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Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması

Year 2019, , 172 - 176, 30.12.2019
https://doi.org/10.34108/eujhs.567907

Abstract

Amaç: Civan
perçemi (CP) antioksidan, antibakteriyel, diüretik ve anti-inflamatuar etkilere
sahiptir. Çalışmamızda, streptozotosinle diyabet oluşturulan sıçan karaciğer
dokularında MDA seviyesi ve antioksidan enzim aktivitesi üzerine CP’nin koruyucu
etkisi araştırıldı.

Gereç ve Yöntem: Çalışmada
250-300 gr 40 adet erkek Wistar albino sıçanlar kullanıldı. Kontrol grubu,
Diyabet grubu ve CP ile tedavi edilen diyabet grubu olmak üzere 3 gruba
ayrıldı. Sıçanlarda diyabet oluşturmak için tek doz 60 mg/kg streptozotosin (STZ)
intraperitoneal olarak enjeksiyon yapıldı. Tedavi grubuna ise 6 hafta süre ile
250 mg/kg/gün CP verildi. Deneyin sonunda bütün hayvanlar sakrifiye edildi.

Bulgular:
Çalışma
sonucunda streptozotosinle diyabet oluşturulmuş sıçanlarda kontrol grubu ile
karşılaştırıldıklarında d
iyabet oluşturulan sıçanların karaciğer
dokusundaki lipit peroksidasyon ürününde (MDA) ve
SOD
enzim aktivitesinde,
CAT ve GSH düzeylerinde istatistiksel
olarak anlamlı derecede fark olduğu gözlendi (p<0.001.sırası ile). Bununla birlikte diyabetik sıçanlarda 6
hafta CP tedavisi sonrası, doku MDA seviyelerinde istatistiksel olarak anlamlı
bir azalma gözlenirken, GST ve CAT enzim aktivitelerinde anlamlı bir artış (p<0.001.sırası ile) olduğu gözlendi.







Sonuç:
Diyabette
oksidatif stresin indüklenmesinin önemli olduğu düşünülmektedir. Bu çalışmada CP’nin
antioksidan enzim aktivitelerinde artışa neden olduğundan diyabet durumunda lipid
peroksidasyonuna karşı koruyucu etkilerinin olabileceğini göstermektedir.

