Abstract
Polygalaceae kozmopolit bir yayılıma sahip, 27 cins içinde yaklaşık 1,200 türe sahip geniş bir familyadır. Ancak, birçok bitki grubu gibi, Polygalaceae türlerinin tanımlanması genellikle çiçek ve meyva karakterlerine bağlıdır, ki bu nedenle DNA barkodlama steril materyalin doğru tanımlanmasını sağlayabilir. Bu çalışmada altı tane yaygın olarak kullanılan DNA barkodu, rbçL, matK, trnL-F DNA bölgesi (trnL intron+trnL-F intergenic spacer dahil), tüm ITS (ITS1+5.8S+ITS2), subunits ITS1 and ITS2 DNA bölgelerinin, Maximum Likelihood (ML) ve TAXONDNA yöntemleri kullanılarak DNA barkodu olarak performansları değerlendirilmiştir. Sonuçlar göstermiştir ki, altı DNA bölgesinden hiçbiri ideal değildir, ancak Polygalaceae familyası için en uygun DNA barkodu matK gen bölgesidir.
Keywords
References
- Alvarez, I., and Wendel, J.F. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogenet. Evol. 29(3): 417–434. doi:10.1016/S1055-7903(03)00208-2. PMID:14615184.
- Baldwin, B.G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the Compositae. Mol. Phylogenet. Evol. 1(1): 3–16. doi:10.1016/1055-7903(92)90030-K. PMID:1342921.
Abstract
Polygalaceae is a large family with a cosmopolitan distribution, comprising ca. 1,200 species in 27 genera. However, similar to many plant groups, the identification of Polygalaceae species mostly depends on floral and fruit characteristics; therefore, DNA barcoding could easily warrant the corect identification of sterile material. In the current study, the utility of six widely employed plant DNA barcode loci, namely rbcL, matK, trnL-F region (including trnL intron+trnL-F intergenic spacer), the entire ITS (ITS1+5.8S+ITS2) as well as subunits ITS1 and ITS2 have been explored by performing Maximum Likelihood (ML) and TaxonDNA analyses. The results have shown that, while none of the six loci reviewed here completely fulfils the ideal DNA barcoding criteria; yet, matK region is the most useful DNA barcode for Polygalaceae.
Keywords
References
- Alvarez, I., and Wendel, J.F. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogenet. Evol. 29(3): 417–434. doi:10.1016/S1055-7903(03)00208-2. PMID:14615184.
- Baldwin, B.G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the Compositae. Mol. Phylogenet. Evol. 1(1): 3–16. doi:10.1016/1055-7903(92)90030-K. PMID:1342921.
Details
| Primary Language | English |
|---|---|
| Subjects | Engineering |
| Journal Section | Makaleler |
| Authors | |
| Publication Date | March 31, 2021 |
| Published in Issue | Year 2021 |