Potasyum Klorür ve Kalsiyum Sülfatın Daphnia magna (Crustacea: Cladocera) Üzerine Etkileri

Öz

Daphnia magna uygun ortam ve sıcaklıkta (15-22 °C) hızla çoğalabilir. Ayrıca D. magna’nın laboratuvarda üremesi son derece basit ve ucuzdur. Dahası su pireleri laboratuvar çalışmalarında sıklıkla kullanılan deney hayvanlarıdır. Bu nedenle bu çalışmada iki farklı bileşiğin (KCl ve CaSO4) D. magna'nın yumurta üretimi ve kalp hızı üzerine etkileri incelendi. Uygulama gruplarındaki su pireleri KCl ve CaSO4'ün farklı konsantrasyonlarına (37.5, 75, 150 mg/L) maruz bırakıldı. Her grupta on birey kullanıldı. Tüm deneysel örneklemeler üç tekrar halinde yapıldı. Kalp hızındaki değişiklikler 96 saat boyunca incelendi ve bir video kamera kullanılarak kalp atışları sayıldı. Her deney grubundaki birey başına düşen yumurta sayısı 21 gün boyunca kaydedildi. CaSO4'e maruz kalmanın bir sonucu olarak, konsantrasyon derecelerine bağlı olarak hem kalp hızında hem de yumurta üremesinde bir artış gözlendi. Ancak KCl'ye maruz kalma, orta ve yüksek doz gruplarında yumurta üretiminde azalmaya neden oldu. Ayrıca KCl'ye maruz kaldıktan sonra art arda iki gün boyunca kalp hızında bir artış incelenmiş olmasına rağmen, orta ve yüksek doz gruplarında kalp hızında son 48 saat içinde bir azalma gözlendi.

Anahtar Kelimeler

• Daphnia
• KCl
• CaSO4
• kalp
• yumurta

The effects of Potassium Chloride and Calcium Sulphate on Daphnia magna (Crustacea: Cladocera)

Abstract

Daphnia magna can reproduce rapidly in suitable environment and temperature (15-22 °C). Additionally, D. magna reproduction in laboratory is extremely simple and inexpensive. Moreover, daphnids are frequently used experimental animals in laboratory studies. Therefore, the effects of two different compounds (KCl and CaSO4) on egg production and heart rate in D. magna were examined in this study. Daphnids of treatment groups were exposed to different concentration of KCl and CaSO4 (37.5, 75, 150 mg/L). Ten individuals were used in each group. All experimental samples were made in triplicate. Changes in heart rate were examined for 96 h and the number of heart beats was counted by using a video camera. The number of eggs per individual in each experimental group was recorded for 21 days. As a result of the exposure to CaSO4, an increase in both heart rate and egg reproduction depend on concentration gradients were observed. However, exposure to KCl caused a decrease in the egg production of medium and high-dose groups. Additionally, although an increase in heart rate was examined for two consecutive days after exposure to KCl, a decrease in the heart rate of medium and high-dose groups was observed during the last 48 h.

Keywords

• Daphnia
• KCl
• CaSO4
• heart
• egg

Kaynakça

1. [1] D. Ebert, “Ecology, epidemiology, and evolution of parasitism in Daphnia,” National Library of Medicine (US) and National Center for Biotechnology Information (US), Bethesda. ISBN10: 1-932811-06-0, 2005.
2. [2] S. Savaş and N. L. Çiçek. “Canlı yem organizmalarında L-karnitin,” Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 41, no. 1, pp. 71–73, 2010.
3. [3] S. Arman, E. Akat and S. İ. Üçüncü, “An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna Crustacea, Cladocera,” Hacettepe Journal of Biology and Chemistry, vol. 44, no. 2, pp. 155–160, 2016.
4. [4] X. Lv, Y. Yang, Y. Tao, Y. Jiang, B. Chen, X. Zhu, Z. Cai, and B. Li, “A mechanism study on toxicity of graphene oxide to Daphnia magna: Direct link between bioaccumulation and oxidative stress,” Environmental Pollution, vol. 234, pp. 953–959, 2018.
5. [5] E. Decaestecker, L. De Meester and J. Mergeay, “Cyclical parthenogenesis in Daphnia: sexual versus asexual reproduction,” In: Van Dijk P, Martens K, Schön I (eds) Lost Sex. Springer, Berlin, pp. 295–316, 2009.
6. [6] S. Navis, A. Waterkeyn, T. Voet, L. De Meester and L. Brendonck, “Pesticide exposure impacts not only hatching of dormant eggs, but also hatchling survival and performance in the water flea Daphnia magna,” Ecotoxicology, vol. 22, no. 5, pp. 803–814, 2013.
7. [7] A. A. Camp, M. H. Haeba, and G. A. LeBlanc, “Complementary roles of photoperiod and temperature in environmental sex determination in Daphnia spp,” Journal of Experimental Biology, vol. 222, no. 4, jeb195289, 2019.
8. [8] S. Burkhard, V. Van Eif, L. Garric, V. M. Christoffels and J. Bakkers, “On the evolution of the cardiac pacemaker,” Journal of Cardiovascular Development and Disease, vol. 4, no. 4, pp. 1–18, 2017.

