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KAYNAK ALABALIĞI (SALVELINUS FONTINALIS)’NIN SPERMATOLOJİK ÖZELLİKLERİ VE SPERMANIN KISA SÜRELİ MUHAFAZASI

Year 2015, Volume: 8 Issue: 1, 88 - 99, 25.06.2015

Temel Şahin Özay Köse

https://doi.org/10.18185/eufbed.90287
Cited By: 1

Abstract

Bu çalışma kaynak alabalığının spermatolojik özelliklerini belirlemek ve spermanın kısa süreli muhafazası için sulandırıcı geliştirmek amacıyla yapılmıştır. Üreme sezonunda 6 erkek bireyden sperma abdominal masaj yöntemi ile alınmış ve spermalarda, miktar, motilite, motilite süresi, spermatokrit oranı, yoğunluk ve pH belirlenmiştir. Kaynak alabalığı spermalarında; miktar (ml), motilite (%), motilite süresi (s), spermatokrit oranı (%), yoğunluk (x 109 spermatozoon/ml) ve pH değerleri sırasıyla ortalama 4.3 ± 1.54, 98.3± 4.08, 59.2 ± 17.27, 27.1 ± 4.47, 12.9 ± 1.29 ve 6.9 ± 0.15 bulunmuştur. Sulandırıcı S1’de muhafaza edilen spermanın daha yüksek bir motiliteye sahip olduğu ve sulandırıcı S1’in diğerinden daha etkin olduğu görülmüştür.

Keywords

Kaynak Alabalığı Salvelinus fontinalis Sperma Motilite Sperma özellikleri

References

  • Alavi, S.M. and Cosson, J. 2005. Sperm motility in fishes. I.Effects of temperature and pH: a review. Cell. Biol. İnt, 29(2), 101-10.
  • Alavi, S.M.H., Pšenička, M., Policar, T., Rodina, M., Hamáčková, J., Pavel Kozák, P., Linhart, O. 2009. Sperm quality in male Barbus barbus L. fed different diets during the spawning season. Fish Physiol Biochem, 35, 683-693.
  • Aral, F., Şahinöz, E., Dogu, Z. 2007. A Study on the Milt Quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7, 41-44.
  • Babiak, I., Fraser, L., Dobosz, S., Goryczko, K., Kuzminski, H., Strzezek, J. 1999. Computercontrolled freezing of rainbow trout Onchorhynchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult. Res, 30, 707-710.
  • Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3, 247- 257.
  • Billard, R., Cosson, J., Perches, G., Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112.
  • Bozkurt, Y. 2004. Aynalı sazan (Cyprinus carpio L.1758) spermasının bazı spermatolojik özelliklerinin belirlenmesi ve farklı sulandırıcılar ile kısa süreli saklanmasının değerlendirilmesi. Doktora Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara, Türkiye, 168 sayfa.
  • Bozkurt, Y., Seçer, S., Tekin, N., Akçay, E. 2005. Cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) sperm with glucose based extender. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1), 21-25.
  • Bozkurt, Y., Seçer, S., Bekcan, S. 2006a. Relationship between spermatozoa motility, egg size, fecundity and fertilization success in Salmo trutta abanticus. Tarım Bilimleri Dergisi, 12 (4), 345-348.
  • Bozkurt, Y., Seçer, S., Bukan, N., Akçay, E., Tekin, N. 2006b. Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Sciences, 9(5), 940-944.
  • Bozkurt, Y., Öğretmen, F., Kökçü, Ö., Ercin, U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech J. Anim. Sci., 56(8), 355–364.
  • Büyükhatipoğlu, S. ve Holtz, W. 1977. Preservation of trout sperm in liqiud of frozen state. Aquaculture, 14, 96-56.
  • Canyurt, M.A., Akhan, S. 2008. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences, 8, 171-175.
  • Ciereszko, A., Dabrowski, K. 1993. Estimation of spermdensity of rainbow sperctrophotometric technique. Aquaculture, 109, 367-373. whitefish and yellow perch using a
  • Cloud, J.G., Miller, W.H., Levanduski, M.J. 1990. Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Prog. Fish Cult. 52, 51–53.
  • DeGraaf, J.D., Berlinsky, D.L. 2004. Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234(1–4), 527–540.
  • Geffen, A.J., Evans, J.P. 2000. Sperm traits and fertilization success of male and sex reversed female rain bow trout (Oncorhynchus mykiss). Aquaculture, 182, 61-72.
  • Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski, H., Ciereszko, A. 2000. Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Research, 31, 289-296.
  • Hatipoğlu, T., Akçay, E. 2010. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniv Vet Fak Derg, 57, 33-38.
  • Linhart, O., Rodina, M., Gela, D., Kocour, M. 2004. Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture, 235, 619–632.
  • Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T. 2006. Cryopreservation of chinese sturgeon (Acipenser sinensis) sperm. J. Appl. Ichthyol. 22(supp.1), 384-388.
  • Munkıttrıck, K.R., Moccıa, R.D. 1987. Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen, J. and Hyvarinen, H. 1983. Composition of milt of some teleost fish. Journal of Fish Biology, 22, 351-361.
  • Rurangwa, E., Kime, D.E., Ollevier, F., Nash, J.P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
  • Saad, A., Billard, R., Theron, M.C. and Hollebecq, M.G. 1988. Short- therm presevation of carp (Cyprinus carpio) semen. Aquaculture, 71, 133-150.
  • Suquet, M., Billard, R., Cosson, J., Dorange, G., Chauvaud, L., Mugnier, C., Fauvel, C. 1994. Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species. Aquatic Living Resources, 7, 283-294.
  • Suquet, M., Dreanno, C., Dorange, G., Normant, Y., Quemener, L., Gaignon, J.L., Billard, R. 1998. The aging phenomenon of turbot (Scophthalmus maximus) spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization and storage capacities. Journal of Fish Biology, 32, 31-41.
  • Şahin, T., Kurtoğlu, İ.Z. ve Köse, Ö. 2013. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın spermatolojik özellikleri ve spermanın kısa süreli muhafazası. Fırat Unv. Journal of Science, 25(1), 77-92.
  • Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y., Kayam, S. 2003. Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Türk J Vet Anim Sci, 27, 37–44.
  • Tuset, V.M., Dietrich, G.J., Wojtczak, M., Słowińska, M., de Monserrat, J., Ciereszko, A. 2008. Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa. J. Appl. Ichthyol, 24, 393–397.
  • DeGraaf, J.D., King, V.W., Benton, C., Berlinsky, D.L. 2004. Production and storage of sperm from the black sea bass Centropristis striata. Aquacult. Res., 35, 1457-1465.

