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The Effects of Combined İnsecticide and Salt Stresses on Some Physiological and Biochemical Parameters of Pepper Plant (Capsicum annuum L.)

Year 2019, Volume: 12 Issue: 3, 1601 - 1612, 31.12.2019
https://doi.org/10.18185/erzifbed.622294

Abstract

Insecticides are the pesticides that are commonly used to
kill insect in agriculture. Metaflumizone is a commonly used insecticide in
vegetable cultivation . The aim of this study is to determine the changes on
pepper plant due to metaflumizone application and to investigate the effect of
salt stress on these changes. For this purpose 50 mM NaCl and different
concentrations of metaflumizone (1.2, 2.4 ve 4.8 mM) was used as a stress
factor in the seperate and simultaneous applications at the pepper. 1.2 mM
metaflumizone application did not cause significant changes in plants.
Meanwhile, high concentrations of metaflumizone decreased % RWC, total
chlorophyll, and cartenoid while increasing APX, GST, GR activities as well as
GSH, MDA, and prolin contents. Application of NaCl to the plants simultaneously
with metaflumizone induced changes caused by insecticide. This study showed
that the insecticide and salt stresses show synergistic effect and therefore
the salt stress simultaneously with metaflumizone increases the insecticide
effect.

References

  • Akbulut, GB., Yigit, E., Kaya, A., Aktas, A. 2018. “Effects of salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl”, Ecotoxicology and Environmental Safety, 148, 901–909.
  • Akerboom, T.P.M., Sies, H. (1981). “Assay of glutathione, glutathione disulfide and glutathione mixed disulfide in biological samples”, In: Jakoby, WB (ed.), Methods Enzymol. Academic Press, New York, 77, 373–382.
  • Akgül, H. 2003. “Tuzluluk”. Ziraat Mühendisliği Dergisi, No: 340.
  • Altıkat, A., Turan, T., Torun, F.E. 2009. “Türkiye’de pestisit kullanımı ve çevreye olan etkileri”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 40 (2), 87-92.
  • Andrews, C.J. 2005. “Purifcation and characterisation of a family of glutathione transferases with roles in herbicide detoxifcation in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners”. Pesticide Biochemistry and Physiology, 82, 205–219.
  • Barr, H.D., Weatherley, P.E. 1962. “Are-examination of the relative turgidity technique for estimating water deficit inleaves”, Australian Journal of Biological Sciences Society, 15, 413–428.
  • Bates, L.S., Waldren, R.P., Teare, I.D. 1973.” Rapid determination of proline for water-studies”, Plant and Soil, 39, 205-207.
  • Bertrand, L., Marino, D.J., Monferrán, M.V., Amé, M.V. 2017. “Can a low concentration of an organophosphate insecticide cause negative effects on an aquatic macrophyte? Exposure of Potamogeton pusillus at environmentally relevant chlorpyrifos concentrations”, Environmental and Experimental Botany, 138, 139–147.
  • Bradford, M.M. 1976. “A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding”, Analytical Biochemistry, 72, 248–254.
  • Cakmak, I. 1994. “Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium-deficient and potassium deficient leaves, but not in phosphorus-deficient leaves”, Journal of Experimental Botany, 45, 1259–1266.
  • Carlberg, I., Mannervik, B. (1985). “Glutathione reductase”, Methods in Enzymology, 113, 484– 490.
  • Çanakçı, S., Munzuroğlu, M. 2004. “Fasulye (Phaseolus vulgaris L.) çeliklerinde ağırlık değişimleri, pigment ve protein miktarları üzerine asetilsalisilik asit ve tuz (NaCl) uygulamasının karşılıklı etkileri”, Gazi Eğitim Fakültesi Dergisi, 24(1), 23-40.
  • Çulha, Ş., Çakırlar, H. 2011. “Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları”, AKU Fen Bilimleri Dergisi, 11, 11-34.
  • De-Kok, L., Graham, M. 1980. “Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark induced and natural senescence”, Plant Physiology and Biochemistry, 27, 133-142.
  • Delauney, A.J., Verma, D.P.S., 1993. “Proline biosynthesis and osmoregulation in plants”, The Plant Journal, 4(2), 215-223.
