MESANE KANSERLİ DOKU KÜLTÜRLERİNDEKİ MİKRONÜKLEUS ÜZERİNE PROPOLİS VE MİTOMİSİN-C’NİN ETKİLERİ

Year 2004, Volume: 13 Issue: 2, 15 - 21, 01.09.2004

Halil Erhan Eroğlu Atilla Tatlışen Yusuf Özkul

Abstract

Bu çalışmada yüzeyel mesane kanserli hastaların transüretral rezeksiyon (TUR) doku örneklerinden hazırlanan tranzisyonel karsinom hücre kültürlerinde mitomisin-C (MMC) ve propolisin mutagenik etkilerine bakıldı. Çalışmamızda mesane kanserli doku kültürlerine 0.1 ml propolis (8 g propolis + %8’lik 15 ml L-Lizin), 1.6 µl mitomisin-C (0.1 mg/ml) ve 0.1 ml L-Lizin (80 mg L-Lizin + 1 ml distile su) ilave edilerek, mikronükleus üzerine etkileri değerlendirildi. Sonuçlarımız propolis ve mitomisinC’nin kontrol grubuna hücrelerdeki mikronükleus (MN) oranını arttırdığını göstermektedir (p<0.05). Hem propolis hem de mitomisin-C mikronükleus oranını arttırıcı etkilerinden dolayı mutagen maddelerdir

Keywords

Mesane kanseri, mikronükleus, mitomisin-C, propolis

Effects of Propolis and Mitomycın-C on Micronucleusin Tissue Cultures of Bladder Cancer

Abstract

In this study, we have tested the mutagenic effects of mitomycin-C (MMC) and propolis on transitional carcinoma cell cultures. Tissue samples were obtained from transurethral resection (TUR) tissue of patients with superficial bladder cancer. In this study, 0.1 ml propolis (8 g propolis + 8% 15 ml L-Lysin), 1.6 µl mitomycin-C (0.1 mg/ml) and 0.1 ml L-Lysin (80 mg L-Lysin + 1 ml distilled water) were added to cancer tissue cultures and the effects of these solutions on the micronucleus (MN) rate were evaluated. Propolis and mitomycin-C increased the micronucleus rate of the cell when compared to untreated control (p<0.05). Our results showed that both propolis and mitomycin-C are mutagenic materials, because of their micronucleus increasing effects

Keywords

Bladder cancer, micronucleus, mitomycin-C, propolis

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