Potasyum Klorür ve Kalsiyum Sülfatın Daphnia magna (Crustacea: Cladocera) Üzerine Etkileri

Öz

Daphnia magna uygun ortam ve sıcaklıkta (15-22 °C) hızla çoğalabilir. Ayrıca D. magna’nın laboratuvarda üremesi son derece basit ve ucuzdur. Dahası su pireleri laboratuvar çalışmalarında sıklıkla kullanılan deney hayvanlarıdır. Bu nedenle bu çalışmada iki farklı bileşiğin (KCl ve CaSO4) D. magna'nın yumurta üretimi ve kalp hızı üzerine etkileri incelendi. Uygulama gruplarındaki su pireleri KCl ve CaSO4'ün farklı konsantrasyonlarına (37.5, 75, 150 mg/L) maruz bırakıldı. Her grupta on birey kullanıldı. Tüm deneysel örneklemeler üç tekrar halinde yapıldı. Kalp hızındaki değişiklikler 96 saat boyunca incelendi ve bir video kamera kullanılarak kalp atışları sayıldı. Her deney grubundaki birey başına düşen yumurta sayısı 21 gün boyunca kaydedildi. CaSO4'e maruz kalmanın bir sonucu olarak, konsantrasyon derecelerine bağlı olarak hem kalp hızında hem de yumurta üremesinde bir artış gözlendi. Ancak KCl'ye maruz kalma, orta ve yüksek doz gruplarında yumurta üretiminde azalmaya neden oldu. Ayrıca KCl'ye maruz kaldıktan sonra art arda iki gün boyunca kalp hızında bir artış incelenmiş olmasına rağmen, orta ve yüksek doz gruplarında kalp hızında son 48 saat içinde bir azalma gözlendi.

Anahtar Kelimeler

• Daphnia
• KCl
• CaSO4
• kalp
• yumurta

The effects of Potassium Chloride and Calcium Sulphate on Daphnia magna (Crustacea: Cladocera)

Abstract

Daphnia magna can reproduce rapidly in suitable environment and temperature (15-22 °C). Additionally, D. magna reproduction in laboratory is extremely simple and inexpensive. Moreover, daphnids are frequently used experimental animals in laboratory studies. Therefore, the effects of two different compounds (KCl and CaSO4) on egg production and heart rate in D. magna were examined in this study. Daphnids of treatment groups were exposed to different concentration of KCl and CaSO4 (37.5, 75, 150 mg/L). Ten individuals were used in each group. All experimental samples were made in triplicate. Changes in heart rate were examined for 96 h and the number of heart beats was counted by using a video camera. The number of eggs per individual in each experimental group was recorded for 21 days. As a result of the exposure to CaSO4, an increase in both heart rate and egg reproduction depend on concentration gradients were observed. However, exposure to KCl caused a decrease in the egg production of medium and high-dose groups. Additionally, although an increase in heart rate was examined for two consecutive days after exposure to KCl, a decrease in the heart rate of medium and high-dose groups was observed during the last 48 h.

Keywords

• Daphnia
• KCl
• CaSO4
• heart
• egg

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