References

  • 1. Hosseini SE, Tavakoli F, Karami M. Medicinal Plants in the treatment of Diabetes mellitus. Clinical Excellence. 2014; 2: 64–89.
  • 2. J. Ghosh, J. Das, P. Manna, P.C. Sil, The protective role of arjunolic acidagainst doxorubicin induced intracellular ROS dependent JNK-p38and p53-mediated cardiac apoptosis, Biomaterials 2011; 32 (21): 4857–4866.
  • 3. J. Das, A. Roy, P.C. Sil, Mechanism of the protective action of tau-rine in toxin and drug induced organ pathophysiology and diabeticcomplications: a review, Food Funct 2012; 2 (12): 1251–1264.
  • 4. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J. 2012; 12: 5–18.
  • 5. Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Bravo SB. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med. 2017; 102: 133–148.
  • 6. N. E. Thomford, K. Dzobo, D. Chopera, A. Wonkam, M. Skelton, D. Blackhurst, S. Chirikure, C. Dandara. Pharmacogenomics implications of using herbal medicinal plants on african populations in health transition. Pharmaceuticals 2015; 8: 637-663.
  • 7. L. G. Ranilla, Y. I. Kwon, E. Apostolidis, K. Shetty. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol 2010; 101: 4676-4689.
  • 8. E. Koksal, H. Tohma, O. Kilic, Y. Alan, A. Aras, I. Gulcin, E. Bursal, Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: Analysis of its phenolic compounds using HPLC-MS/MS. Sci Pharm 2017; 85: 24.
  • 9. M. Kanter, Free radicals, exercise and antioxidant supplementation. Proc Nutr Soc 1998; 57: 9-13.
  • 10. N. Saeed, M. R. Khan, M. Shabbir, 'Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophyllaL', BMC Complement. Altern. Med. 2012; 12: 221.
  • 11. M. Villalva, L. Jaimea, D. Villanueva-Bermejo, B. Laraa, T. Fornaria, G. Regleroa, S. Santoyo. Supercritical anti-solvent fractionation for improving antioxidant and antiinflammatory activities of an Achillea millefolium L. extract. Food Research International 2019; 115: 128–134.
  • 12. Giorgi, A., Mingozzi, M., Madeo, M., Speranza, G., & Cocucci, M. Effect of nitrogen starvation on the phenolic metabolism and antioxidant properties of yarrow (Achillea collina, Becker ex Rchb.). Food Chemistry 2009; 114: 204–221.
  • 13. Tadíc V, Arsic I, Zvezdanovic J, Zugic A, Cvetkovic D, Pavkov S. (2017). The estimation of the traditionally used yarrow (Achillea millefolium L. Asteraceae) oil extarcts with anti-inflammatory potential in topical application. Journal of Ethnopharmacology 2017; 199: 138–148.
  • 14. Van Ye TM, Roza AM, Pieper GM, et al. Inhibition of intestinal lipid peroxidation does not minimize morphologic damage. J Surg Res 1993; 55(5): 553-8.15. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-6.
  • 16. Durak I, Canbolat O, Kavutcu M, Ozturk HS, Yurtarslani Z. Activities of total, cytoplasmic, and mitochondrial superoxide dismutase enzymes in sera and pleural fluids from patients with lung cancer. J Clin Lab Anal 1996; 10(1): 17-20.
  • 17. Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249(22): 7130-9.
  • 18. Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Bravo SB. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med 2017; 102: 133-148.
  • 19. Ois Moreau F, Pinget M, Maillard E, verine Sigrist S. Oxidative stress status and liver tissue defenses in diabetic rats during intensive subcutaneous insulin therapy. Exp Biol Med. 2015:1–9.
  • 20. Aboonabi A, Rahmat A, Othman F. Antioxidant effect of pomegranate against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats. Toxicol Rep. 2014; 1: 915–922.
  • 21. Tabei SM, Fakher S, Djalali M, Javanbakht MH, Zarei M,Derakhshanian H, Sadeghi MR, Mostafavi E, Kargar F. Effect of vitamins A, E, C and omega-3 fatty acids supplementation on the level of catalase and superoxide dismutase activities in streptozotocin-induced diabetic rats. Bratisl Lek Listy. 2015; 116(2): 115-8.
  • 22. Aluwong T, Ayo JO, Kpukple A, Oladipo OO. Amelioration of hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic Wistar rats treated with probiotic and vitamin C. Nutrients 2016; 8(5): 151.
  • 23. Garg MC, Chaudhary DP, Bansal DD. Effect of vitamin E supplementation on diabetes induced oxidative stress in experimental diabetes in rats. Indian J Exp Biol 2005; 43(2): 177-180.
  • 24. Allagui MS, Feriani A, Bouoni Z, Alimi H, Murat JC, El Feki A. Protective effects of vitamins (C and E) and melatonin co-administration on hematological and hepatic functions and oxidative stress in alloxan-induced diabetic rats. J Physiol Biochem 2014; 70(3): 713-723.
  • 25. Sofi I A, Gopalakrishnan B. and Venkatesalu V. Pharmacognosy, Phytochemistry and Pharmacological Properties of Achillea millefolium L.: A Review. Phytotherapy Research 2017; 31: 1140–1161.
  • 26. Kav S, Hanoğlu Z, Algıer L. Türkiye’de Kanserli Hastalarda Tamamlayıcı ve Alternatif Tedavi Yöntemlerinin Kullanımı: Literatür Taraması. Uluslararası hematoloji-Onkoloji Dergisi 2008; 18(1): 1.
  • 27. Yong LI, Zhang M L, Cong B at al. Achillinin A, a Cytotoxic Guaianolide from the Flower of Yarrow,Achillea millefolium. Biosci. Biotechnol. Biochem 2011; 75(8): 1554–1556.
  • 28. 19. Bafrani H H, Parsa Y, Damavandi S Y. Biochemical and Pathological Study of Hydroalcoholic Extract of Achillea millefolium L. on Ethylene Glycol-Induced Nephrolithiasis in Laboratory Rats. N Am J Med Sci. 2014; 6(12): 638–642.
Year 2019, , 172 - 176, 30.12.2019
https://doi.org/10.34108/eujhs.567907

