KAYSERİ’DE TÜKETİME SUNULAN KANATLI ETLERİNDEN ARCOBACTER SPP’NİN İZOLASYONU

Abstract

Bu araştırmada, Kayseri ilindeki farklı satış noktalarından alınan tavuk, hindi ve bıldırcın karkaslarında Arcobacter spp.’nin varlığı araştırıldı. Tavuk karkas numunelerini bütün tavuk, but, göğüs, kanat ve baget; hindi numunelerini göğüs ve kanat oluştururken; bıldırcın numuneleri ise bütün olarak çalışmada kullanıldı. Bu amaçla, Kayseri ilindeki 5 marketten toplam 50 adet tavuk, 50 adet hindi ve 50 adet bıldırcın karkas örneği toplandı. Numunelerin her biri steril distile suda yıkandıktan sonra Arcobacter Enrichment Broth (AEB) içerisinde mikroaerobik koşullarda 30 oC’de kültüre edildi. Daha sonra kanlı agar (KA) üzerine yerleştirilmiş 0.45 µm selüloz asetat membran filtreler kullanılarak membran filtrasyon tekniği uygulandı. Araştırmada, bütün tavuk numunesinde 5/9 (% 25), but numunesinde 4/10 (% 20), göğüs numunesinde 5/9 (% 25), kanat numunesinde 2/10 (% 10), baget numunesinde 4/12 (% 20); hindilerden ise kanat numunelerinden 1/25 (% 4) ve göğüs numunelerinden 1/25 (% 4) ve bütün olarak incelenen bıldırcın numunelerinden 4/50 (% 8) Arcobacter spp. izole edildi. Sonuç olarak; Kayseri ilinde bulunan marketlerde satışa sunulan taze kanatlı karkaslarının genellikle Arcobacter spp.’lerle kontamine olduğu saptandı. İnsanlarda başta enterit olmak üzere diğer hastalık olgularından da izole edildikleri göz önünde bulundurulduğunda etkenin halk sağlığı açısından önemli bir risk faktörü oluşturabileceği sonucuna varıldı

Keywords

• Arcobacter spp., tavuk, hindi, bıldırcın, izolasyon

Isolation of Arcobacter spp. From Poultry Carcasses Put on Consumption in Kayseri

Abstract

In this study, the presence of Arcobacter spp. was examinated in carcasses of chicken, turkey and quail sold different markets in Kayseri. While carcasses samples of chickens included whole chicken rump, breast, wing and little rump; samples of turkeys included breast and wing; samples of quail was used as a whole in this study. For this aim; 50 chickens, 50 turkeys and 50 quails carcasses samples were collected from 5 markets in Kayseri. After each sample was washed in sterile distilled water and inoculated at 30°C under microaerobic conditions in Arcobacter Enrichment Broth (AEB). Membrane filtration technique was applied to washing liquid by using cellulose acetate membrane filter which has 0.45 µm pore diamater and was put on blood agar (BA). In this research, 5/9 (% 25) chickens samples, 4/10 (% 20) rumps samples, 5/9 (% 25) breast samples, 2/10 (% 10) wings samples, 4/12 (% 20) drumstick samples and from turkeys 1/25 (% 4) wings samples and 1/25 (% 4) breast samples and 4/50 (% 8) quail samples which is investigated as a whole Arcobacter spp. was isolated. The study revealed that fresh poultry carcasses was contaminated with Arcobacter spp. Sold in Kayseri when considered that Arcobacter spp. Was isolated from many disease in addition to human enteritis. It was concluded that Arcobacter spp. Might be an important risk factor as far as public health is concerned

Keywords

• Arcobacter spp., chicken, Turkey, quail, isolation

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