The In vitro Effects of Propolis Extracts Prepared with Different Solvents on Mutans Streptococci

Abstract

Propolis is a resinous substance made by bees. It possesses many biological activities, and many studies have reported its potential application in the control of dental caries. The aim of this study was to evaluate in vitro antimicrobial effects of Turkish propolis on Streptococcus mutans from mutans streptococci.Propolis samples was serially fractionated into ethanol, methanol, chloroform, hexane, ethyl acetate and propylen glicol. Then, solutions were removed by evaporation and propolis extracts were obtained except propylen glycol which has high evaporation temperature. Ethanol, methanol, choroform, hexane, ethly acetate extracts were used after 80% of ethanol dilution and propolis + propylen glycol was used directly. In vitro antimicrobial activity of these extracts on Streptococcus mutans was examined by determining minimum inhibitory consentration (MIC) and minimum bactericidal consentration (MBC) As a result, the methanol and ethyl acetate extracts showed the highest antimicrobial activity on Streptococcus mutans

Keywords

• Antimicrobial activity, caries, propolis, streptococus mutans

FARKLI ÇÖZÜCÜLERLE HAZIRLANAN PROPOLİS ÖZÜTLERİNİN MUTANS STREPTOKOKLAR ÜZERİNE IN VITRO ANTİMİKROBİYAL ETKİSİ

Abstract

Propolis arılar tarafından üretilen reçine içeren bir maddedir. Çok sayıda biyolojik aktiviteye sahip olan propolisin diş çürüğünün kontrolünde potansiyel uygulamalarıyla ilgili araştırmalar yapılmaktadır. Bu çalışmada, Türk propolisinin mutans streptokoklardan Streptococcus mutans üzerine antimikrobiyal etkisini değerlendirmek amaçlanmıştır.Propolis örnekleri etanol, metanol, kloroform, hekzan, etil asetat, ve propilen glikol ile fraksiyonlarına ayrılmıştır. Propilen glikolün buharlaşma ısısı yüksek olduğu için Propilen glikol dışındaki uzaklaştırılmış ve propolis özütleri elde edilmiştir. Etanol, metanol, kloroform, hekzan, etil asetat özütleri % 80 etanolde çözülerek, propolis + propilen glikol karışımı ise direkt kullanılmıştır. Bu özütlerin Streptococcus mutans üzerine in vitro antimikrobiyal konsantrasyon (MIC) ve minimum bakterisidal konsantrasyon belirlenmiştir. Sonuç olarak, Streptococcus mutans üzerine en iyi inhibitör etkiyi propolisin metanol ve etil setat özütleri göstermiştir

Keywords

• Antimikrobiyal aktivite, çürük, propolis, streptococus mutans

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