshi, D., Arakawa, O. 2006. Effects of UV-b irradiation on phenolic compound accumulation and antioxidant activity in ‘Jonathan’ apple influenced by bagging, temperature and maturation. Journal of Food, Agriculture & Environment, 4, 1, 75-79.
  • Baltas, N. 2017. Investigation of a wild pear species (Pyrus elaeagnifolia subsp. Elaeagnifolia Pallas) from Antalya, Turkey: polyphenol oxidase properties and anti-xanthine oxidase, anti-urease, and antioxidant activity. International Journal of Food Properties, 20, 3, 585-595.
  • Benzie, I.F.F, Strain, J.J. 1996. The ferric reducing Ability of plasma (FRAB) as a measure of Antioxidant power: The FRAB assay. Analytical Biochemistry, 239, 70-76.
  • Collins, C.M., Lyne, P.M., Grange, J.M., 1989. Antimicrobial sensitivity and assay tests. Collins and Lyne’s Microbiological Methods. Butterworths, London Coşkun, T., 2005. Fonksiyonel besinlerin sağlığımız üzerine etkileri. Çocuk Sağlığı ve Hastalıkları Dergisi, 48, 69-84.
  • Çakılcıoğlu, U., Şengün, M.T., Türkoğlu, D. 2010. An ethnobotanical survey of medicinal plants of Yazıkonak and Yurtbaşı districts of Elazığ province, Turkey. Journal of Medicinal Plants Research, 4, 7, 567-572.
  • Heimler, D. Vignolini, P. Dini, M.G. Vincieri, F.F. Pomani, A. 2006. Antiradical activity and polyphenol composition of local Brassicaiceae adible varieties. Food Chemistry, 99, 464-469.
  • Erbil, N., Murathan, Z. T., Arslan, M., Ilcim, A., Sayin, B. 2018. Antimicrobial, Antioxidant, and Antimutagenic Activities of Five Turkish Pear Cultivars. Erwerbs-Obstbau, 60, 3, 203-209.
  • Güdücü, F. 2014. Pyrus elaeagrifolia bitkisi ekstrelerinin fenolik madde içerikleri, DPPH radikali giderme aktiviteleri ve in vitro antimikrobiyal etkilerinin belirlenmesi. Master's thesis, Trakya Üniversitesi.
  • Justesen, U., Knuthsen, P., Leth, T. 1998. Quantitative analysis of flavonols, flavones, and flavonones in fruits, vegetables and beverages by high-performance liquid chromatography with photodiode array and massspectrometric detection. Journal of Chromatography A, 799, 101-110.
  • Keçeci, L. D. 2017. Hakkari yöresi üstün nitelikli ahlat (Pyrus elaegrifolia L.) genotiplerinin bazı özelliklerinin belirlenmesi. Master's thesis, Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü.
  • Maron, D., Ames, B. 1983. Revised Methods for the Salmonella Mutagenicity Test. Mutation Research, 113, 173-215.
  • Mbaebie, B.O., Edeoga, H.O., Ajelayan, A.J. 2012. Phytochemical analysis and antioxidant activities of aqueous bark extract of Schoba lahtolic JACQ. Asian Pacific Journal of Tropical Biomedicine, 2, 118-124.
  • Naczk, M., Shahidi, F. 2006 Phenolics in cereals, fruits and vegetables: Occurence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis 41, 1523-1542.
  • Nizamoğlu, N.M., Nas, S. 2010. Meyve ve Sebzelerde Bulunan Fenolik Bileşikler; Yapıları ve Önemleri. Gıda Teknolojileri Elektronik Dergisi, 5, 1, 20-35. Özbek, S. 1985. Genel Meyvecilik. Çukurova Üniversitesi Ziraat Fak. Yayınları Ders Kitabı, 386.
  • Parejo, L., Codina, C., Petrakis, C., Kefalas, P. 2000. Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH center dot (2,2-diphenyl-1- picrylhydrazyl) free radical assay. Journal of Pharmacological and Toxicological Methods, 44, 507-512.
  • Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, J., Luyck, M., Cazin, M., Cazin, J.C., Bailleul, F., and Trotin, F. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72, 35-40.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 9/10, 1231-1237.
  • Sanchez-Moreno C, Plaza L, De Ancos B, et al. 2003. Quantitative bioactive compounds assessment and their relative contribution to the antioxidant capacity of commercial orange juices. J Sci Food Agr. 83, 430-439.
  • Sedlak, J., Lindsay, R.H. 1968. Estimation of Total Protein-Bound, and Nonprotein Sulfhydryl Groups in Tissue with Ellman's Reagent. Analytical Biochemistry, 25, 1, 192-205.
  • Smatana, L., Kytka, J., Kadarova, S. 1988. Results of Breeding and Groving Minor Fruit Species in Czechoslovakia. Acta Horticulture, 224, 83-87.
  • Smirnoff N. 1996 The function and metabolism of ascorbic acid in plants. Ann Bot. 78, 661-669.
  • Smirnova, G.V., Oktyabrsky, O.N. 2005. Glutathione in Bacteria. Biochemistry (Moscow), 70 11, 1199-1211.
  • Spanos, G.A., Wrolstad, R.E. 1992. Phenolic of apple, pear and white grape juices and their changes with processing and storage. Journal of Agriculture and Food Chemistry, 40, 9, 1478-1487.
  • Şengül, M., Topdaş, E.F., Doğan, H., Serencam, H. 2018. Artvin İlinde Geleneksel Olarak Üretilen Bazı Marmelat Çeşitlerinin Çeşitli Fiziksel ve Kimyasal Özellikleri ile Antioksidan Aktiviteleri ve Fenolik Profillerinin Araştırılması. Akademik Gıda, 16, 1, 51-59.
  • Wan-Ibrahim, W.I., Sidik, K. and Kuppusamy, U.R. 2010. A high antioxidant level in edible plants is associated with genotoxic properties. Food Chemistry, 122, 4, 1139- 1144.
  • Yilmaz, K.U., Ercisli, S., Cam, M., Uzun, A., Yilmaztekin, M., Kafkas, E., Pinar, H. 2015. Fruit Weight, Total Phenolics, Acidity and Sugar Content of Edible Wild Pear (Pyrus elaeagnifolia Pall.) Fruits. Erwerbs-Obstbau, 57, 4, 179-184.
  • Young I.S., Woodside J.V. 2001. Antioxidants in Health and Disease. J Clin Pathol. 54, 3, 176-186.
Year 2019, , 447 - 456, 24.03.2019
https://doi.org/10.18185/erzifbed.489779

