The Effect of Different Doses of Fluopyram on the Kidney Tissues of Mice

Year 2021, , 970 - 978, 18.12.2021

Meltem Özgöçmen , Vehbi Atahan Toğay

https://doi.org/10.18185/erzifbed.969104

Abstract

Fluopyram is a widely used new generation broad spectrum fungicide and its toxic effects are mostly unknown. In this study, it was aimed to observe the effect of fluopyram administration at different doses on mouse kidney tissues. 0.5, 1 and 2 mg/kg Fluopyram were administered to mice, their kidneys were taken and evaluated histopathologically and immunohistochemically. Hematoxylin-eosin staining and also immunostaining with caspase-3, TNF-α, NF-Kb antibodies was performed. When the control group and fluopyram administered groups were compared, significant differences were found (p<0.05). While the kidney tissue of the control group was in normal histological structure, histopathological findings were observed in the experimental groups. When fluopyram administration groups were compared among themselves, it was observed that toxicity increased in a dose-dependent manner (p<0.05). No positive staining was observed with caspase-3, TNF-α, NF-kB in the tissues of the control group, however, a dose-dependent increase in positive staining was observed for all three stainings in the all fluopyram-treated groups (p<0.05). In conclusion, fluopyram appears to cause dose-dependent increased toxicity in mouse kidney tissues.

Keywords

Caspase-3, Fluopyram;, Histopathology, NF-kB, TNF-α

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