ÇEŞİTLİ BİTKİ TÜRLERİNDE VİTAMİN B12, FOLİK ASİT VE BİOTİN İÇERİĞİNİN İMMUNO-ENZİMATİK YÖNTEMLE ARAŞTIRILMASI

Abstract

Bu çalışmada, çeşitli bitki türlerinde folik asit, biotin ve vitamin B, içeriğinin immuno enzimatik yöntemle belirlenmesi amaçlanmıştır. Ispanak, pırasa, marul, maydanoz, kekik, nane, sarımsak, iğde çiçeği, şalgam tohumu ve kuşburnu örnekleri, Kayseri merkezindeki yerel pazarlardan taze ve temiz olarak temin edildikten sonra 5 gr bitki örneği distile su ve 1ml alfa amilaz ilavesinden sonra inkubasyona tabi tutulmuş ve kaynatılmıştır. Carrez I ve Carrez II çözeltilerinin ilavesinden sonra santrifüj edilmiştir. Süzüntü sulandırılmış, bu çözeltiden ve standart çözeltilerinden kuyucuklara konulmuştur. Sırasıyla enzim kongujat, substrat ve kromojen ilave edilerek bekletilmiş, reaksiyonu sonlandırma çözeltisi ilave edildikten sonra ELISA cihazında 450 nm de ölçüm yapılmış ve sonuçlar ppb olarak elde edilmiştir. Folik asit düzeyleri ppb olarak sırasıyla ıspanakta 0.698, marulda 0.711, maydanozda 3.450, kuşburnunda 2.139, şalgam tohumunda 0.959, iğde çiçeğinde 10.303 bulunmuştur. Vitamin B12 düzeyleri ppb olarak sırasıyla ıspanakta 11.070, nanede 90.088, kekikte 168.330, sarımsakta 10.744, kuşburnunda 21.240, iğde çiçeğinde 44.127 bulunmuştur. Biotin düzeyleri ppb olarak sırasıyla ıspanakta 249.436, pırasada 330.228, marulda 630.676, maydanozda 273.521, nanede 278.973, sarımsakta 942.248, kuşburnunda 589.469 bulunmuştur. Biotinin en fazla sarımsak ve marulda, B12 vitamininin en fazla kekikte, folik asidin en fazla iğde çiçeğinde olduğu tespit edilmiştir

Keywords

• Biotin, bitki, ELISA, folik asit, vitamin B12

The Investigation of Vitamin B12, Folic Acid and Biotin Content of Different Plant Species by Enzyme Immuno Assay

Abstract

The aim of this study is to determine vitamin B12, folic acid, and biotin content of different plant species by enzyme- immuno assay. Spinach, leek, lettuce, parsley, thyme, mint, garlic, oleaster, turnip seed, rosehip samples were obtained from the regional markets of Kayseri and used fresh and clean in the study. 5-10 g of plant sample was added to distilled water and 1 ml of alpha amylase solution and then incubated and boiled. After the addition of Carrez I and Carrez II solutions sample solutıon was centrifuged. The supernatant was diluted, each standard solution or prepared sample was added to seperate wells and then enzyme conjugate, substrate and chromogen solutions were respectively added to each well, incubated at room temperature. After the addition of stop solution. Absorbance was measured at 450 nm by ELISA instrument. Results obtained as ppb. The levels of folic acid as ppb were 0.698 in spinach, 0.771 in lettuce, 3.450 in parsley, 2.139 in rosehip, 0.959 in turnip seed, 10.303 in oleaster found. The levels of vitamin B12 as ppb were 11.070 in spinach, 90.088 in mint, kekikte 168.330, 10.744 in garlic, 21.240 in rosehip, 44.127 in oleaster. The levels of biotin as ppb were 249.436 in spinach, 330.228 in leek, 630.676 in lettuce, 273.521 in parsley, 168.330 in thyme, 278.973 in mint, 942.248 in garlic, 589.469 in rosehip. The highest biotin content was found in garlic and lettuce. The highest vitamin B12 content was found in thyme. Oleaster has the highest folic acid content

Keywords

• Biotin, ELISA, folic acid, plant, vitamin B12

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