-

Year 2015, Volume: 8 Issue: 1, 88 - 99, 25.06.2015

Temel Şahin Özay Köse

https://doi.org/10.18185/eufbed.90287
Cited By: 1

Abstract

This study was carried out to determine the spermatologic characteristics and to develop an extender for the short-term storage of semen from brook trout. Four different extenders were tested in the study: a) 8.75 g/l NaCl, 0.20 g/l KCl, 0.20 CaCl2, 0.30 g/l NaHCO3 (S1), b) 8.75 g/l NaCl, 0.20 g/l KCl, 0.20 MgCl2, 0.40 g/l NaHCO3 (S2), c) 7.25 g/l NaCl, 0.40 g/l KCl, 0.80 g/l NaHCO3, 2.0 g/l glucose (S3), d) 8.75 g/l NaCl, 0.20 g/l KCl, 0.10 CaCl2, 0.10 g/l MgCl2, 0.40 g/l NaHCO3 (S4). In the spawning season, sperm was collected by abdominal massage from 6 males. In collected sperm; volume, motility, duration of motility, spermatocrit, density and pH were determined. In the sperm of brook trout, volume (ml), motility (%), duration of motility (sec), spermatocrit, density (x 109spermatozoon/ml), and pH values were found as mean 4.3 ± 1.54, 98.3± 4.08, 59.2 ± 17.27, 27.1 ± 4.47, 12.9 ± 1.29 ve 6.9 ± 0.15, respectively. Sperm stored in extender S1 had a higher rate of motility and the extender S1 seemed to be more effective than the others

