TAVŞANLARDA FARKLI EIMERIA STIEDAI SUŞLARI İLE OLUŞTURULAN DENEYSEL KARACİĞER KOKSİDİYOZUNDA PATOLOJİK VE BİYOKİMYASAL BULGULARIN KARŞILAŞTIRILMASI

Abstract

Çalışma, tavşanlarda E. stiedae’nin saha ve standart suşuyla oluşturulan karaciğer koksidiyozisinde klinik, biyokimyasal ve patolojik bulguları karşılaştırmak amacıyla yapılmıştır. Çalışmada, 1-1.5 kg, 6-8 haftalık 36 adet yeni Zelanda tavşanı kullanılmıştır. Hayvanlar 3 gruba ayrılmıştır. Birinci grup kontrol tutulurken, sırasıyla ikinci ve üçüncü gruptaki tavşanlara, 1 ml’sinde 50.000 sporlanmış oosit içeren saha ve standart suştan, 1 ml direkt sonda ile ağızdan mideye verilmiştir. Makroskopik ve histolojik olarak Eimeria stiedae standart suşu inokule edilen üçüncü gruba göre, saha suşu inokule edilen ikinci grupta daha şiddetli karaciğer lezyonları belirlenmiştir. Etken inokulasyonunun 20. ve 30. günlerinde; serum gamma glutamiltransferaz (GGT), alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) enzim aktivitelerinde grup bir ile grup ikideki tavşanlar arasında istatistiksel önemli bir fark bulunmamıştır. Buna karşın, etken inokulasyonunun 20. ve 30. günlerinde, grup birdeki tavşanlara ait serum GGT, ALT ve AST enzim aktivitelerine göre grup üçteki tavşanların enzim aktivitelerinde önemli bir artış (p<0.001) belirlenmiştir. Tavşanlarda E. stiedae’nin saha ve standart suşu ile oluşturulan karaciğer koksidiyozisinde bazı klinik, biyokimyasal ve patolojik değişikliklerin, oluşan enfeksiyonun şiddeti ile farklılık gösterebileceğinin ortaya konulduğu ilk çalışmadır

Keywords

• Biyokimyasal parametre, Eimeria stiedae, patoloji, tavşan.

Comparison of Pathological and Biochemical Findings in Experimentally Induced the Liver Coccidiosis by Different Types of Eimeria Stiedae Strains in the Rabbits

Abstract

The aim of this study was to compare clinical, biochemical and pathological findings of the liver coccidiosis induced by field and standard strain of the Eimeria stiedae in the rabbits. Thirty-six New Zealand rabbits weighing 1.5 kg and aged 6-8 weeks were used. The rabbits were allocated into three groups, the first of which was healthy control group. One ml of inoculum of field strain and standard strain containing 50 000 sporulated E.stiedae oocysts per ml were administered orally by a catheter to each rabbit in the second group and third group, respectively. Liver lesions were more severe in the second group of the Eimeria stiedae field strain inoculated than the third group in which the Eimeria stiedae standard strain was inoculated macroscopically and histologically. No statistical difference was found in serum GGT, ALT and AST enzyme activities between the first and second group at day 20 and 30 after the inoculation. However, at day 20 and 30 after the inoculation, a significant increase (p<0.05) was found in serum GGT, ALT and AST enzyme activities of the rabbits in the third group compared to those in group one. This is the first study to suggest that some clinical, biochemical and pathological changes in the liver coccidiosis induced with a field and standard strain of E. stiedae may vary depending on the severity of the infection.

Keywords

• Biochemical parameter, Eimeria stiedae, pathology, rabbit.

References

1. 1. Karaer Z. Evcil Tavşanlarda Coccidiosis. In: Dinçer Ş (eds), Coccidiosis, Türkiye Parazitoloji Derneği Yayın, Bornova-İzmir 2001; ss 269-278.
2. 2. Silva SM, Ferreira C, Paupéri J, et al. Coccidiosis in European rabbit (Oryctolagus Cuniculus Algirus) populations in the Iberian peninsula. Acta Parasitologica 2015; 60: 350-355.
3. 3. Li H, Shen M, Hou Z, et al. Morphology and Molecular Identification of the Eimeria spp. in Domestic Rabbits. Pakistan Journal of Zoology 2016; 48:289-291.
4. 4. Duszynski DW, Couch L. The Biology and Identification of the Coccidia (apicomplexa) of Rabbits of the World. Elsevier, USA 2013; pp 122- 183.
5. 5. Soulsby EJL. Helmints, Arthropods and Protozoa of Domesticated Animals (7th Ed). BailliereTindall, London 1968; pp 149-190.
6. 6. Bhat TK, Jithendran KP, Kurade NP. Rabbit coccidiosis and its control: a review. World Rabbit Science 1996; 32:37-41.
7. 7. Pellérdy LP. Coccidia and Coccidiosis (2nd Ed). Verlag Paul Parey, Budapest 1974; pp 450-483.
8. 8. Polozowski A. Coccidiosis of rabbits and its control. Wiadomosci Parazytologiczne 1993; 39:13-28.

