Determination of Asemetacin in Drugs by Flow-Injection Analysis Technique Using UV Detector

Year 2019, Volume: 12 Issue: 2, 1008 - 1017, 31.08.2019

Gülşah Saydan Kanberoğlu , Erol Şener , Muzaffer Tuncel Zühre Şentürk

https://doi.org/10.18185/erzifbed.536965

Abstract

In this study, a flow-injection analysis (FIA) system with UV-detection
is described for the direct determination of Acemetacin, which is
antiinflammatory-analgesic drug used for the treatment of rheumatic diseases.
The best suitable carrier solvent system was found to be 2 mM NaOH prepared in
ethanol :water (20:80, v/v). Using a flow-rate of 1,2 mL min^-1 and
wavelength of 280,8 nm, the linear response was obtained for Acemetacin in the
range of 1,2×10^-5‒5,8×10^-5 M (5,0‒24,1 μg mL^-1)with a detection limit of 1,6×10^-6 M
(0,7 μg mL^-1). The proposed method was successfully applied to the
determination of Acemetacin in commercial capsules and results were compared by
a simple UV-spectrophotometric method developed in this study.

Akış-Enjeksiyon Analiz Tekniği ile UV Dedektör Kullanılarak İlaçlarda Asemetasin Tayini

Abstract

Bu çalışmada romatizma
tedavisinde kullanılan antiinflamatuvar-analjezik etkili ilaçlardan Asemetasin
bileşiğinin doğrudan tayininde UV-dedektörlü akış-enjeksiyon analiz (FIA)
tekniği tanımlanmıştır. En uygun taşıyıcı çözücünün, etanol:su (20:80, h/h) içerisinde
hazırlanan 2 mM NaOH olduğu saptanmıştır. Akış hızı olarak 1,2 mL dak^-1
ve çalışma dalga boyu olarak 280,8 nm değerleri kullanıldığında Asemetasin için
1,2×10^-5‒5,8×10^-5 M (5,0‒24,1 μg mL^-1)derişim
aralığında, 1,6×10^-6 M (0,7 μg mL^-1) gözlenebilirlik sınırına
sahip doğrusal bir aralık elde edilmiştir. Önerilen yöntem, bu bileşiği içeren
ticari kapsül ilaç şekline başarıyla uygulanmış ve sonuçlar bu çalışmada
geliştirilen basit bir UV-spektrofotometrik yöntemle karşılaştırılmıştır.

Keywords

Asemetasin, akışa enjeksiyon analizi, UV dedektör, miktar tayini

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