THE EFFECTIVENESS OF SYNCHRONIZATION OF ESTRUS VIA PROSTAGLANDIN F2α ADMINISTRATION AND TIMED ARTIFICIAL INSEMINATON IN MULTIPAR AND NULLIPAR AKKARAMAN BREED EWES

Abstract

Sunulan çalışmada; prostaglandin F2α (PGF2α) ile östrus senkronizasyonu ve sabit zamanlı tohumlamanın multipar ve nullipar Akkaraman ırkı koyunlarda, etkinliğinin araştırılması amaçlandı. Araştırmada 10 adet multipar (2-4 yaşında, Grup 1) ve 10 adet nullipar koyun (1,5-2 yaşında, Grup 2) olmak üzere iki grupta toplam 20 adet hayvan kullanıldı. Tüm hayvanlara 9 gün arayla 125 µg Cloprostenol (Estrumate® DİF İstanbul Türkiye) enjekte edildi. Sperma altı adet koçtan (2-3 yaşında) suni vagen yardımı ile alındı ve 150 milyon/0,5 ml dozda sulandırıldı. Koyunlar ikinci PGF2α uygulamasından 42 saat sonra intravaginal yolla tohumlandı. Kandaki progesteron düzeyleri hormon enjeksiyonları anında ve 3’er gün sonrasında değerlendirildi. Gebelikler, tohumlamadan 35 gün sonra, ultrasonografi ile teşhis edildi. Gebelik oranları multipar koyunlarda % 40, nulliparlarda % 20 olarak belirlendi (p>0.05). Birinci PGF2α uygulaması öncesinde, birinci PGF2αuygulamasından üç gün sonra, ikinci PGF2αuygulamasından önce ve ikinci PGF2α uygulamasından üç gün sonra multipar-nullipar koyunlarda ortalama progesteron düzeyleri sırasıyla, 2.67-3.67 ng/ml (p>0.05), 0.96-0.86 ng/ml (p>0.05), 6.26-5.24 ng/ml (p<0.05) ve 0.99-1.25 ng/ml (p<0.05) ölçüldü. Sonuç olarak, multipar hayvanların PGF2α uygulaması ile yapılan östrus senkronizasyonuna daha iyi cevap verdiği kanaatine varıldı. Nullipar hayvanlar, koyunlarda PGF2α ile östrus senkronizasyonu ve sabit zamanlı tohumlamaların etkisiz kalmasında, bir faktör olarak değerlendirilmelidir

Keywords

• Akkaraman koyunu, multipar, nullipar, östrus senkronizasyonu, PGF2α, sabit zamanlı tohumlama

Multipar ve Nullipar Akkaraman Irkı Koyunlarda Prostaglandin F2α ile Östrus Senkronizasyonu ve Sabit Zamanlı Tohumlamanın Etkinliği

Abstract

In present study it was aimed to investigate the effectiveness of synchronization of estrus via prostaglandin F2α(PGF2α) administration and timed artificial insemination (TAI) in multipar and nullipar Akkaraman breed sheep. The study used a total of 20 animals which were divided into two groups: Group 1=10 multipar ewes (2-4 years old) and Group 2=10 nullipar ewes (1.5-2 years old). All animals were injected with 125µg Cloprostenol (Estrumate® DIF İstanbul, TURKEY) at nine day intervals. Ewes were inseminated vaginally 42 hours after the second PGF2α administration with the semen which were collected from six purebred Akkaraman rams (2-3 years old) and diluted at 150x 10motile spermatozoa/0.5 ml dose. Progesterone levels in blood samples were assessed at the moment of hormone injections and after three days. Thirty five days after the inseminations, pregnancies were diagnosed via ultrasonography. Pregnancy rates were found to be 40 % for multipar and 20 % for nullipar ewes (p>0.05). Progesterone levels in multipar and nullipar animals at the first PGF2α administration, three days after the first PGF2αadministration, at the second PGF2α administration, and three days after the second PGF2α administration were measured as (median) 2.67-3.67 ng/ml (p>0.05), 0.96-0.86 ng/ml(p>0.05), 6.26-5.24 ng/ml (p<0.05) and 0.99-1.25 ng/ml (p<0.05) respectively. It was concluded that multipar animals yielded better responses to ES accomplished through PGF2α administration. The nullipar animals should be considered as a factor for effectivenessless of timed artificial insemination via synchronization of estrus with PGF2α in ewes

Keywords

• Akkaraman ewes, multiparous, nulliparous, estrus synchronization, PGF2α, timed artificial insemination

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