VARİKOSELLİ HASTALARDA SPERM MORFOLOJİSİNİN DEĞERLENDİRİLMESİ

Abstract

Pampiniform pleksustaki testiküler venlerin anormal genişlemesi olarak tanımlanan varikosel çoğunlukla, etkilenen testisin hacmindeki bir azalma ile ilişkilidir. Varikosel erkek infertilitesinin önemli nedenlerinden biridir ve fertilitede ilerleyen bir azalmaya sebep olur. Bu çalışmanın amacı, varikoselli hastalarda sperm ve semen parametrelerini değerlendirerek semen kalitesi ve sperm morfolojisini belirlemektir.Varikosel tanısı konmuş toplam 52 hastadan semen örnekleri elde edildi. Semen örnekleri 3 günlük cinsel perhizden sonra masturbasyonla toplandı. Likefaksiyondan sonra, semen analizi Dünya Sağlık Örgütü (WHO) kriterlerine göre Makler kamera ile yerine getirildi. Sperm morfolojisi Diff-Quick boyama tekniği kullanılarak değerlendirildi. Bu çalışmada hastaların A+B hareketli sperm oranı ortalaması %30.8’di. A+B hareketli sperm oranı %50’nin altında olan hasta sayısı 49 (%94.3) olup A hareketli spermlerinin ortalaması %10.9 olarak bulundu. Hastaların B hareketli spermlerinin ortalaması %19.9 olarak bulundu. 52 hastanın 44 ‘ünde (%84.7) normal sperm morfoloji oranı %30’un altında idi. Tüm hastalarda normal sperm morfoloji ortalaması ise %13.7 olarak bulundu. Baş, boyun ve kuyruk anomalileri sırasıyla %61.8, %15 ve %8.3’tü. 52 hastanın yaşı 21 ile 38 arasında (ortalama 30) değişmekteydi. Yaş ile toplam motilite (p>0.05) ve yaş ile toplam anormal sperm morfolojisi arasında (p>0.05) korelasyon bulunmadı. Sonuç olarak, varikoselin sperm yapısı ve fonksiyonu üzerinde negatif bir etkiye sahip olduğu belirlendi

Keywords

• Varikosel, spermiogram, Sperm morfolojisi

Evaluation of Sperm Morphology in Patients with Varicocele

Abstract

Varicocele, defined as an abnormal dilation of the testicular veins in the pampiniform plexus, is often associated with a reduction in the volume of the affected testicle. Varicocele is an important cause of male infertility and it causes a progressive decrease in fertility status. The aim of this study is to examine the semen quality and sperm morphology in patients with varicocele using sperm and semen parameters. Semen samples were obtained from a total of 52 patients diagnosed with varicocele. Semen samples were collected by masturbation after 3 days of sexual abstinence. After semen liquefaction, seminal analysis was performed using Macler camera according to WHO criteria, and sperm morphology was evaluated by using Diff-Quick staining technique. In this study, the A+B motile sperm ratio average of the patients was 30.8%. The number of patients whose motile sperm ratio was below 50% is 49 (93.4%), and the average of the A-motile sperms proved to be 10.9%,. The average of B-motile sperms of the patients was discovered 19.9%. In 44 of the 52 patients (84.7%), the sperm morphology ratio was below 30%. In all the patients, the normal sperm morphology mean was 13.7%. The head, neck and tail anomalies were 61.8%, 15%, and 8.3% successively. The ages of the 52 patients varied between 21 and 38 years (with an average of 30). No correlation between age and total motility (p>0.05) and age and total abnormal sperm morphology (p>0.05) has been discovered. In conclusion, it is understood that it has got a negative effect on the varicocele sperm structure and function

Keywords

• Varicocele, spermiogram, sperm morphology

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