Abstract

References

  • 1. Hosseini SE, Tavakoli F, Karami M. Medicinal Plants in the treatment of Diabetes mellitus. Clinical Excellence. 2014; 2: 64–89.
  • 2. J. Ghosh, J. Das, P. Manna, P.C. Sil, The protective role of arjunolic acidagainst doxorubicin induced intracellular ROS dependent JNK-p38and p53-mediated cardiac apoptosis, Biomaterials 2011; 32 (21): 4857–4866.
  • 3. J. Das, A. Roy, P.C. Sil, Mechanism of the protective action of tau-rine in toxin and drug induced organ pathophysiology and diabeticcomplications: a review, Food Funct 2012; 2 (12): 1251–1264.
  • 4. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J. 2012; 12: 5–18.
  • 5. Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Bravo SB. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med. 2017; 102: 133–148.
  • 6. N. E. Thomford, K. Dzobo, D. Chopera, A. Wonkam, M. Skelton, D. Blackhurst, S. Chirikure, C. Dandara. Pharmacogenomics implications of using herbal medicinal plants on african populations in health transition. Pharmaceuticals 2015; 8: 637-663.
  • 7. L. G. Ranilla, Y. I. Kwon, E. Apostolidis, K. Shetty. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol 2010; 101: 4676-4689.
  • 8. E. Koksal, H. Tohma, O. Kilic, Y. Alan, A. Aras, I. Gulcin, E. Bursal, Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: Analysis of its phenolic compounds using HPLC-MS/MS. Sci Pharm 2017; 85: 24.
  • 9. M. Kanter, Free radicals, exercise and antioxidant supplementation. Proc Nutr Soc 1998; 57: 9-13.
  • 10. N. Saeed, M. R. Khan, M. Shabbir, 'Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophyllaL', BMC Complement. Altern. Med. 2012; 12: 221.
  • 11. M. Villalva, L. Jaimea, D. Villanueva-Bermejo, B. Laraa, T. Fornaria, G. Regleroa, S. Santoyo. Supercritical anti-solvent fractionation for improving antioxidant and antiinflammatory activities of an Achillea millefolium L. extract. Food Research International 2019; 115: 128–134.
  • 12. Giorgi, A., Mingozzi, M., Madeo, M., Speranza, G., & Cocucci, M. Effect of nitrogen starvation on the phenolic metabolism and antioxidant properties of yarrow (Achillea collina, Becker ex Rchb.). Food Chemistry 2009; 114: 204–221.
  • 13. Tadíc V, Arsic I, Zvezdanovic J, Zugic A, Cvetkovic D, Pavkov S. (2017). The estimation of the traditionally used yarrow (Achillea millefolium L. Asteraceae) oil extarcts with anti-inflammatory potential in topical application. Journal of Ethnopharmacology 2017; 199: 138–148.
  • 14. Van Ye TM, Roza AM, Pieper GM, et al. Inhibition of intestinal lipid peroxidation does not minimize morphologic damage. J Surg Res 1993; 55(5): 553-8.15. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-6.
  • 16. Durak I, Canbolat O, Kavutcu M, Ozturk HS, Yurtarslani Z. Activities of total, cytoplasmic, and mitochondrial superoxide dismutase enzymes in sera and pleural fluids from patients with lung cancer. J Clin Lab Anal 1996; 10(1): 17-20.
  • 17. Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249(22): 7130-9.
  • 18. Patche J, Girard D, Catan A, Boyer F, Dobi A, Planesse C, Bravo SB. Diabetes-induced hepatic oxidative stress: a new pathogenic role for glycated albumin. Free Radic Biol Med 2017; 102: 133-148.
  • 19. Ois Moreau F, Pinget M, Maillard E, verine Sigrist S. Oxidative stress status and liver tissue defenses in diabetic rats during intensive subcutaneous insulin therapy. Exp Biol Med. 2015:1–9.
  • 20. Aboonabi A, Rahmat A, Othman F. Antioxidant effect of pomegranate against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats. Toxicol Rep. 2014; 1: 915–922.
  • 21. Tabei SM, Fakher S, Djalali M, Javanbakht MH, Zarei M,Derakhshanian H, Sadeghi MR, Mostafavi E, Kargar F. Effect of vitamins A, E, C and omega-3 fatty acids supplementation on the level of catalase and superoxide dismutase activities in streptozotocin-induced diabetic rats. Bratisl Lek Listy. 2015; 116(2): 115-8.
  • 22. Aluwong T, Ayo JO, Kpukple A, Oladipo OO. Amelioration of hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic Wistar rats treated with probiotic and vitamin C. Nutrients 2016; 8(5): 151.
  • 23. Garg MC, Chaudhary DP, Bansal DD. Effect of vitamin E supplementation on diabetes induced oxidative stress in experimental diabetes in rats. Indian J Exp Biol 2005; 43(2): 177-180.
  • 24. Allagui MS, Feriani A, Bouoni Z, Alimi H, Murat JC, El Feki A. Protective effects of vitamins (C and E) and melatonin co-administration on hematological and hepatic functions and oxidative stress in alloxan-induced diabetic rats. J Physiol Biochem 2014; 70(3): 713-723.
  • 25. Sofi I A, Gopalakrishnan B. and Venkatesalu V. Pharmacognosy, Phytochemistry and Pharmacological Properties of Achillea millefolium L.: A Review. Phytotherapy Research 2017; 31: 1140–1161.
  • 26. Kav S, Hanoğlu Z, Algıer L. Türkiye’de Kanserli Hastalarda Tamamlayıcı ve Alternatif Tedavi Yöntemlerinin Kullanımı: Literatür Taraması. Uluslararası hematoloji-Onkoloji Dergisi 2008; 18(1): 1.
  • 27. Yong LI, Zhang M L, Cong B at al. Achillinin A, a Cytotoxic Guaianolide from the Flower of Yarrow,Achillea millefolium. Biosci. Biotechnol. Biochem 2011; 75(8): 1554–1556.
  • 28. 19. Bafrani H H, Parsa Y, Damavandi S Y. Biochemical and Pathological Study of Hydroalcoholic Extract of Achillea millefolium L. on Ethylene Glycol-Induced Nephrolithiasis in Laboratory Rats. N Am J Med Sci. 2014; 6(12): 638–642.
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Research Article
Authors

