ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF CARNOSIC ACID ON HUMAN LIVER CANCER CELLS

Abstract

Bu çalışmanın amacı, karnosikasitin tek başına ve sisplatin ile kombinasyonu insan karaciğer kanseri hücreleri üzerinde sitotoksik etkilerini ve bunların hidrojen peroksit varlığında veya yokluğunda indüklenen reaktif oksijen türlerini temizleme kapasitelerini araştırmaktır. Maddelerin insan karaciğer kanser hücreleri üzerindeki 24 ve 48 saatlik sitotoksik etkileri metil-tiyazol tetrazolyum-bromür testi ile değerlendirildi. Mitokondriyal membran potansiyeli JC-1 kiti ile tespit edildi. Hücre içi reaktif oksijen türlerinin düzeyleri 2’-7'diklorofloresin diasetat yöntemi kullanılarak belirlendi. Bulgularımıza göre, 24 ve 48 saatlik maruziyette karnosikasitin hem tek başına hem de sisplatin ile kombinasyonu insan karaciğer kanser hücrelerinde sitotoksik etkiler gösterdi. Özellikle, 48 saatlik maruziyette doza bağlı bir şekilde hücre canlılığını önemli ölçüde azalttığı görüldü ve kombine tedavinin daha belirgin bir sitotoksik etkiye sahip olduğu bulundu. Ayrıca, tüm karnosik asit konsantrasyonlarının tek başına ve sisplatin ile kombinasyonlarının mitokondriyal membran potansiyelini önemli ölçüde azalttığı belirlendi. Hem karnosikasitin tek başına hem de sisplatin ile kombinasyonu hidrojen peroksit varlığında veya yokluğunda hücre içi reaktif oksijen türlerinin düzeylerini düşürdüğünü gözlemledik. Sonuçlar, karnosikasitin tek başına veya sisplatin ile kombinasyon şeklinde karaciğer kanserinin tedavisinde umut verici bir ajan olabileceğini düşündürdü.

Keywords

• Antioksidanlar
• karnosik asit
• sisplatin
• HepG2
• reaktif oksijen türleri

Project Number

TYL-2018-8632

ANTIPROLIFERATIVE AND ANTIOXIDANT EFFECTS OF CARNOSIC ACID ON HUMAN LIVER CANCER CELLS

Abstract

The purpose of the study was to investigate the cytotoxic effects of carnosic acid alone and in combination with cisplatin on human liver cancer cells and their capacity to scavenge reactive oxygen species induced in the presence or absence of hydrogen peroxide.Cytotoxic effects of agents on human liver cancer cells for 24 and 48 hours were evaluated by methyl-thiazol tetrazolium-bromide assay. Mitochondrial membrane potential were detected JC-1 kit. The intracellular reactive oxygen species levels were determined using 2’-7’dichlorofluorescin diacetateassay. According to our findings, both carnosic acid alone and in combination with cisplatin showed cytotoxic effects in human liver cancer cells at 24 and 48 hours of exposure. In particular, it was seen that the cell viability significantly decreased in a dose-dependent manner at 48 hours of exposure, and the combined treatment was found to have a more pronounced cytotoxic effect. In addition, all carnosic acid concentrations alone and in combination with cisplatin were identified to significantly reduce mitochondrial membrane potential. We observed that both carnosic acid alone and in combination with cisplatin lowered intracellular reactive oxygen species levels in the presence or absence of hydrogen peroxide. The results suggested that carnosic acid alone or in combination with cisplatin might be a promising agent in the treatment of liver cancer.

Keywords

• Antioxidants
• carnosic acid
• cisplatin
• HepG2
• reactive oxygen species

Supporting Institution

Erciyes University

Project Number

TYL-2018-8632

Thanks

This study was funded by the Erciyes University Scientific Research Projects Unit with the project number TYL-2018-8632

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