9. [9] T. J. Pirtle, T. L. Carr, T. Khurana and G. Meeker, “ZD7288 and mibefradil inhibit the myogenic heartbeat in Daphnia magna indicating its dependency on HCN and T-type calcium ion channels,” Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, vol. 222, pp. 36–42, 2018.
10. [10] M. J. Davis and M. A. Hill, “Signaling mechanisms underlying the vascular myogenic response,” Physiological Reviews, vol. 79, pp. 387–423, 1999.
11. [11] I. M. Cooke, “Reliable, responsive pacemaking and pattern generation with minimal cell numbers: the crustacean cardiac ganglion,” Biology Bulletin, vol. 202, pp. 108–36, 2002.
12. [12] J. L. Wilkens, “Evolution of the cardiovascular system in Crustacea,” American Zoologist, vol. 39, no. 2, pp. 199–214, 1999.
13. [13] R. Liang, J. He, Y. Shi, Z. Li, S. Sarvajayakesavalu, Y. Baninla, F. Guo, J. Chen, X. Xu, L. Yu, “Effects of Perfluorooctane sulfonate on immobilization, heartbeat, reproductive and biochemical performance of Daphnia magna,” Chemosphere, vol. 168, pp. 1613–1618, 2017.
14. [14] W. S. Pietrzak and R. Ronk, “Calcium sulfate bone void filler: a review and a look ahead,” The Journal of Craniofacial Surgery, vol. 11, no. 4, pp. 327–333, 2000.
15. [15] H. Kahraman and C. C. Karaderi, “The Importance of KCl,” Biomedical Journal of Scientific & Technical Research, vol. 19 no. 2, pp. 14170–14171, 2019.
16. [16] A. Bownik, M. Jasieczek and E. Kosztowny, “Ketoprofen affects swimming behavior and impairs physiological endpoints of Daphnia magna,” Science of The Total Environment, vol. 725, 138312, 2020.
17. [17] A. Villegas-Navarro, E. Rosas-L and J. L. Reyes, “The heart of Daphnia magna: effects of four cardioactive drugs,” Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, vol. 136, no. 2 pp. 127–134, 2003.
18. [18] G. Cohlmia, D. Honarchi, K. Klaiber, E. May and S. Reeves, “Chemical control: observing the excitatory effects of MSG compared to the inhibitory effects of KCl in the heart rate of Daphnia magna,” Journal of Undergraduate Biology Laboratory Investigations, vol. 2, no. 2, pp. 1–5, 2019.
19. [19] K. O. Soetan, C. O. Olaiya and O. E. Oyewole, “The importance of mineral elements for humans, domestic animals and plants-A review,” African Journal of Food Science, vol. 4, no. 5, 200–222, 2010.
20. [20] A. Mattu, W. J. Brady and D. A. Robinson, “Electrocardiographic manifestations of hyperkalemia,” The American Journal of Emergency Medicine, vol. 18, no. 6, pp. 721–729, 2000.
21. [21] J. Wilson, J. Collette, A. Miller, A. Hoang and T. Dubose, “The opposite effects of caffeine and ethanol on Daphnia magna’s heart rate,” Journal of Undergraduate Biology Laboratory Investigations, vol. 2, no. 2, pp. 1–4, 2019.
22. [22] G. A. Ahearn, P. K. Mandal and A. Mandal “Calcium regulation in crustaceans during the molt cycle: a review and update,” Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, vol. 137, no. (2), pp. 247–257, 2004.
23. [23] C. Romero, E. M. Onyango, W. Powers, R. Angel and T. J. Applegate, “Effect of a partial replacement of limestone by a CaSO4-zeolite mixture combined with a slight protein reduction on production indices, egg quality, and excreta pH in laying hens,”. Journal of Applied Poultry Research, vol. 21, no. 2, pp. 325–334, 2012.
24. [24] A. Bhattacharya and B. B. Kaliwal, “The biochemical effects of potassium chloride on the silkworm, (Bombyx mori L.),” Insect Science, vol. 12, no. 2, pp. 95–100, 2005.
25. [25] S. N. Thompson, “Trehalose—the insect ‘blood’ sugar,” In: Advances in insect physiology, vol 31. Elsevier, Amsterdam, pp. 205–285, 2003.
26. [26] T. Yazawa, K. Tanaka and T. Katsuyama, “Neurodynamical control of the heart of healthy and dying crustacean animals,” In International Design Engineering Technical Conferences and Computers and Information in Engineering Conference 47438, pp. 1123–1130, 2005.
27. [27] D. A. Omana and J. Wu, “Effect of different concentrations of calcium chloride and potassium chloride on egg white proteins during isoelectric precipitation of ovomucin,” Poultry Science, vol. 88, no. 10 pp. 2224–2234, 2009.