KANGAL BALIKLARININ (GARRA RUFA) SOLUNGAÇLARINDAKİ MUKUS HÜCRELERİNİN HİSTOKİMYASI ÜZERİNE ÇALIŞMA

Year 2009, Volume: 2 Issue: 1, 51 - 60, 04.03.2014

Dilek Diler , Kenan Çınar

Abstract

Bu çalışma, Kangal balıklarının (Garra rufa) solungaçlarındaki mukus hücrelerinin histokimyasal özelliklerini belirlemek amacıyla yapıldı. Primer lamel epitelinde bulunan mukus hücrelerinin asidik ve nötral glikokonjugatları, siyalik asit rezidüleri ve sülfatlı, çok sülfatlı ve O- sülfat esterli glikokonjugatları, sekonder lamele ait mukus hücrelerinin ise nötral glikokonjugat ile siyalik asit rezidüleri içerdiği belirlendi. Aynı zamanda PAS/AB pH 2.5 ve AF/AB pH 2.5 boyama yöntemlerinde primer lamel mukus hücrelerinde asidik glikokonjugatların baskın olduğu belirlendi.

Keywords

Garra rufa, glikokonjugat, mukus hücresi, solungaç

References

• Arellano, J.M., Storch, V., Sarasquete, C. (2004). Ultrastructure and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. J Appl Ichthyol. 20, 452–460.
• Calabró, C., Albanese, M.P., Lauriano, E.R., Licata, A., Martella, S., Concetta, C. (2005). Morphological histochemical and immunohistochemical study of the gill epithelium in the abyssal teleost fish Coelorhynchus coelorhynchus. Folia Histochem Cytobiol. 43, 51-56.
• Culling, C.F.A., Reid, P.E., Dunn, W.L. (1976). A new histochemical. method for the identification and visualisation of both side chain acylated and non-acylated sialic acids. J Histochem Cytochem. 24, 1225-1230.
• Çınar, K., Şenol N., Özen, M.R. (2008). Histochemical characterization of glycoproteins in the gills of the carp (Cyprinus carpio). Ankara Univ Vet Fak Derg. 55, 61-64.
• Díaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L. (2001). Mucous cells in Micropogonias furnieri gills: Histochemistry and ultrastructure. Anat Histol Embryol. 30, 135-139.
• Díaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L. (2005 a). Glycoconjugates in the branchial mucous cells of Cynoscion guatucupa (Cuvier, 1830) (Pisces: Sciaenidae). Sci Mar. 69, 545-553.
• Díaz, A.O., Garcia, A.M., Devincenti, C.V., Goldemberg, A.L. (2005 b). Ultrastructure and histochemical study of glycoconjugates in the gills of the white croaker (Micropogonias furnieri). Anat Histol Embryol. 34, 117-122.
• Domeneghini, C., Pannelli Straini, R., Veggetti, A. (1998). Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histol Histopathol. 13, 359-372.
• Fletcher, T.C. (1978). Defence mechanism in fish. J Histochem Cytochem. 32, 681-689.
• Gomori, G. (1952). Gomori’ s aldehyde fuchsin stain. (S. 238). (Editörler: C.F.A. Culling, R.T. Allison, W.T. Barr.) Cellular Pathology Technique. London: Butterworths.
• Lev, R., Spicer, S.S. (1964). Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem. 12, 309-309.
• McManus, J.F.A. (1948). Histological and histochemical uses of periodic acid. Stain Technol. 23, 99-108.
• Mowry, R.W. (1956). Alcian blue techniques for the histochemical study of acidic carbohydrates. J Histochem Cytochem. 4, 407-408.
• Shephard, K.L. (1994). Functions for fish mucus. Rev Fish Biol. 4, 401-429.
• Spicer, S.S., Mayer, D.R. (1960). Aldehyde fuchsin/ alcian blue. (S. 233). (Editörler: C.F.A. Culling, R.T. Allison, W.T. Barr.) Cellular Pathology Technique. London: Butterworths.
• Zayed, A.E., Mohamed, S.A. (2004). Morphological study on the gills of two species of fresh water fishes: Oreochromis niloticus and Clarias gariepinus. Ann Anat. 186, 295-304.
• http:www.tr.wikipedia.org/wiki/Kangal_bal%C4%B1%C4%9F%C4%B1 ****