  • Dong, F., Liu, X., Cheng, L., Chen, W., Li, J., Qin, D., Zheng, Y. 2009. “Determination of metaflumizone residues in cabbage and soil using ultra-performance liquid chromatography/ESI-MS/MS”, Journal of Separation Science, 32, 3692–3697.
  • Duncan, D.B. 1955. “Multiple range and multiple F tests biometrics. International Biometric Society”, 11(1), 1–42.
  • Habig, W.H., Pabst, M.J., Jakoby, W.B. 1974. “The first enzymatic step in mercapturic acid formation Glutathion S-Transferases”, Journal of Biological Chemistry, 249, 7130–7139.
  • Hancı, F., Cebeci, E. 2014. “Investigation of proline, chlorophyll and carotenoids changes under drought stress in some onion (Allium cepa L.) cultivars”, Turkish Journal of Agricultural and Natural Sciences Special Issue: 2, 1499-1504.
  • Heath, R.L., Packer, L. 1968. “Photoperoxidation in isolated chloroplast, I. kinetics stoichiometry of fatty acid peroxidation”, Archives of Biochemistry and Biophysics, 125, 180–198.
  • Jiang, L., Yang, H. 2009. “Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat”, Ecotoxicology and Environmental Safety, 72, 1687–1693.
  • Kaya, A., Inan. M. 2018. “Kuraklık ve Tuz Streslerine Maruz Kalan Tütün (Nicotiana tabacum L.) Bitkisinde Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Melatoninin Etkileri”. KSÜ Tarım ve Doğa Dergisi, 21(4), 559-564.
  • Kaya, C., Higgs, D. 2006. “Supplementary potassium nitrate ımproves salt tolerance in bell Pepper Plants”, Journal of Plant Nutrition, 26(7), 1367–1382.
  • Kaya, C., Higgs, D., Kirnak, H. 2001. “The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach”, Bulgarian Journal of Plant Physiology, 27(3-4), 47–59.
  • Kaymak, S., Özdem, A., Karahan, A., Özercan, B., Aksu, P., Aydar, A., Kodan, M., Yılmaz, A., Başaran, S., Asav, Ü., Erdoğan, P., Güler, Y. 2015. “Ülkemizde zirai mücadele girdilerinin değerlendirilmesi”, TC Gıda, Tarım ve Hayvancılık Bakanlığı.
  • Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., Jia, D., Fu, M., Ma, F. 2012. “The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis”, Journal of Pineal Research, 53, 298–306.
  • Lichtenthaler, K., Welburn, A.R. 1983. “Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents”. Botanisches Institutder Univeristat, Kaiserstran βe 12, Postfach pp. 591-592.
  • Mittler, R. 2002. “Oxidative stress, antioxidants and stress tolerance”, Trends Plant Science, 7(9), 405–410.
  • Nakano, Y., Asada, K. 1981. “Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts”, Plant and Cell Physiology, 22, 867–880.
  • Oncel, I., Keleş, Y. 2002. “Tuz stresi altındaki buğday genotiplerinde büyüme, pigment içeriği ve çözünür madde kompozisyonunda değişmeler”, CÜ. Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 23, 8-16.
  • Salgado, V.L., Hayashi, J.H. 2007. “Metaflumizone is a novel sodium channel blocker insecticide”, Veterinary Parasitology, 150, 182–189.
  • Santos, C.V. 2004. “Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves”, Scientia Horticulturae, 103, 93–99.
  • Sivaci, A., Kaya, A., Duman, S., 2014. “Effects of ascorbic acid on some physiological changes of pepino (Solanum muricatum Ait.) under chilling stress”, Acta Biologica Hungarica, 65(3), 305–318.
  • Tiryaki, O., Canhilal, R., Horuz, S. 2010. “Tarım ilaçları kullanımı ve riskleri”, ERÜ Fen Bilimleri Enstitüsü Dergisi, 26(2), 154-169.
  • Wang, C., Zhang, Q. 2017. “Exogenous salicylic acid alleviates the toxicity of chlorpyrifos in wheat plants (Triticum aestivum)”, Ecotoxicology and Environmental Safety, 137, 218–224.
  • Yakıt, S., Tuna, A.L. 2006. “Tuz stresi altındaki mısır bitkisinde (Zea mays L.) stres parametreleri üzerine Ca, Mg ve K’nın etkileri”, Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 19(1), 59-67.
  • Zhang, J.J., Lu, Y.C., Zhang, J.J., Tan, L.R., Yang, H. 2014. “Accumulation and toxicological response of atrazine in rice crops”, Ecotoxicology and Environmental Safety, 102, 105–112.