Abstract

References

  • Abacı, Z.T., Sevindik, E., Ayvaz, M. 2016. Comparative study of bioactive components in pear genotypes from Ardahan/Turkey. Biotechnology & Biotechnological Equipment, 30, 1, 36-43.
  • Anonim 2013. Ahlat bitkisi nedir, nasıl kullanılır? (http://www.hastalıktedavi.net), Son erişim tarihi: 19.11.2018
  • Anonim 2017. https://www.1organik.com/ahlat-agaci-ahlat-armudu.html, Son erişim tarihi: 19.11.2018
  • AOAC, 1990. Official methods of analysis of the Association of Official Analytical Chemists, 15th ed., Arlington VA, 1058-1059.
  • Aslan, G., Yazıcı, K. 2017. Doğanın Bize Sunduğu Görsel Şölen Yabani Meyveler I. Türktob, 21, 60-64.
  • Bakhshi, D., Arakawa, O. 2006. Effects of UV-b irradiation on phenolic compound accumulation and antioxidant activity in ‘Jonathan’ apple influenced by bagging, temperature and maturation. Journal of Food, Agriculture & Environment, 4, 1, 75-79.
  • Baltas, N. 2017. Investigation of a wild pear species (Pyrus elaeagnifolia subsp. Elaeagnifolia Pallas) from Antalya, Turkey: polyphenol oxidase properties and anti-xanthine oxidase, anti-urease, and antioxidant activity. International Journal of Food Properties, 20, 3, 585-595.
  • Benzie, I.F.F, Strain, J.J. 1996. The ferric reducing Ability of plasma (FRAB) as a measure of Antioxidant power: The FRAB assay. Analytical Biochemistry, 239, 70-76.
  • Collins, C.M., Lyne, P.M., Grange, J.M., 1989. Antimicrobial sensitivity and assay tests. Collins and Lyne’s Microbiological Methods. Butterworths, London Coşkun, T., 2005. Fonksiyonel besinlerin sağlığımız üzerine etkileri. Çocuk Sağlığı ve Hastalıkları Dergisi, 48, 69-84.
  • Çakılcıoğlu, U., Şengün, M.T., Türkoğlu, D. 2010. An ethnobotanical survey of medicinal plants of Yazıkonak and Yurtbaşı districts of Elazığ province, Turkey. Journal of Medicinal Plants Research, 4, 7, 567-572.
  • Heimler, D. Vignolini, P. Dini, M.G. Vincieri, F.F. Pomani, A. 2006. Antiradical activity and polyphenol composition of local Brassicaiceae adible varieties. Food Chemistry, 99, 464-469.
  • Erbil, N., Murathan, Z. T., Arslan, M., Ilcim, A., Sayin, B. 2018. Antimicrobial, Antioxidant, and Antimutagenic Activities of Five Turkish Pear Cultivars. Erwerbs-Obstbau, 60, 3, 203-209.
  • Güdücü, F. 2014. Pyrus elaeagrifolia bitkisi ekstrelerinin fenolik madde içerikleri, DPPH radikali giderme aktiviteleri ve in vitro antimikrobiyal etkilerinin belirlenmesi. Master's thesis, Trakya Üniversitesi.
  • Justesen, U., Knuthsen, P., Leth, T. 1998. Quantitative analysis of flavonols, flavones, and flavonones in fruits, vegetables and beverages by high-performance liquid chromatography with photodiode array and massspectrometric detection. Journal of Chromatography A, 799, 101-110.
  • Keçeci, L. D. 2017. Hakkari yöresi üstün nitelikli ahlat (Pyrus elaegrifolia L.) genotiplerinin bazı özelliklerinin belirlenmesi. Master's thesis, Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü.
  • Maron, D., Ames, B. 1983. Revised Methods for the Salmonella Mutagenicity Test. Mutation Research, 113, 173-215.
  • Mbaebie, B.O., Edeoga, H.O., Ajelayan, A.J. 2012. Phytochemical analysis and antioxidant activities of aqueous bark extract of Schoba lahtolic JACQ. Asian Pacific Journal of Tropical Biomedicine, 2, 118-124.
