Determination of Anther Culture Efficiency of Some Pepper Genotypes Originating from Kyrgyzstan

Abstract

Orta Asya ülkeleri arasında Kırgızistan biyolojik kaynakların zengin çeşitliliği bakımından önemli ülkelerden birisidir. Bu zengin çeşitliliğin arasında sebze ve meyve genetik kaynakları önemli bir yer tutmaktadır. Kırgızistan ekolojisiine benzer ülkelerde olduğu gibi Kırgızistan’da da ekonomik önem sahip ürünlerin arasında biber önemli bir yere sahiptir. Ülkemizde olduğu gibi Kırgızistan’ında biber gibi genetik kaynaklarının ıslah programlarına dahil edilmesi önemlidir. Islah programlarında genetik kaynaklarının double haploid etkinliklerinin bilinmesi avantaj sağlamaktadır. Buradan hareketle bu çalışmada Kırgızistan orijinli bazı biber genotiplerinin anter kültürü etkinliklerinin belirlenmesi amaçlanmıştır. Çalışmada Kırgızistan orijinli yirmi beş(25) adet ve kontrol olarak ise anter kültürü etkinliği bilinen iki(2) adet kapya tipinde genetik materyal olmak üzere toplam Yirmi yedi(27) adet biber genotipinin anter kültürü etkinliği belirlenmiştir. Elde edilen bulgulara göre toplam 1336 tomurcuk kültüre alınmış ve 365 adet embriyo elde edilirken bu embriyolardan 264 adeti sağlıklı bitkiye dönüşmüştür. En yüksek bitkiye dönüşüm BY-25 nolu genotipte belirlenmiştir. Yedi(7) adet biber genotipinde bitki elde edilmezken sadece yirmi(20) adet genotipte sağlıklı bitki elde edilmiştir. Tomurcuk başına embriyo oranı %14 olurken tomurcuk başına bitkiye dönüşüm oranı 15,6 olarak belirlenmiştir. Elde edilen bulgular, diğer genotiplerde olduğu gibi Kırgızistan orijinli biber genotiplerde de anter kültürü etkinliği bakımından varyasyonun olduğunu göstermiştir.

Keywords

• anther culture
• Pepper
• Kyrzistan

Kırgızistan Orijinli Bazı Biber Genotiplerinin Anter Kültürü Etkinliklerinin Belirlenmesi

Abstract

Among the Central Asian countries, Kyrgyzstan is one of the important countries in terms of the rich diversity of biological resources. Among this rich diversity, vegetable and fruit genetic resources have an important place. Pepper has an important place among economically important products in Kyrgyzstan, as in countries similar to Kyrgyzstan's ecology. It is important to include genetic resources such as pepper in breeding programs in Kyrgyzstan, as in our country. Knowing the double haploid efficiency of genetic resources is advantageous in breeding programs. In this study, it was aimed to determine the anther culture efficiency of pepper genotypes originating from Kyrgyzstan. In the study, the anther culture efficiency of a total of 27 pepper genotypes, 25 from Kyrgyzstan and 2 capia type genetic materials with known anther culture efficiency as controls, was determined. According to the findings, a total of 1336 buds were cultured and 365 embryos were obtained, while 264 of these embryos turned into healthy plants. The highest plant transformation was determined in genotype BY-25. While no plants were obtained in 7 pepper genotypes, healthy plants were obtained in only 20 genotypes. While the embryo rate per bud was 14%, the conversion rate to plant per bud was determined as 15.6. The results showed that there was variation in terms of anther culture efficiency in pepper genotypes originating from Kyrgyzstan, as in other genotypes.

Keywords

• Capsicum annum
• Kyrgizistan
• anther culture

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