Eş Zamanlı Uygulanan İnsektisit ve Tuz Streslerinin Biber Bitkisinde (Capsicum annuum L.) Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Etkileri

Year 2019, Volume: 12 Issue: 3, 1601 - 1612, 31.12.2019
https://doi.org/10.18185/erzifbed.622294

Abstract

İnsektisiler
tarımsal üretimde böceklerle mücadelede kullanılan pestisitlerdir. Metaflumizone
özellikle sebze yetiştiriciliğinde yaygın olarak kullanılan bir insektisittir.
Bu çalışma metaflumizone uygulamasının biber bitkisinde meydana getirdiği
değişimleri belirlemeyi ve bu değişimler üzerine tuz stresinin etkilerini incelemeyi
amaçlamaktadır. Bu amaçla biberde 50 mM NaCl ve farklı dozlarda metaflumizone
(1.2, 2.4 ve 4.8 mM) streslerinin ayrı ayrı ve eş zamanlı uygulamalarının
etkileri incelenmiştir. 1.2 mM metaflumizone uygulaması bitkilerde önemli bir
değişime sebep olmamıştır. Bununla birlikte yüksek dozda metaflumizone
uygulaması % RWC, toplam klorofil ve karotenoid içeriklerinde azalmaya, APX,
GST, GR aktiviteleri ile GSH, MDA ve prolin içeriklerinde bir artışa sebep
olmuştur. Bitkilere metaflumizone ile eş zamanlı olarak NaCl uygulanması
insektisitin meydana getirdiği değişimleri indüklemiştir. Bu çalışma insektisit
ve tuz streslerinin sinerjistik etki gösterdiğini ve bu nedenle insektisit ile
eş zamanlı uygulanan tuz stresinin insektisit etkisi ile oluşan hasarı arttırdığını
ortaya çıkarmıştır.