  • Naczk, M., Shahidi, F. 2006 Phenolics in cereals, fruits and vegetables: Occurence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis 41, 1523-1542.
  • Nizamoğlu, N.M., Nas, S. 2010. Meyve ve Sebzelerde Bulunan Fenolik Bileşikler; Yapıları ve Önemleri. Gıda Teknolojileri Elektronik Dergisi, 5, 1, 20-35. Özbek, S. 1985. Genel Meyvecilik. Çukurova Üniversitesi Ziraat Fak. Yayınları Ders Kitabı, 386.
  • Parejo, L., Codina, C., Petrakis, C., Kefalas, P. 2000. Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH center dot (2,2-diphenyl-1- picrylhydrazyl) free radical assay. Journal of Pharmacological and Toxicological Methods, 44, 507-512.
  • Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, J., Luyck, M., Cazin, M., Cazin, J.C., Bailleul, F., and Trotin, F. 2000. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72, 35-40.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26, 9/10, 1231-1237.
  • Sanchez-Moreno C, Plaza L, De Ancos B, et al. 2003. Quantitative bioactive compounds assessment and their relative contribution to the antioxidant capacity of commercial orange juices. J Sci Food Agr. 83, 430-439.
  • Sedlak, J., Lindsay, R.H. 1968. Estimation of Total Protein-Bound, and Nonprotein Sulfhydryl Groups in Tissue with Ellman's Reagent. Analytical Biochemistry, 25, 1, 192-205.
  • Smatana, L., Kytka, J., Kadarova, S. 1988. Results of Breeding and Groving Minor Fruit Species in Czechoslovakia. Acta Horticulture, 224, 83-87.
  • Smirnoff N. 1996 The function and metabolism of ascorbic acid in plants. Ann Bot. 78, 661-669.
  • Smirnova, G.V., Oktyabrsky, O.N. 2005. Glutathione in Bacteria. Biochemistry (Moscow), 70 11, 1199-1211.
  • Spanos, G.A., Wrolstad, R.E. 1992. Phenolic of apple, pear and white grape juices and their changes with processing and storage. Journal of Agriculture and Food Chemistry, 40, 9, 1478-1487.
  • Şengül, M., Topdaş, E.F., Doğan, H., Serencam, H. 2018. Artvin İlinde Geleneksel Olarak Üretilen Bazı Marmelat Çeşitlerinin Çeşitli Fiziksel ve Kimyasal Özellikleri ile Antioksidan Aktiviteleri ve Fenolik Profillerinin Araştırılması. Akademik Gıda, 16, 1, 51-59.
  • Wan-Ibrahim, W.I., Sidik, K. and Kuppusamy, U.R. 2010. A high antioxidant level in edible plants is associated with genotoxic properties. Food Chemistry, 122, 4, 1139- 1144.
  • Yilmaz, K.U., Ercisli, S., Cam, M., Uzun, A., Yilmaztekin, M., Kafkas, E., Pinar, H. 2015. Fruit Weight, Total Phenolics, Acidity and Sugar Content of Edible Wild Pear (Pyrus elaeagnifolia Pall.) Fruits. Erwerbs-Obstbau, 57, 4, 179-184.
  • Young I.S., Woodside J.V. 2001. Antioxidants in Health and Disease. J Clin Pathol. 54, 3, 176-186.
There are 32 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Makaleler
Authors

Zehra Tuğba Murathan 0000-0002-1468-7240

Nurcan Erbil

Vesile Düzgüner

Mehmet Arslan

Publication Date March 24, 2019
Published in Issue Year 2019

Cite

APA Murathan, Z. T., Erbil, N., Düzgüner, V., Arslan, M. (2019). Şakok Armudunun (Pyrus elaeagnifila Pallas) Antioksidan, Antimikrobiyal ve Mutajenik Özelliklerinin İncelenmesi. Erzincan University Journal of Science and Technology, 12(1), 447-456. https://doi.org/10.18185/erzifbed.489779

Cited By

Investigation of the local pear genotypes grown in Malatya
International Journal of Agriculture Environment and Food Sciences
https://doi.org/10.31015/jaefs.2024.1.1