Protective Effects of Octreotide on Acetaminophen-Induced Liver Damage in Rats: Biochemical and Histopathological Evaluation

Abstract

This study aimed to evaluate the potential protective effects of octreotide, a somatostatin analogue, against acute liver injury induced by acetaminophen in rats. 28 male Wistar albino rats, aged 8–10 weeks, were randomly assigned into four experimental groups. Group 1 served as the control, and Group 2 received 1 g/kg of acetaminophen orally, Group 3 was administered 300 µg/kg of octreotide intraperitoneally, Group 4 was treated with both agents, where octreotide was given 30 minutes after acetaminophen administration. The experiment was terminated by taking liver tissue and blood samples under general anesthesia 24 hours after drug administration. Hematoxylin and Eosin staining was performed on the liver sections, and serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, as well as total antioxidant capacity, total oxidant capacity and malondialdehyde levelswere analyzed. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, and total oxidant capacity were significantly higher in acetaminophen-treated rats compared to the other groups. Similarly, malondialdehyde levels were also higher, but these values were not statistically significant. Total antioxidant capacity were lower in the acetaminophen group compared to the other groups. In contrast, the treatment group showed a significant reduction in aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities and total oxidant capacitycompared to the acetaminophen group. Histological analysis revealed severe hepatic damage in the acetaminophen group, while less tissue damage was noted in the treatment group. Although malondialdehyde was lower in the treatment group on average, the difference was not statistically significant. These findings suggest that octreotide may offer partial protection against acetaminophen-induced liver injury. However, further investigation is required to elucidate its precise mechanism of action.

Keywords

• Acetaminophen
• liver injury
• octreotide
• oxidative stress

Sıçanlarda Asetaminofen Kaynaklı Karaciğer Hasarına Oktreotidin Koruyucu Etkileri: Biyokimyasal ve Histopatolojik Değerlendirme

Abstract

Bu araştırmanın amacı, somatostatin analoğu olan oktreotidin sıçanlarda asetaminofenle oluşturulan akut karaciğer hasarına karşı olası koruyucu etkilerini değerlendirmektir. Çalışmada, 8–10 haftalık toplam 28 yetişkin erkek Wistar albino sıçan rastgele dört gruba ayrıldı. Grup 1 kontrol olarak kullanıldı, Grup 2'ye 1 g/kg oral asetaminofen verildi, Grup 3'e 300 µg/kg intraperitoneal oktreotid uygulandı, Grup 4 her iki ajanla tedavi edildi, oktreotid asetaminofen uygulamasından 30 dakika sonra verildi. İlaç uygulamasından 24 saat sonra genel anestezi altında kan ve karaciğer dokusu örnekleri alındı. Karaciğer dokuları Hematoksilen-Eozin ile boyandı; serum örneklerinden aspartat aminotransferaz, alanin aminotransferaz, alkalin fosfataz, total antioksidan kapasite, total oksidan kapasitesi ve malondialdehid düzeyleri ölçüldü. Asetaminofen uygulanan sıçanlarda aspartat aminotransferaz, alanin aminotransferaz, alkalen fosfataz aktiviteleri ve toplam oksidan kapasitesi diğer gruplara göre anlamlı derecede yüksekti. Benzer şekilde, malondialdehit seviyeleri de daha yüksekti, ancak bu değerler istatistiksel olarak anlamlı değildi. Toplam antioksidan kapasite değerleri ise diğer guplara göre asetaminofen grubunda düşüktü. Tedavi grubunda ise aspartat aminotransferaz, alanin aminotransferaz, alkalin fosfataz ve total oksidan kapasitesi, yalnızca asetaminofen verilen gruba kıyasla anlamlı şekilde düşüktü. Histopatolojik değerlendirmede asetaminofen grubunda ciddi doku hasarı gözlenirken, tedavi grubunda daha az hasar tespit edildi. Malondialdehid düzeyleri tedavi grubunda daha düşük seyretse de bu fark istatistiksel olarak anlam kazanmadı. Elde edilen bulgular, oktreotidin asetaminofen kaynaklı karaciğer hasarını kısmen hafifletebileceğini göstermektedir. Ancak bu etkinin mekanizmasının daha iyi anlaşılabilmesi için ileri çalışmalara ihtiyaç vardır.

Keywords

• Asetaminofen
• karaciğer hasarı
• oktreotid
• oksidatif stres

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