Keywords

Brook trout Salvelinus fontinalis Sperm Motility Spermatologic characteristics

References

  • Alavi, S.M. and Cosson, J. 2005. Sperm motility in fishes. I.Effects of temperature and pH: a review. Cell. Biol. İnt, 29(2), 101-10.
  • Alavi, S.M.H., Pšenička, M., Policar, T., Rodina, M., Hamáčková, J., Pavel Kozák, P., Linhart, O. 2009. Sperm quality in male Barbus barbus L. fed different diets during the spawning season. Fish Physiol Biochem, 35, 683-693.
  • Aral, F., Şahinöz, E., Dogu, Z. 2007. A Study on the Milt Quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7, 41-44.
  • Babiak, I., Fraser, L., Dobosz, S., Goryczko, K., Kuzminski, H., Strzezek, J. 1999. Computercontrolled freezing of rainbow trout Onchorhynchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult. Res, 30, 707-710.
  • Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3, 247- 257.
  • Billard, R., Cosson, J., Perches, G., Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112.
  • Bozkurt, Y. 2004. Aynalı sazan (Cyprinus carpio L.1758) spermasının bazı spermatolojik özelliklerinin belirlenmesi ve farklı sulandırıcılar ile kısa süreli saklanmasının değerlendirilmesi. Doktora Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara, Türkiye, 168 sayfa.
  • Bozkurt, Y., Seçer, S., Tekin, N., Akçay, E. 2005. Cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) sperm with glucose based extender. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1), 21-25.
  • Bozkurt, Y., Seçer, S., Bekcan, S. 2006a. Relationship between spermatozoa motility, egg size, fecundity and fertilization success in Salmo trutta abanticus. Tarım Bilimleri Dergisi, 12 (4), 345-348.
  • Bozkurt, Y., Seçer, S., Bukan, N., Akçay, E., Tekin, N. 2006b. Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Sciences, 9(5), 940-944.
  • Bozkurt, Y., Öğretmen, F., Kökçü, Ö., Ercin, U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech J. Anim. Sci., 56(8), 355–364.
  • Büyükhatipoğlu, S. ve Holtz, W. 1977. Preservation of trout sperm in liqiud of frozen state. Aquaculture, 14, 96-56.
  • Canyurt, M.A., Akhan, S. 2008. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences, 8, 171-175.
  • Ciereszko, A., Dabrowski, K. 1993. Estimation of spermdensity of rainbow sperctrophotometric technique. Aquaculture, 109, 367-373. whitefish and yellow perch using a
  • Cloud, J.G., Miller, W.H., Levanduski, M.J. 1990. Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Prog. Fish Cult. 52, 51–53.
  • DeGraaf, J.D., Berlinsky, D.L. 2004. Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234(1–4), 527–540.
  • Geffen, A.J., Evans, J.P. 2000. Sperm traits and fertilization success of male and sex reversed female rain bow trout (Oncorhynchus mykiss). Aquaculture, 182, 61-72.
  • Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski, H., Ciereszko, A. 2000. Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Research, 31, 289-296.
  • Hatipoğlu, T., Akçay, E. 2010. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniv Vet Fak Derg, 57, 33-38.
  • Linhart, O., Rodina, M., Gela, D., Kocour, M. 2004. Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture, 235, 619–632.
  • Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T. 2006. Cryopreservation of chinese sturgeon (Acipenser sinensis) sperm. J. Appl. Ichthyol. 22(supp.1), 384-388.
  • Munkıttrıck, K.R., Moccıa, R.D. 1987. Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen, J. and Hyvarinen, H. 1983. Composition of milt of some teleost fish. Journal of Fish Biology, 22, 351-361.
  • Rurangwa, E., Kime, D.E., Ollevier, F., Nash, J.P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
  • Saad, A., Billard, R., Theron, M.C. and Hollebecq, M.G. 1988. Short- therm presevation of carp (Cyprinus carpio) semen. Aquaculture, 71, 133-150.
  • Suquet, M., Billard, R., Cosson, J., Dorange, G., Chauvaud, L., Mugnier, C., Fauvel, C. 1994. Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species. Aquatic Living Resources, 7, 283-294.
  • Suquet, M., Dreanno, C., Dorange, G., Normant, Y., Quemener, L., Gaignon, J.L., Billard, R. 1998. The aging phenomenon of turbot (Scophthalmus maximus) spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization and storage capacities. Journal of Fish Biology, 32, 31-41.
  • Şahin, T., Kurtoğlu, İ.Z. ve Köse, Ö. 2013. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın spermatolojik özellikleri ve spermanın kısa süreli muhafazası. Fırat Unv. Journal of Science, 25(1), 77-92.
  • Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y., Kayam, S. 2003. Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Türk J Vet Anim Sci, 27, 37–44.
  • Tuset, V.M., Dietrich, G.J., Wojtczak, M., Słowińska, M., de Monserrat, J., Ciereszko, A. 2008. Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa. J. Appl. Ichthyol, 24, 393–397.
  • DeGraaf, J.D., King, V.W., Benton, C., Berlinsky, D.L. 2004. Production and storage of sperm from the black sea bass Centropristis striata. Aquacult. Res., 35, 1457-1465.
There are 31 citations in total.

Details

Primary Language Turkish
Journal Section Makaleler
Authors

Temel Şahin

Özay Köse

Publication Date June 25, 2015
Published in Issue Year 2015 Volume: 8 Issue: 1

Cite

APA Şahin, T., & Köse, Ö. (2015). KAYNAK ALABALIĞI (SALVELINUS FONTINALIS)’NIN SPERMATOLOJİK ÖZELLİKLERİ VE SPERMANIN KISA SÜRELİ MUHAFAZASI. Erzincan University Journal of Science and Technology, 8(1), 88-99. https://doi.org/10.18185/eufbed.90287

Cited By

Spermatologic characteristics and sperm motility alterations caused by short-term copper exposure in Brook Trout Salvelinus fontinalis
Toxin Reviews
Filiz Kutluyer
https://doi.org/10.1080/15569543.2018.1528466
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