HİPOKSİ İLE OLUŞTURULAN MYOKARDİYAL HASAR ÜZERİNE MELATONİNİN ETKİSİ

Year 2004, Volume: 13 Issue: 1, 64 - 72, 01.03.2004

Figen Narin Fatmagül Başarslan Hülya Akgün Aynur Akın Ali Baykan Recep Saraymen Sibel Kuzugüden Selda Yavaşcan

Abstract

Sunulan çalışmada hipoksik hasarın etyopatogenezinde yer alan serbest radikallerin, lipid peroksidasyon ürünlerinin ve antioksidan enzimlerin rolünü değerlendirmek amaçlanmıştır. Çalışmada deney hayvanı olarak tavşan kullanıldı. Tavşanlar; Grup I: Hipoksi grubu (n=9), Grup II: Hipoksi+Melatonin grubu (n=9), Grup III: Kontrol grubu (n=7) olmak üzere üç gruba ayrıldılar. Hipoksi oluşturulmadan önce bazal serum troponin I, kreatin kinaz–MB (CKMB), laktat dehidrojenaz (LDH), plazma glutatyon peroksidaz (GSH-Px), süperoksit dismutaz (SOD), malondialdehit (MDA) ve nitrik oksit (NO) düzeyleri için kan alındı. Grup I, II’ deki deney hayvanları bir fanus içerisinde 5 lt/10 dk akımla %10 oksijen-azot karışımına maruz bırakılarak hipoksi oluşturuldu. İkinci grupta hipoksi oluşturulduktan sonra 5 gün süreyle melatonin (10 mg/kg/gün intraperitoneal) verildi. Tedavi sonunda serum CKMB, LDH ve troponin I, plazma NO, MDA, SOD ve GSH-Px düzeyleri çalışıldı. Tavşanlar sakrifiye edilip myokardiyal NO, MDA, SOD, GSH-Px düzeyleri çalışıldı ve histopatolojik olarak myokard dokusu Hemotoksilen-Eozin boyama ile değerlendirildi. Hipoksi oluşturulan birinci grup, tavşanlarda serum CKMB, LDH, troponin I düzeylerinde artış, plazma SOD’de, GSH-Px’da azalma ve histopatolojik olarak miyokard liflerinde şişme, intertisiyel ödem, disorganizasyon ve nekrozdan oluşan ağır kardiyomyopati tesbit edildi. Hipoksi+ Melatonin uygulanan grupta; plazma SOD düzeylerinde anlamlı artış, doku NO ve MDA düzeylerinde anlamlı düşme tesbit edildi. Histopatolojik incelemede, melatonin grubunda; miyokard liflerinde şişme, intertisiyel ödem ve disorganizasyondan oluşan hafif-orta kardiyomyopati tesbit edildi. Sonuç olarak; melatoninin antioksidan etkinlik sağlayarak hipoksiye bağlı kardiyak hasarı önleyebileceği kanısına varıldı

Keywords

Hipoksi, myokardiyal hasar, malondialdehit, melatonin

Effects of Melatonin on the Myocardial Damage, Induced by Hypoxia

Abstract

The aim of this study was to evaluate the role of antioxidant enyzmes, lipid peroxidation products and free radicals which involve in the etiopathogenesis of the hypoxic damage. The experimental animals were assigned to 5 groups. Group 1: Hypoxia group (n=9), Group II: Hypoxia + melatonin group (n=9), Group III: Control group (n=7). Blood was taken for analysing the levels of basal serum troponin I, creatine kinase-MB (CKMB), lactate dehydrogenase(LDH), plasma glutation peroxidase (GSH-Px), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) before inducing the hypoxia. In the experimental animals of groups I, II, the hypoxia was induced by leaving the animals to breathe in belljar, containing 10 per cent oxygen-nitrogen mixture, for 10 minutes at 5 liter velocity. After inducing the hypoxia melatonin (10 mg/kg/daily & intraperitoneal) was administered to the animals of second group during 5 days. The levels of serum CKMB, LDH and troponin I, plasma NO, MDA, SOD and GSHPx were analysed at the end of the treatment. After sacrificing the experimental animals, tle levels of myocardial NO, MDA, SOD, GSH-Px were analysed and myocardial tissue was examined histopathologically with haematoxylin - eosin stain. The levels of serum-CMKB, LDH and troponin I were found increased; whereas the levels of plasma SOD and GSH-Px were found decreased in the hypoxia-induced group and a heavy cardiomyopathy associated with swelling in myocardial flaments, interstitial edema, disorganization and necrosis was determined. There was a meaningful increase in the levels of plasma SOD but a meaningful decrease in the levels of tissue NO and MDA and a mild and intermediate cardiomyopathy associated with swelling, interstitial edema and disorganization was shown histopathologically in the second group (hypoxia and melatonin). It is shown that the melatonin can protect the hypoxic cardiac damage by being activated as an antioxidant

Keywords

Hypoxia, myocardial damage, malondialdehyde, melatonin

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