DOĞU AKDENİZ SEDİMANLARINDAN İZOLE EDİLEN BAKTERİLERİN FİLOGENETİK ÇEŞİTLİLİĞİ VE ANTİBİYOTİK DİRENÇLİLİĞİ

Year 2019, Volume: 8 Issue: 2, 151 - 169, 31.07.2019

İlknur Tunçer , Nihayet Bizsel

https://doi.org/10.18036/estubtdc.598740

Abstract

Doğu
Akdeniz'in sığ ve derin bölgelerindeki sedimanlardan izole edilen bakterilerin
filogenetik çeşitliliği ve antibiyotik duyarlılığı çalışılmıştır. Dünyadaki en
oligotrofik ortamlardan biri olan çalışma alanından izole edilen 153 suşun Firmicutes ve Gammaproteobacteria taksonlarına ait oldukları bulunmuştur. 16S
rRNA gen dizisi analizi ile belirlendiği üzere, her sediman örneğinde Bacillus en sık görülen cins olmuştur.
Bu çalışmada 10 farklı bakteri ailesinden çok çeşitli türler elde edilmiş,
özellikle toplam izolatların yüzde 12’sini yeni türlerin oluşturduğu görülmüştür.
En yüksek antibiyotik direncinin derin havzalara kıyasla kıyı sedimanlarda
olduğu gösterilmiştir. Çevresel parametrelerin bakteri toplulukları üzerinde
etkisinin olduğu belirtilmekle birlikte Doğu Akdeniz'in derin havzalarına
kıyasla özellikle Kuzey Ege Denizi'ndeki daha sığ bölgeler için yüksek
taksonlarda daha fazla filogenetik çeşitliliğin olduğu ortaya konmuştur.

Keywords

Bakteri filogenetiği, 16S rRNA genleri, Antibiyotik duyarlılık, Akdeniz

PHYLOGENETIC DIVERSITY AND ANTIBIOTIC RESISTANCE OF BACTERIA ISOLATED FROM SEDIMENTS OF EASTERN MEDITERRANEAN SEA

Abstract

Phylogenetic diversity and antibiotic
susceptibility of isolated bacteria from sediments in shallow and deep regions of
Eastern Mediterranean Sea were studied. 153 strains isolated from the study
area which is one of the most oligotrophic environments in the world were found
as affiliated to the Firmicutes and Gammaproteobacteria. As determined by
16S rRNA gene sequence analysis, the Bacillus
the most frequent genus was dominant in each sediment samples. The present
study provided highly diverse bacterial strains of ten different families
especially with probability of representing new taxa as twelve percent of total
isolates. The highest antibiotic
resistance was obtained from coastal sediments compared to deep basins. The
influence of environmental parameters was observed as the variability of
bacterial community composition such that phylogenetic diversity was much
higher in higher taxa for shallower regions, especially in North Aegean Sea as
compared to deep basins of Eastern Mediterranean Sea.

Keywords

Bacterial phylogeny, 16S rRNA genes, Antibiotic susceptibility, Mediterranean Sea

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