TANACETUM ZAHLBRUCKNERI (NÁB.) GRIERSON’ NUN SEKONDER METABOLİTLERİ ve ANTİOKSİDAN ETKİLERİ

Abstract

Bu çalışma kapsamında Asteraceae familyasına dahil endemik bir tür olan Tanacetum zahlbruckneri (Náb.) Grierson bitkisinin toprak üstü kısımlarından hazırlanan metanol ekstresinin DPPH● , ABTS●+ radikallerini süpürücü aktivitesi ve β-karoten-linoleik asitle oksidasyon yöntemi ile antioksidan aktiviteleri, Folin– Ciocalteu yöntemiyle toplam fenol, flavonit ve flavonol içeriği incelenmiştir. Metanol ekstresinden sıvı-sıvı partisyon yöntemiyle n-hekzan, dietil eter, diklorometan ve n- butanol ekstreleri hazırlanmış, n-butanol ekstresinden çeşitli kromatografik yöntemler kullanılarak, klorojenik asit (1), rutin (2) ve konduritol F (3) izole edilmiştir. Elde edilen bileşiklerin yapıları spektroskopik yöntemler (IR, 1H-NMR, 13C-NMR, 2D-NMR, IR, MS) kullanılarak aydınlatılmıştır. Bu çalışma ile Konduritol F Tanacetum türlerinden ilk defa izole edilmiştir. Klorojenik asit ve rutinin bitkinin metanol ekstresindeki kantitatif tayini Yüksek Basınçlı Sıvı Kromatografisi ile gerçekleştirilmiştir. İzole edilen bileşiklerin antioksidan aktiviteleri ölçülmüş ve klorojenik asitin aktivitesi, rutin ve metanol ekstresinden daha yüksek bulunmuştur.

Keywords

• Tanacetum zahlbruckneri, Klorojenik asit, Rutin, Konduritol F, HPLC

Secondary Metabolites and Antioxidant Activities of Tanacetum Zahlbruckneri(Náb.)Grierson

Abstract

In the current study, antioxidant activity of methanol extract prepared from Tanacetum zahlbruckneri (Asteraceae) was studied with DPPH●, ABTS+ radical scavenging and beta-carotene-linoleic acid cooxidation assays. Total phenol, flavonoid and flavonol contents of extracts were determined with Folin- Ciocalteu method. n-Hexane, diethyl ether, dichloromethane and n-butanol extracts were prepared from methanol extract with solvent partitioning and chlorogenic acid, rutin and conduritol F were isolated from n-BuOH extract with various chromatographic methods. Structures of the compounds were elucidated by extensive 1D- and 2D- NMR spectroscopic analysis in combination with IR, MS experiments. Conduritol F was isolated for the first time from Tanacetum species. Quantitative analyses of chlorogenic acid and rutin in MEOH extract were determined with High Pressure Liquid Chromatography (HPLC). Antioxidant activities of isolated compounds were studied and chlorogenic acid was found to be more active than rutin and methanol extract.

Keywords

• Tanacetum zahlbruckneri, Chlorogenic acid, Rutin, Conduritol F, HPLC

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