Arbutin’in yeni kaynakları: Onobrychis nitida and Onobrychis galegifolia

Year 2019, Volume: 12 Issue: 3, 1733 - 1737, 31.12.2019

Hüseyin Akşit , Samed Şimşek , Volkan Özdokur , Ekrem Köksal

https://doi.org/10.18185/erzifbed.658444

Cited By: 1

Abstract

Arbutin (4-Hidroksifenil-β-D-glukopiranozit),
hidrokinonun mono glikozidik formudur ve cilt beyazlatma ve çil giderme
özellikleri nedeniyle kozmetiklerde uzun süredir yaygın olarak
kullanılmaktadır. Bu çalışmada; arbutin flash kromatografi kullanılarak Onobrychis
galegifolia'nın metanol ekstraktından izole edilmiştir. Arbutin yapısı, ¹H
ve ¹³C -NMR spektroskopisi ve ayrıca 2D NMR teknikleri ile
aydınlatıldı. Ek olarak, O. galegifolia ve O. nitida türlerinde
bulunan arbutinin kantitatif analizi HPLC-DAD kullanılarak yapıldı. Sonuçlara
göre, O. galegifolia ve O. nitida'nın metanol ekstraktlarının
arbutin içeriği sırasıyla 212.02 ve 196.46 mg/g ekstrakt olarak bulundu.
Ayrıca, O. galegifolia ve O. nitida'nın soğuk su ekstraktlarının
arbutin içeriği 128.2 ve 99.40 mg/g ekstrakt olarak bulundu. Kuru
ağırlıklardaki arbutin miktarı, yaklaşık% 5.66 ila% 6.86 arasında hesaplandı.

Keywords

Onobrychis nitida, Arbutin

New sources of arbutin: Onobrychis nitida and Onobrychis galegifolia

Abstract

Arbutin
(4-Hydroxyphenyl-β-D-glucopyranoside) is a mono glycosidic form of
hydroquinone and has been widely used in cosmetics for a long time due to skin
whitening and freckle removing properties. In this study, arbutin was isolated
from the methanol extract of Onobrychis galegifolia using flash
chromatography. The structure of arbutin was elucidated by ¹H and ¹³C
-NMR spectroscopy and as well as 2D NMR techniques. In addition, quantitative
analysis of arbutin found in O. galegifolia and O. nitida species
was done using HPLC-DAD. According to the results, the arbutin content of
methanol extracts of O. galegifolia and O. nitida was found
212.02 and 196.46 mg/g extract respectively. In addition, the arbutin content
of cold-water extracts of O. galegifolia and O. nitida was found
128.2 and 99.40 mg/g extract. The amount of arbutin in dry weights was
calculated for about 5.66% to 6.86%.

Keywords

Arbutin, Onobrychis nitida

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