EVALUATION OF THE CONDENSED CHROMATIN INDEX IN MITOGEN-STIMULATED LYMPHOCYTES PUVA TREATED PATIENTS

Abstract

UV-A ve psoralenlerle birlikte kullanılan fotokemoterapi (PUVA) vitiligo ve psoriasis gibi bazı deri hastalıklarında yaygın olarak kullanılan bir tedavidir. PUVA tedavisi aynı zamanda çeşitli hücre tiplerinde apoptosis ve kromatin yoğunlaşmasına neden olmaktadır. Bu çalışmanın amacı PUVA ile tedavi edilen hastaların mitojenle uyarılmış lenfositlerinde yoğunlaşmış kromatin indeksinin (YKi) , kardeş kromatid değişim (KKD) ve mikronukleus (MN) metodları gibi DNA hasarlarının belirlenmesinde bir test olarak k u l l a n ı l ı p k u l l a n ı l m a y a c a ğ ı n ı t e s p i t etmektir.Çekirdekteki kromatin yoğunluğunu ölçmek için özel bir bilgisayar programı yapıldı. Çalışmaya PUVA tedavisi alan 20 hastanın mitojenle uyarılmış lenfositleri ile başlandı. Her hastanın sıfırıncı saatte (n=20) ve tedavinin 20’ci (n=16), 40’ıncı (n=12) ve 60’ıncı (n=10) seans sonrası, mitojenle uyarılmış 100 interfaz çekirdeği YKi ortalaması değerlendirildi. Bu seanslar sırasıyla 57.81 ± 23.85, 181.43 ± 46.42, 299.56 ± 61.03 jul/cm2 UVA and 0.6x20, 0.6x40, 0.6x60 mg/kg methoxypsoralen (8-MOP)’e karşılık gelmektedir. Tedavi öncesi (sıfirıncı saat) ile 20. ve 40. seans sonrası elde edilen YK değerleri arasında bir fark bulunamamıştır ( p> 0.05 ). Bununla birlikte , tedavi öncesi ile 60. seans sonrası arasında istatistiksel olarak önemli bir fark bulunmuştur ( p<0.05 ). YKi ‘de duyarlılık, yaklaşık 300 jul/cm2 UVA ve 0.6x20, 0.6x40, 0.6x60 mg/kg 8-MOP’a karşılık gelen PUVA tedavisinin ortalarından sonra başlamaktadır

Keywords

• PUVA, Yoğunlaşmış kromatin, bilgisayarla analiz

PUVA Tedavisi Gören Hastaların Mitojenle Uyarılmış Lenfositlerindeki Yoğunlaşmış Kromatin İndeksinin Değerlendirilmesi

Abstract

Photochemotherapy with psoralens and UV-A (PUVA) is a treatment used widely in some skin disease like as vitiligo and psoriasis. PUVA treatment induces also chromatin condensation and apoptosis in several cell types. The aim of this study is to assay if the condensed chromatin index (CCi) in stimulated lymphocytes of PUVA treated patients may be used as a test method for the detection of DNA damage like the micronucleus (MN) and the sister chromatid exchange (SCE) methods. A special image analysis procedure has been designed to evaluate CCi in the nucleus. The work was initiated with the Mitogen- stimulated lymphocytes of 20 PUVA treated patients. The mean CCi (CCmi) have been evaluated at zero time (n=20) and after 20 (n=16), 40 (n=12), 60 (n=10) sessions of PUVA treatment in 100 mitogen- stimulated interphase nuclei for each patient. These sessions correspond reciprocally to 57.67 ± 23.74, 181.43 ± 46.42 and 299.56 ± 61.03 joules/cm2 of UVA and 0.6x20, 0.6x40, 0.6x60 mg/kg of methoxypsoralen (8-MOP). There was no difference between CCmi obtained before treatment (zero time) and after 20, 40 sessions of PUVA treatment (p> 0.05). However, statistically significant difference was found between untreated and 60 sessions treated individuals (p<0.05). CCi sensitivity begins after the moderate term of PUVA treatment corresponding to nearly 300 Joules/cm2 of UVA and 0.6x60 mg/kg of 8MOP

Keywords

• PUVA, Condensed chromatin, image analysis

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