CYP2C9 VE VKORC1 GENLERİNİN PROSTAT KANSERİ ETYOLOJİSİNDEKİ ROLLERİNİN ARAŞTIRILMASI

Year 2019, , 75 - 80, 20.09.2019

Ali Osman Arslan , Selma Düzenli

https://doi.org/10.34108/eujhs.449023

Abstract

1.
Amaç: Çalışmamızda, Sitokrom p450 enzimlerini kodlayan CYP
ve vitamin K epoksit redüktaz kompleks subünit1 enzimini kodlayan VKORC1
genlerinin alt gruplarındaki tek nükleotit polimorfizmlerinin muhtemel bireysel
ve ortak etkilerini prostat kanseri hastalarında araştırmayı amaçladık. Çalışma
sonuçları aynı zamanda bu iki gen ile ilgili henüz ortaya konmamış olan bizim
populasyonumuza özgü genotip dağılımını da kısmen ortaya koyacaktır.                 

2. Gereç ve Yöntem: Çalışmamıza
48 prostat kanseri tanılı erkek hasta ve 48 sağlıklı erkek birey dâhil edildi. Hasta grubu prostat tanısı almış
yaş aralığı 49-86 olan gönüllü bireylerden seçildi. Kontrol grubu prostat kanseri
olmadığı belirlenmiş, 51-86 yaş grubundaki gönüllü bireylerden seçildi. Hasta
ve kontrol grubu bireylerinin venöz kanları alınarak genomik DNA izolasyonları
yapıldı. İzole edilen DNA’ların ilgili gen bölgeleri polimeraz zincir
reaksiyonu (PCR) ile çoğaltıldı. Sonrasında PCR ürünleri mikroarray
cihazında özel
problar yardımıyla çalışıldı. CYP2C9 ve VKORC1 gen bölgelerine ait alt
grupların polimorfizmleri tanımlandı ve EpiInfo 3.5.1 istatistik programı ile
anlamlılıkları hesaplandı.          

3. Bulgular: Sonuçlarımızda
CYP2C9 ve
VKORC1 enzimlerini kodlayan genlerin alt gruplarının polimorfizmleri karşılaştırıldığında
hasta ve kontrol grubu arasında yüzde olarak önemli farklar görüldü. Örneğin
VKORC1 6853 G>C polimorfizmi için kontrol grubunda CC alleli %25 iken hasta
grubunda %34,5 olarak tespit edildi. CYP2C9 *3 A>C değişimi için kontrol
grubunda CC alleli %79,16 iken hasta grubunda %87,5 olarak tespit edildi.

4. Sonuç: Çalışmamızda
sayı ve yüzde olarak hasta ve kontrol grubunda
önemli farklar görüldü. CYP2C9 ve VKORC1 geninin alt gruplarının analizleri
sonucunda istatistiksel anlamlılık tespit edilmedi. Bunun sebebinin örnek
sayısı azlığı ile alakalı olduğu düşünüldü ve ileri çalışmaların konuyu
aydınlatacağı düşünüldü.

Keywords

VKORC1, CYP2C9, Sitokrom p450, Prostat, kanser

The Role of Genes CYP2C9 and VKORC1 in the Ethiology of Prostate Cancer

Abstract

1. Objective: In
our study, we aimed to investigate possible individual and common effects of
single nucleotide polymorphisms of the gene encoding the enzyme cytochrome p450
(CYP) and the gene encoding the enzyme vitamine K epoxide reductase complex
subunit 1 (VKORC1) and their subgroups in patients with prostate cancer. The
results of the study also will partially reveal the distribution of genotypes
specific to our population, which have not yet been addressed in relation to
these two genes.

2. Methods: 48
prostate cancer diagnosed patients and 48 healty male individuals were included
in our study. The patient group was selected from volunteers with a
prostate-diagnosed age range of 49-86. The control group was selected among
without prostate cancer and aged 51-86 years individual. Venous blood was taken
from all individuals. Targeted regions were amplified by polymerase chain
reaction (PCR) from isolated DNAs. PCR products were genotyped using microarray
with appropriate DNA probes. Polymorphisms in the subgroups of the CYP2C9 and
VKORC1 gene regions were identified. The results were calculated using the
EpiInfo 3.5.1 statistical program.

3. Result: The
percentages between patient and control groups showed significant
differences when the subgroup polymorphisms of genes encoding CYP2C9 and VKORC1
enzymes were compared. For example, the CC allele of VKORC1 6853 G> C
polymorphism found to be %25 in the control and %34.5 in the patient group.
The
CC allele of CYP2C9 * 3 A> C change found %79.16 in the control and %87.5 in
the patient group.

4. Conclusion: Significant
differences were seen in number and percentage of patients and control groups
in our study. As a result of analysis of the subgroups of CYP2C9 gene and of the
VKORC1 gene, statistical significance was not observed. But the
percentage results show important differences, though we expect statistical
significiance when the numbers of individual is increased

Keywords

VKORC1, CYP2C9, Cytochrome p450, Prostate

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