Mustafa Nisari 0000-0001-7469-8921

Publication Date December 30, 2019
Submission Date May 20, 2019
Published in Issue Year 2019

Cite

APA Nisari, M. (2019). Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması. Sağlık Bilimleri Dergisi, 28(3), 172-176. https://doi.org/10.34108/eujhs.567907
AMA Nisari M. Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması. JHS. December 2019;28(3):172-176. doi:10.34108/eujhs.567907
Chicago Nisari, Mustafa. “Civan Percemi (Archilia millefolium)’nin Diyabetik sıçanların karaciğer Dokusundaki Antioksidan Enzimler üzerine Koruyucu Etkisinin araştırılması”. Sağlık Bilimleri Dergisi 28, no. 3 (December 2019): 172-76. https://doi.org/10.34108/eujhs.567907.
EndNote Nisari M (December 1, 2019) Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması. Sağlık Bilimleri Dergisi 28 3 172–176.
IEEE M. Nisari, “Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması”, JHS, vol. 28, no. 3, pp. 172–176, 2019, doi: 10.34108/eujhs.567907.
ISNAD Nisari, Mustafa. “Civan Percemi (Archilia millefolium)’nin Diyabetik sıçanların karaciğer Dokusundaki Antioksidan Enzimler üzerine Koruyucu Etkisinin araştırılması”. Sağlık Bilimleri Dergisi 28/3 (December 2019), 172-176. https://doi.org/10.34108/eujhs.567907.
JAMA Nisari M. Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması. JHS. 2019;28:172–176.
MLA Nisari, Mustafa. “Civan Percemi (Archilia millefolium)’nin Diyabetik sıçanların karaciğer Dokusundaki Antioksidan Enzimler üzerine Koruyucu Etkisinin araştırılması”. Sağlık Bilimleri Dergisi, vol. 28, no. 3, 2019, pp. 172-6, doi:10.34108/eujhs.567907.
Vancouver Nisari M. Civan percemi (Archilia millefolium)’nin diyabetik sıçanların karaciğer dokusundaki antioksidan enzimler üzerine koruyucu etkisinin araştırılması. JHS. 2019;28(3):172-6.