EFFECT OF SOME DRUGS ON SHEEP LIVER CARBONIC ANHYDRASE I AND II

Abstract

Effects of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) were examined on purified sheep carbonic anhydrase I and II (SCAI, SCA-II). In our study, we were chosen sheep liver tissue; because the liver is responsible for detoxification of xenobiotics. The CA isoenzyme have a very important duties on organisms, the effect of xenobiotics on the CA enzyme was reported. We exhibited the inhibition effects of these drugs against two sheep liver carbonic anhydrase isozymes (SCA I and SCA II). SCA I and SCA II isozymes were purified with about a specific activity  of 1108,51 EU/mg and 5706,67 EU/mg,  120,62 and  620,96 purification fold by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA I and SCA II isoenzyme was checked by SDS-PAGE technique and subunit molecular masses of the SCA I was found as 29 kDa and SCA II found as 31 kDa. The usage of clindamycin, dexketoprofen trometamol, Benzylpenicillin, (penicillin G), Phenoxymethylpenicillin (penicillin V) in sheep liver especially during necrotic hepatitis disease is important for scientific researches. As most of the antibiotic and antihistaminic can be dangerous for sheep liver. Also, it is important to study the inhibition effects of these drugs on SCA I and II izoenzymes.

Keywords

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BAZI İLAÇLARIN KOYUN KARACİĞERİ KARBONİK ANHİDRAZ I VE II ÜZERİNDEKİ ETKİSİ

Abstract

Koyun karaciğerinden saflaştırılmış karbonik anhidraz I ve II (SCAI, SCA-II) üzerine; klindamisin, deksketoprofen trometamol, benzilpenisilin (penisilin G) ve fenoksimetilpenisilin (penisilin V)’in etkileri incelenmiştir. Çalışmamızda koyun karaciğer dokusunu seçtik, çünkü karaciğer ksenobiyotiklerin detoksifikasyonundan sorumludur. Karbonik anhidraz izoenzimlerinin ksenobiyotiklerin karbonik anhidraz üzerindeki etkileri rapor edilmiştir. organizmalarda çok önemli görevleri vardır, Çalışmamızda bu ilaçların, koyun karaciğeri karbonik anhidraz I ve II izoenzimlerine karşı inhibisyon etkisi gösterdiği gözlenmiştir. SCA I ve SCA II kromatografisi kullanılarak, yaklaşık olarak 1108.51 EU/mg ve 5706.67 EU/mg spesifik aktivite ile 120.62 ve 620.96 kat saflaştırıldı. SCA I ve SCA II izoenzimlerinin saflığı SDS-PAGE tekniği ile tespit edildi ve alt birimlerinin ağırlığı SCA I’de 29 kDa ve SCA II’de 31 kDa olarak belirlendi. Koyun karaciğerinde, özellikle nekrotik hepatitis hastalığı sırasında kullanılan; klindamisin, deksketoprofen trometamol, benzilpenisilin (penisilin G) ve fenoksimetilpenisilin (penisilin V) hakkında önemli araştırmalar yapılmaktadır. Birçok antibiyotik ve antihistamin koyun karaciğeri için tehlikeli etkilere sahip olabilir. Ayrıca, bu ilaçların SCA I ve II izoenzimleri üzerinde inhibisyon etkilerini incelemek oldukça önemlidir

Keywords

• Karaciğer, Antibiyotik, İnhibisyon

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