THE DETERMINATION OF SPERM MORPHOLOGY AND DNA DAMAGE IN THE DIFFERENT PATIENT GROUPS

Abstract

In this study, we aimed to investigate the DNA damages and the variations among sperm quality levels in semen samples from healthy individuals and oligozoospermic, insulated asthnenozoospermic, insulated teratozoospermic and oligoasthenoteratozoospermic patients with the factors effecting spermatozoon quality. The sperm samples from healthy individuals and patients diagnosed as insulated oligozoospermia, insulated asthenozoospermia, insulated teratozoospermia, and oligoastheoteratozoospermia in separated groups were used. The number of sperm samples was detected by using Makler camera and their morphologies were evaluated by coloring with Spermac Coloration Assay. To determine the DNA damages among groups, Alkaline Comet Assay method was carried out. According to morphologic results of our study, while the sperm morphology belonging to normozoospermia group was within the normal ranges, the normal sperm morphology in insulated asthenozospermia and insulated oligozoospermia groups was %4 and no significant sperm morphology anomalies were found. In insulated teratozoospermia and oligoasthenoteratozoospermia groups, normal sperm morphology percentages were below %4 and the anomaly percentages were higher than those in the other groups. The results we obtained by using Comet Assay were consistent with morphologic evaluations. As a result, we believe that the Comet Assay can also be guide for determining DNA damages in addition to routine semen analysis and morphologic evaluations.

Keywords

• Spermatozoon
• DNA damage
• comet assay

FARKLI HASTA GRUPLARINDA SPERM MORFOLOJİSİ VE DNA HASARI ARASINDAKİ İLİŞKİNİN BELİRLENMESİ

Abstract

Bu çalışmada, izole oligozoospermi, izole astenozoospermi, izole teratozoospermi ve oligoastenoteratozoospermi hastalarından ve sağlıklı bireylerden alınan semen örneklerinde sperm kalite düzeyleri arasındaki farklılıkların ve DNA hasarının incelenmesi amaçlanmıştır. Çalışmada, izole oligozoospermi, izole astenozoospermi, izole teratozoospermi, oligoastenoteratozoospermi tanısı alan hastalar ile sağlıklı erkek bireylerden alınan sperm örnekleri kullanılmıştır. Sperm örneklerinin sayı ve motilitesi Makler kamera ile belirlendi ve Spermac boyama yöntemi ile boyanarak morfolojileri değerlendirildi. Gruplar arasında sperm hücresi DNA hasarını belirlemek amacıyla alkali comet assay yöntemi kullanıldı. Çalışmamızın morfolojik bulgularına göre; normozoospermi grubuna ait sperm morfolojisi normal sınırlar içinde iken, izole astenozoospermi ve izole oligozoospermi gruplarında normal sperm morfolojisi %4 idi ve kayda değer sperm morfoloji anomalilerine rastlanmadı. İzole teratozoospermi ve oligoastenoteratozoospermi gruplarında ise, normal sperm morfoloji yüzdeleri %4’ ün altında olup, anomali yüzdesi diğer gruplara göre daha fazlaydı. Comet ile incelediğimiz sperm DNA hasarı bulguları morfolojik değerlendirmeler ile uyumluydu. Sonuç olarak, rutin semen analizi ve morfolojik değerlendirmelerin yanı sıra DNA hasarını incelemekte comet assay’in de yol gösterici nitelikte kullanılabileceği kanaatindeyiz.

Keywords

• Spermatozoon
• DNA hasarı
• comet assay

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