Salvia microstegia Merikarplarının Antimikrobiyal ve Antibiyofilm Aktiviteleri Üzerine Bir Araştırma

Year 2022, Volume: 15 Issue: 2, 649 - 658, 31.08.2022

Zehra Öksüz Sevda Güzel Ahmet Kahraman

https://doi.org/10.18185/erzifbed.1136242

Abstract

Biyofilm ilişkili enfeksiyonlar tüm dünyada önemli sağlık sorunlarına neden olmakatadır. Antimikrobiyallere karşı gelişen direnç ve olası yan etkiler nedeniyle, araştırmacıların dikkati bitki ekstreleri ve onların bileşenleri üzerine yoğunlaşmıştır. Bu çalışmanın amacı Salvia microstegia Boiss. et Bal. merikarplarının antimikrobiyal aktivite ve antibiyofilm potansiyellerini değerlendirmektir. Merikarplardan elde edilen etanol ekstresinin antimikrobiyal aktivitesi yedi standart bakteri ve üç standart maya suşuna karşı mikrodilüsyon yöntemi kullanılarak belirlenmiştir. Ayrıca ekstrenin Pseudomonas aeruginosa’nın biyofilm oluşumunu inhibe etme ve oluşmuş biyofilmi üzerine etkisi kristal viyole yöntemi kullanılarak belirlenmiştir. Kullanılan ekstre, 62.5–250 µg/mL aralıkta minimum inhibisyon konsantrasyonu (MİK) değerleri ile test edilen bakteri ve mayaları karşı orta ve düşük antimikrobiyal aktivite göstermiştir. Bununla birlikte, ekstrenin önceden oluşturulmuş biyofilmleri inhibe etme potansiyelinin biyofilm oluşumunu engelleme potansiyelinden daha iyi olduğu tespit edilmiştir. Sonuç olarak, Salvia microstegia merikarplarından elde edilen ekstrenin antibiyofilm potansiyeli bulunmakla beraber, antimikrobiyal potansiyelinin daha geniş çerçevede ortaya konabilmesi için farklı suşların dâhil edildiği ilave çalışmalara ihtiyaç vardır.

Keywords

Salvia microstegia, merikarp, etanol ekstresi, antimikrobiyal, antibiyofilm

Study on Antimicrobial and Antibiofilm Activities of Salvia microstegia mericarps from Turkish flora

Abstract

Abstract
Biofilm-associated infections cause important health problems all over the world. Because of the resistance to antimicrobials and their possible side effects, researchers' attention has been focused on plant extracts and their components. The aim of this study is to evaluate the antimicrobial and antibiofilm potentials of Salvia microstegia Boiss. et Bal. mericarps. The antimicrobial activity of ethanolic extract obtained from the mericarps was evaluated using the microdilution method against seven standard bacteria and three standard yeast strains. In addition, the effect of the extract on inhibiting biofilm formation and preformed biofilm of Pseudomonas aeruginosa was determined using the crystal violet method. The used extract showed moderate to low antimicrobial activity against tested bacteria and yeasts with minimum inhibitory concentration (MIC) values ranging from 62.5 to 250 µg/mL. Moreover, the extract's potential to inhibit preformed biofilms was found to be greater compared to its potential to inhibit biofilm formation. In conclusion, the tested extract obtained from Salvia microstegia mericarps showed antibiofilm potential, however, additional studies involving different strains are needed to reveal its antimicrobial potential in a more holistic perspective.

Keywords

Salvia microstegia, mericarp, ethanol extract, antimicrobial, antibiofilm

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