PARVOVİRUS İLE DOĞAL ENFEKTE KÖPEKLERDE MATRİKS METALLOPROTEİNAZ-9 EKSPRESYONU

Abstract

Köpek parvoviral enteritis 1978 yılında ortaya çıktığından bu zamana kadar genç köpeklerde yaygın olarak görülmektedir. Sindirim yolu ile alındıktan sonra sindirim kanalında ve kemik iliğinde mitotik aktivitesi yüksek hücreleri tercih ederek şiddetli kusma, ishal, ateş, dehidrasyon ve halsizliğe sebep olur. MMP’ler embriyonik gelişim, farklılaşma, proliferasyon ve doku rejenerasyonunda önemli rol oynarlar. Bununla birlikte MMP’leri aktive ve kontrol eden faktörler arasında bir dengesizlik oluştuğunda bağırsak yangısı, artrit, ateroskleroz veya kanser dahil bir çok hastalık ile sonuçlanır. Önemli olarak, MMP-9'un ekspresyon seviyesi bağırsak yangı modellerinde ve Crohn hastalığı veya ülseratif kolit gibi yangısal bağırsak hastalıkları olan insanlarda daha yüksektir. Bu çalışmanın amacı, MMP9'un parvovirus ile enfekte köpeklerin bağırsak enfeksiyonları üzerindeki rolünü araştırmaktır. Bu çalışmada, parvovirus ile doğal enfekte 21 köpek bağırsak dokusu MMP-9 antikoru ile boyandı ve beş adet normal sağlıklı köpek dokusu kontrol olarak kullanıldı. Sonuç olarak parvovirus ile enfekte köpeklerin bağırsak dokularından MMP-9 ekspresyonu gerçekleşirken kontrol dokularda ekspresyon gözlenmedi. Bu sonuçlar köpeklerde parvovirüs ile oluşan enteritisin patogenezinde MMP-9 ekspresyonunun etkisinin olabileceğini işaret etmektedir.

Keywords

• Parvoviral enteritis, MMP-9, köpek

Matrıx Metalloproteınase-9 Expressıon In Natural Infected Dogs Wıth Parvovırus

Abstract

Canine parvoviral enteritis has, since its emergence in 1978, been common in young dogs. After ingestion, the virus attacks rapidly dividing cells in the intestinal tract and bone marrow, causing severe vomiting, diarrhoea, fever, rapid dehydration, lethargy and decreased activity. MMPs are crucial for embryonic development, differentiation, proliferation, and regeneration of tissues. An imbalance between MMPs activating and inhibiting factors, however, results in a plethora of diseases including intestinal inflammation, arthritis, atherosclerosis or cancer. Importantly, expression levels of MMP-9 is upregulated in intestinal inflammation models and in patients suffering from human inflammatory bowel diseases such as Crohn’s disease or ulcerative colitis. The aim of this study is to investigate the role of MMP-9 in the intestinal infection of dogs with parvovirus. In this study, intestinal tissues of 21 dog naturally infected with parvovirus were stained with MMP-9 antibody and five normal healty dog tissues were used as control. As a result, MMP-9 expression from intestinal tissues of dogs with parvovirus-infected occurred while control tissues did not expression. These results indicate that MMP-9 expression might have an impact on the pathogenesis of parvovirus-induced enteritis in dogs.

Keywords

• Parvoviral enteritis, MMP-9, dog

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