Determination of Asemetacin in Drugs by Flow-Injection Analysis Technique Using UV Detector

Year 2019, Volume: 12 Issue: 2, 1008 - 1017, 31.08.2019

Gülşah Saydan Kanberoğlu Erol Şener Muzaffer Tuncel Zühre Şentürk

https://doi.org/10.18185/erzifbed.536965

Abstract

In this study, a flow-injection analysis (FIA) system with UV-detection
is described for the direct determination of Acemetacin, which is
antiinflammatory-analgesic drug used for the treatment of rheumatic diseases.
The best suitable carrier solvent system was found to be 2 mM NaOH prepared in
ethanol :water (20:80, v/v). Using a flow-rate of 1,2 mL min^-1 and
wavelength of 280,8 nm, the linear response was obtained for Acemetacin in the
range of 1,2×10^-5‒5,8×10^-5 M (5,0‒24,1 μg mL^-1)with a detection limit of 1,6×10^-6 M
(0,7 μg mL^-1). The proposed method was successfully applied to the
determination of Acemetacin in commercial capsules and results were compared by
a simple UV-spectrophotometric method developed in this study.

Akış-Enjeksiyon Analiz Tekniği ile UV Dedektör Kullanılarak İlaçlarda Asemetasin Tayini

Abstract

Bu çalışmada romatizma
tedavisinde kullanılan antiinflamatuvar-analjezik etkili ilaçlardan Asemetasin
bileşiğinin doğrudan tayininde UV-dedektörlü akış-enjeksiyon analiz (FIA)
tekniği tanımlanmıştır. En uygun taşıyıcı çözücünün, etanol:su (20:80, h/h) içerisinde
hazırlanan 2 mM NaOH olduğu saptanmıştır. Akış hızı olarak 1,2 mL dak^-1
ve çalışma dalga boyu olarak 280,8 nm değerleri kullanıldığında Asemetasin için
1,2×10^-5‒5,8×10^-5 M (5,0‒24,1 μg mL^-1)derişim
aralığında, 1,6×10^-6 M (0,7 μg mL^-1) gözlenebilirlik sınırına
sahip doğrusal bir aralık elde edilmiştir. Önerilen yöntem, bu bileşiği içeren
ticari kapsül ilaç şekline başarıyla uygulanmış ve sonuçlar bu çalışmada
geliştirilen basit bir UV-spektrofotometrik yöntemle karşılaştırılmıştır.

Keywords

Asemetasin, akışa enjeksiyon analizi, UV dedektör, miktar tayini

References

• Brooks, P.M., Day, R.O., 1991. ′′Nonsteroidal antiinflammatory drugs - differences and similarities′′, The New England Journal of Medicine, 324, 1716-1725.
• Castro, B, Gameiro, P., Lima, J.L.F.C., Matos, C., Reis, S., 2001.′′Interaction of drugs with hexadecylphosphocholine micelles. Derivative spectroscopy, acid-base and solubility studies′′, Materials Science and Engineering C, 18(1-2), 71-7.
• Duffy, C.P., Elliott, C.J., O′Connor, R.A., Heenan, M.M., Coyle, S., Cleary, I.M., Kavanagh, K., Verhaegen, S., O′Loughlin, C.M., Nicamhlaoibh, R., Clynes, .M. 1998. ′′Enhancement of chemotherapeutic drug toxicity to human tumour cells in vitro by a subset of non-steroidal anti-inflammatory drugs (NSAIDs)′′, European Journal of Cancer, 34(8), 1250-1259.
• Escuder-Gilabert, L., Martin-Biosca, Y., Sagrado, S., Villanueva-Camanas, R.M., Medina-Hermandez, M.J., 2002. ′′Quality control of pharmaceuticals containing non-steroidal anti-inflammatory drugs by micellar liquid chromatography′′, Chromatohraphia, 55, 283-288.
• Hoffmann, O., Klaushofer, K., Koller, K. 1985. ′′Prostaglandin –related bone resorption in cultured neonatal Mouse calvaria: Evaluation of biopotency of nonsteroidal anti-inflammatory drugs′′, Prostaglandins, 30 (5), 857-866.
• Ibrahim, H., Boyer, A., Bouajila, J., Couderc, F., Nepveu, F., 2007. ′′Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection′′, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 857(1), 59-66.
• Jones, R.W., Collins, A.J., Notarianni, L.J., Sedman, E., 1991. ′′The comparative pharmacokinetics of acemetacin in young subjects and elderly patients′′, British Journal of Clinical Pharmacology, 31, 543-545.
• Kayaalp, O. 2002 ′′Rasyonel tedavi yönünden tıbbi farmakoloji′′ 10. Baskı. Hacettepe-Taş Kitapçılık Ltd.Şti., Ankara, 960-994.
• Krull, I.S., Swartz, M., 1999. ′′Analytical method development and validation fort he academic researcher′′, Analytical Letters , 32 (6), 1067-1080.
• Martínez-Algaba, C., Escuder-Gilabert, L., Sagrado, S., Villanueva-Camañas, R.M., Medina-Hernández, M.J., 2004. ′′Comparison between sodium dodecylsulphate and cetyltrimethylammonium bromide as mobile phases in the micellar liquid chromatography determination of non-steroidal anti-inflammatory drugs in pharmaceuticals′′, Journal of Pharmaceutical and Biomedical Analysis, 36(2), 393-399.
• Notarianni, L.J., Collins, A. 1987. ′′Method forthe determination of acemetacin a non-steroidal anti-inflammatory drug in plasma by high –performans liquid chromatography′′, J of Chromatography. 413, 305-308.
• Özbakan, H., Murat Palabiyik, I., Gökhan Çaǧlayan, M., Onur, F., 2009. ′′Spectrophotometric and liquid chromatographic determination of acemetacin in pharmaceutical formulations′′, Turkish Journal of Pharmaceutical Sciences, 6(3), 163-176.
• Palacios, J.L., Arcos, J., Batanero, P.S. 1988. ′′Electrochemical behaviour of acemetacin′′, Journal of Pharmaceutical and Biomedical Analysis, 6, 963-968.
• Paraskevas D.T., Demetrius G.T., 1991.′′Review of recent applications of flow injection spectrophotometry to pharmaceutical analysis′′, Analytica Chimica Acta 588, 1-9.
• Reguera, C., Ortiz, M.C., Arcos, M. J. 2002. ′′Differantial pulse voltammetric simultaneous determination of four anti-inflammatory drugs by using soft modelling′′, Electroanalysis. 14(24), 699-1706.
• Saul, P.A., Korlipara, K. 1991. ′′Acemetacin and indometacin in the treatment of rheumayoid-arthritis a double-blind comparative study in general practice′′, Current Medical Research and Opinion, 12, 332-341.
• Schöllnhommer, G., Dell, H.D., Doersing, K., Kamp, R. 1985. ′′Quanitative determination of acemetacin and its metabolite indometacin in blood and plasma by column liquid chromatography′′, Journal of Chromatography B: Biomedical Sciences and Applications,375, 331-338.
• Yuan, Y., Sun, N., Yan, H., Han, D., Row, K.H., 2016. ′′Determination of indometacin and acemetacin in human urine via reduced graphene oxide - based pipette tip solid-phase extraction coupled to HPLC′′, Microchimica Acta, 183(2), 799-804.
• Zhang, Z.-J., Zhuang, Y.-F., Ju, H.-X., 2004.′′Flow injection chemiluminescent detection of acemetacin in KMnO4 - Na2SO3 system′′, Yaoxue Xuebao, 39(11), 925-928.