ANTIMICROBIAL ACTIVITY AND THE PHENOLIC PROFILE OF FIVE SCROPHULARIA L. SPECIE

Abstract

Scrophularia L. genus (Scrophulariaceae) has 310 taxa worldwide. Some Scrophularia species are used in folk medicine for the treatment of different skin inflammatory diseases and as antibacterial. The aim of the study was to investigate the antimicrobial activity and determine the phenolic profile of S. kotscyhana Bentham, S. cinarescens Boiss., S. catariifolia Boiss. & Heldr., S. chrysantha Jaub&Spach and S. scopolii var. adenocalyx Hoppe. ex Pers. Phenolic components were analyzed by an HPLC-DAD method. Disk diffusion method and microtiter-plate assay were used to determine the antimicrobial activities of the aerial parts methanolic extracts of the species. Our results revealed that all of the tested species contain caffeic acid and ferulic acid in varied concentrations and none of them have chlorogenic acid. All of the plant species exhibited moderate activity against Candida albicans (MIC values; 0.3125 mg/mL). Besides, S. cinarescens exhibited moderate activity against Enterococcus faecalis (MIC value; 0.3125 mg/ mL) and S. chrysantha exhibited moderate activity against Pseudomonas aeruginosa (MIC value; 0.3125 mg/mL). The results of this study showed that further studies were needed to identify the secondary metabolites of the species and evaluate their antimicrobial activities.

Keywords

• Antimicrobial activity, HPLC-DAD, microtiter-plate assay, medicinal plants, Scrophularia species.

Beş Scrophularia L. Türünün Antimikrobiyal Aktivitesi Ve Fenolik Profili

Abstract

Scrophularia L. cinsi (Scrophulariaceae) dünya üzerinde 310 taksona sahiptir. Bazı Scrophularia türleri halk arasında çeşitli iltihaplı cilt hastalıklarının tedavisinde ve antibakteriyel olarak kullanılır. Çalışmanın amacı, S. kotscyhana Bentham, S. cinarescens Boiss., S. catariifolia Boiss. & Heldr., S. chrysantha Jaub&Spach ve S. scopolii var. adenocalyx Hoppe. ex Pers. türlerinin antimikrobiyal aktivitesini araştırmak ve fenolik profilini belirlemektir. Fenolik bileşenler bir HPLC-DAD yöntemi ile analiz edildi. Türlerin toprak üstü kısımlarının metanol ekstrelerinin antimikrobiyal aktivitelerini belirlemek için disk difüzyon yöntemi ve mikrodilüsyon yöntemi kullanıldı. Bulgularımız, test edilen tüm türlerin, çeşitli konsantrasyonlarda kafeik asit ve ferulik asit içerdiğini ve hiçbirinin klorojenik asit içermediğini ortaya koymuştur. Çalışılan tüm türler, Candida albicans'a (MIC değerleri; 0.3125 mg / mL) karşı orta derecede aktivite göstermiştir. Ayrıca, S. cinarescens, Enterococcus faecalis’e karşı (MIC değeri; 0.3125 mg/mL) ve S. chrysantha Pseudomonas aeruginosa’ya karşı (MIC değeri; 0.3125 mg/mL) orta derecede aktivite gösterdi. Bu çalışmanın sonuçları, türlerin sekonder metabolitlerinin aydınlatılması ve antimikrobiyal etkinliklerinin değerlendirilmesi için daha ileri çalışmalara ihtiyaç duyulduğunu gösterdi.

Keywords

• Antimikrobiyal aktivite, HPLCDAD, mikrodilüsyon yöntemi, tıbbi bitkiler, Scrophularia türleri

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