9. 9. El-Shahawi GA, El-Fayomi HM, Abdel-Haleem HM. Coccidiosis of domestic rabbit (Oryctolagus cuniculus) in Egypt: light microscopic study. Parasitology research 2012; 110:251-258.
10. 10. Li MH, Ooi HK. Fecal occult blood manifestation of intestinal Eimeria spp. infection in rabbit. Veterinary parasitology 2009; 161:327- 329.
11. 11. Oncel T, Gulegen E, Senlık B, ve ark. Intestinal coccidiosis in angora rabbits (Oryctolagus cuniculus) caused by Eimeria intestinalis, Eimeria perforans and Eimeria coecicola. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 2011; 22:27- 29.
12. 12. Levine ND, Ivens V. Coccidia of the Leporidae. The Journal of Protozoology 1972; 19:572-581.
13. 13. Al-Saeed MH, Al Saeed AH, Jori MM. Study of physiological and histological changes in rabbits induced with hepatic coccidiosis. Journal University of Kerbala 2017; 15:217-228.
14. 14. Hassan KM, Arafa WM, Mousa WM, et al. Molecular diagnosis of Eimeria stiedae in hepatic tissue of experimentally infected rabbits. Experimental parasitology 2016; 169:1-5.
15. 15. Arafa MA, Wanas MQ. The efficacy of ivermectin in treating rabbits experimentally infected with Eimeria as indicated parasitologically and histologically. Journal of the Egyptian Society of Parasitology 1996; 26:373-380.
16. 16. Baker DG. Flynn’s Parasitology of Laboratory Animals (2nd ed). Blackwell Publishing Company, Ames 2007; pp 451-501.
17. 17. Joyner LP, Catchpole J, Berrett S. Eimeria stiedae in rabbits. The demonstration of responses to chemotherapy. Research in Veterinary Science 1983; 34:64-67.
18. 18. El-Ghoneimy A, El-Shahawy I. Evaluation of amprolium and toltrazuril efficacy in controlling natural intestinal rabbit coccidiosis. Iranian journal of veterinary research 2017; 18:164-169.
19. 19. Sivajothi S, Reddy BS, Rayulu VC. Study on impression smears of hepatic coccidiosis in rabbits. Journal of Parasitic Diseases 2016; 40:906 -909.
20. 20. Al-Quraishy S, Metwaly MS, Dkhil MA et al. Liver response of rabbits to Eimeria coecicola infections. Parasitology Research 2012; 110:901- 911.
21. 21. Fairbanks VF, Klee GG. Biochemical aspect of haematology. Fundamentals of Clinical Chemistry, NW Tietz (Ed), Saunders, Philadelphia 1987; pp 803-804.
22. 22. Abdel-Ghaffar F, Marzouk M, Ashour MB, et al. Effects of Eimeria labbeana and E. stiedae infection on the activity of some enzymes in the serum and liver of their hosts. Parasitology research 1990; 76:440-443.
23. 23. Gomez-Bautista M, Garcia MV, Rojo-Vazquez FA. The levels of total protein fractions in the serum of rabbits infected with Eimeria stiedae. Annales De Parasitologie Humaine Et Comparee 1986; 61:393-400.
24. 24. Karademir B, Ersan Y, Koç E, ve ark. Tavşanlarda Doğal Bağırsak Koksidiyozunda Bazı Serum Enzim ve Mineral Madde Düzeyleri Üzerine Etkisi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 2011; 22:67-70.
25. 25. Özkan O, Sarı B, Bayezit M, ve ark. Evcil Tavşanlarda Thymus serpyllum’un Koksidiozise Karşı Etkisi: Oosit Atılımı ve Canlı Ağırlık Değişimi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2010; 16:323-327.
26. 26. Çam Y, Atasever A, Eraslan G, et al. Eimeria stiedae: experimental infection in rabbits and the effect of treatment with toltrazuril and ivermectin. Experimental parasitology 2008; 119:164-172.
27. 27. Ütük AE, Pişkin FÇ, Balkaya İ, et al. Molecular detection of Eimeria stiedae in an Angora rabbit. Etlik Veteriner Mikrobiyoloji Dergisi 2015; 26:41 -44.
28. 28. Levine ND, Ivens V. Coccidia of the Leporidae. The Journal of Protozoology 1972; 19: 572-581.
29. 29. Yakhchali M, Tehrani A. Eimeriidosis and pathological findings in New Zealand white rabbits. Journal of Biological Science 2007; 7:1488-1491.
30. 30. Singla LD, Juyal PD, Sandhu BS. Pathology and therapy in naturally Eimeria stiedae-infected rabbits. The Journal of Protozoology 2000; 4:185 -191.