Çerezlik Karpuz Genotiplerinin ISSR Tekniği ile Moleküler Karakterizasyonu

Year 2023, Volume: 6 Issue: 1, 51 - 58, 31.03.2023

Seher Toprak , Ömer Faruk Coşkun , Kazım Mavi

https://doi.org/10.55257/ethabd.1247106

Cited By: 2

Abstract

Bazı karpuz genotipleri tohum özelliklerinden dolayı çerezlik olarak yetiştirilmekte ve tüketilmektedir. Çerezlik potansiyeli olan genotiplerde ıslah çalışmalarının yapılabilmesi için genetik analizlerin gerçekleştirilmesi gerekmektedir. Bu çalışmada bazı çerezlik karpuz genotiplerinin genetik çeşitlilik ve populasyon yapısının belirlenmesi amaçlanmıştır. ISSR (Inter Simple Sequence Repeat) markır tekniği kullanılarak 24 genotipte 179 bant elde edilmiş ve polimorfizm oranı % 58.2 olarak hesaplanmıştır. Benzerlik katsayı değerlerinin 0.75-0.98 arasında olduğu belirlenmiştir. Kümeleme analizlerinde dört ana küme meydana gelmiştir. Structure analizlerinde genotiplerin iki alt populasyondan oluştuğu tespit edilmiştir. Çerezlik karpuz genotiplerinin ISSR tekniği ile genetik olarak ayırt edilebildiği, ancak genetik varyasyonun düşük olduğu sonucuna varılmıştır. Bu çalışma sonuçları çerezlik karpuz çeşitlerinin iyileştirilmesi için ıslah stratejilerinde kullanılabilir.

Keywords

Citrillus lanatus, ISSR, genetik çeşitlilik, populasyon yapısı

Supporting Institution

Hatay Mustafa Kemal Üniversitesi

Project Number

21.GAP.019

Thanks

Bu çalışmayı 21.GAP.019 kodlu proje ile destekleyen Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü’ne teşekkür ederiz.

Molecular Characterization of Edible Watermelon Genotypes by ISSR Technique

Abstract

Some watermelon genotypes are grown and consumed as a edible due to their seed characteristics. Genetic analyzes should be carried out in order to carry out breeding studies in genotypes with edible potential. In this study, it was aimed to determine the genetic diversity and population structure of some edible watermelon genotypes. Using the ISSR (Inter Simple Sequence Repeat) marker technique, 179 bands were obtained in 24 genotypes and the polymorphism rate was calculated as 58.2%. It was determined that the similarity coefficient values were between 0.75-0.98. In the cluster analysis, four main clusters were formed. Structure analysis revealed that genotypes consisted of two subpopulations. It was concluded that the edible watermelon genotypes could be distinguished genetically by the ISSR technique, but the genetic variation was low. The results of this study can be used in breeding strategies for the improvement of the edible watermelon cultivars.

Keywords

Citrillus lanatus, ISSR, genetic diversity, structure

Project Number

21.GAP.019

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