References

  • Akbulut, GB., Yigit, E., Kaya, A., Aktas, A. 2018. “Effects of salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl”, Ecotoxicology and Environmental Safety, 148, 901–909.
  • Akerboom, T.P.M., Sies, H. (1981). “Assay of glutathione, glutathione disulfide and glutathione mixed disulfide in biological samples”, In: Jakoby, WB (ed.), Methods Enzymol. Academic Press, New York, 77, 373–382.
  • Akgül, H. 2003. “Tuzluluk”. Ziraat Mühendisliği Dergisi, No: 340.
  • Altıkat, A., Turan, T., Torun, F.E. 2009. “Türkiye’de pestisit kullanımı ve çevreye olan etkileri”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 40 (2), 87-92.
  • Andrews, C.J. 2005. “Purifcation and characterisation of a family of glutathione transferases with roles in herbicide detoxifcation in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners”. Pesticide Biochemistry and Physiology, 82, 205–219.
  • Barr, H.D., Weatherley, P.E. 1962. “Are-examination of the relative turgidity technique for estimating water deficit inleaves”, Australian Journal of Biological Sciences Society, 15, 413–428.
  • Bates, L.S., Waldren, R.P., Teare, I.D. 1973.” Rapid determination of proline for water-studies”, Plant and Soil, 39, 205-207.
  • Bertrand, L., Marino, D.J., Monferrán, M.V., Amé, M.V. 2017. “Can a low concentration of an organophosphate insecticide cause negative effects on an aquatic macrophyte? Exposure of Potamogeton pusillus at environmentally relevant chlorpyrifos concentrations”, Environmental and Experimental Botany, 138, 139–147.
  • Bradford, M.M. 1976. “A rapid and sensitive method for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding”, Analytical Biochemistry, 72, 248–254.
  • Cakmak, I. 1994. “Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium-deficient and potassium deficient leaves, but not in phosphorus-deficient leaves”, Journal of Experimental Botany, 45, 1259–1266.
  • Carlberg, I., Mannervik, B. (1985). “Glutathione reductase”, Methods in Enzymology, 113, 484– 490.
  • Çanakçı, S., Munzuroğlu, M. 2004. “Fasulye (Phaseolus vulgaris L.) çeliklerinde ağırlık değişimleri, pigment ve protein miktarları üzerine asetilsalisilik asit ve tuz (NaCl) uygulamasının karşılıklı etkileri”, Gazi Eğitim Fakültesi Dergisi, 24(1), 23-40.
  • Çulha, Ş., Çakırlar, H. 2011. “Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları”, AKU Fen Bilimleri Dergisi, 11, 11-34.
  • De-Kok, L., Graham, M. 1980. “Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark induced and natural senescence”, Plant Physiology and Biochemistry, 27, 133-142.
  • Delauney, A.J., Verma, D.P.S., 1993. “Proline biosynthesis and osmoregulation in plants”, The Plant Journal, 4(2), 215-223.
  • Dong, F., Liu, X., Cheng, L., Chen, W., Li, J., Qin, D., Zheng, Y. 2009. “Determination of metaflumizone residues in cabbage and soil using ultra-performance liquid chromatography/ESI-MS/MS”, Journal of Separation Science, 32, 3692–3697.
  • Duncan, D.B. 1955. “Multiple range and multiple F tests biometrics. International Biometric Society”, 11(1), 1–42.
  • Habig, W.H., Pabst, M.J., Jakoby, W.B. 1974. “The first enzymatic step in mercapturic acid formation Glutathion S-Transferases”, Journal of Biological Chemistry, 249, 7130–7139.
  • Hancı, F., Cebeci, E. 2014. “Investigation of proline, chlorophyll and carotenoids changes under drought stress in some onion (Allium cepa L.) cultivars”, Turkish Journal of Agricultural and Natural Sciences Special Issue: 2, 1499-1504.
  • Heath, R.L., Packer, L. 1968. “Photoperoxidation in isolated chloroplast, I. kinetics stoichiometry of fatty acid peroxidation”, Archives of Biochemistry and Biophysics, 125, 180–198.
  • Jiang, L., Yang, H. 2009. “Prometryne-induced oxidative stress and impact on antioxidant enzymes in wheat”, Ecotoxicology and Environmental Safety, 72, 1687–1693.
  • Kaya, A., Inan. M. 2018. “Kuraklık ve Tuz Streslerine Maruz Kalan Tütün (Nicotiana tabacum L.) Bitkisinde Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Melatoninin Etkileri”. KSÜ Tarım ve Doğa Dergisi, 21(4), 559-564.
  • Kaya, C., Higgs, D. 2006. “Supplementary potassium nitrate ımproves salt tolerance in bell Pepper Plants”, Journal of Plant Nutrition, 26(7), 1367–1382.
  • Kaya, C., Higgs, D., Kirnak, H. 2001. “The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach”, Bulgarian Journal of Plant Physiology, 27(3-4), 47–59.
  • Kaymak, S., Özdem, A., Karahan, A., Özercan, B., Aksu, P., Aydar, A., Kodan, M., Yılmaz, A., Başaran, S., Asav, Ü., Erdoğan, P., Güler, Y. 2015. “Ülkemizde zirai mücadele girdilerinin değerlendirilmesi”, TC Gıda, Tarım ve Hayvancılık Bakanlığı.
  • Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., Jia, D., Fu, M., Ma, F. 2012. “The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis”, Journal of Pineal Research, 53, 298–306.
  • Lichtenthaler, K., Welburn, A.R. 1983. “Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents”. Botanisches Institutder Univeristat, Kaiserstran βe 12, Postfach pp. 591-592.
  • Mittler, R. 2002. “Oxidative stress, antioxidants and stress tolerance”, Trends Plant Science, 7(9), 405–410.
  • Nakano, Y., Asada, K. 1981. “Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts”, Plant and Cell Physiology, 22, 867–880.
  • Oncel, I., Keleş, Y. 2002. “Tuz stresi altındaki buğday genotiplerinde büyüme, pigment içeriği ve çözünür madde kompozisyonunda değişmeler”, CÜ. Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 23, 8-16.
  • Salgado, V.L., Hayashi, J.H. 2007. “Metaflumizone is a novel sodium channel blocker insecticide”, Veterinary Parasitology, 150, 182–189.
  • Santos, C.V. 2004. “Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves”, Scientia Horticulturae, 103, 93–99.
  • Sivaci, A., Kaya, A., Duman, S., 2014. “Effects of ascorbic acid on some physiological changes of pepino (Solanum muricatum Ait.) under chilling stress”, Acta Biologica Hungarica, 65(3), 305–318.
  • Tiryaki, O., Canhilal, R., Horuz, S. 2010. “Tarım ilaçları kullanımı ve riskleri”, ERÜ Fen Bilimleri Enstitüsü Dergisi, 26(2), 154-169.
  • Wang, C., Zhang, Q. 2017. “Exogenous salicylic acid alleviates the toxicity of chlorpyrifos in wheat plants (Triticum aestivum)”, Ecotoxicology and Environmental Safety, 137, 218–224.
  • Yakıt, S., Tuna, A.L. 2006. “Tuz stresi altındaki mısır bitkisinde (Zea mays L.) stres parametreleri üzerine Ca, Mg ve K’nın etkileri”, Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 19(1), 59-67.
  • Zhang, J.J., Lu, Y.C., Zhang, J.J., Tan, L.R., Yang, H. 2014. “Accumulation and toxicological response of atrazine in rice crops”, Ecotoxicology and Environmental Safety, 102, 105–112.
There are 37 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Makaleler
Authors

Armağan Kaya 0000-0002-6776-3497

Publication Date December 31, 2019
Published in Issue Year 2019 Volume: 12 Issue: 3

Cite

APA Kaya, A. (2019). Eş Zamanlı Uygulanan İnsektisit ve Tuz Streslerinin Biber Bitkisinde (Capsicum annuum L.) Bazı Fizyolojik ve Biyokimyasal Parametreler Üzerine Etkileri. Erzincan University Journal of Science and Technology, 12(3), 1601-1612. https://doi.org/10.18185/erzifbed.622294