SIÇANLARDA GRELIN UYGULAMASININ BAZI HEMATOLOJIK PARAMETRELER ÜZERINE ETKISİ

Year 2010, Volume: 19 Issue: 3, 202 - 208, 01.12.2010

Naciye Narin Ebru Çetin

Abstract

Bu çalışma sıçanlarda grelin uygulamasının bazı hematolojik parametreler üzerine etkisini incelemek amacıyla yapıldı. Bu amaçla 40 erkek Sprague Dawley ırkı sıçan eşit şekilde 2 gruba ayrılarak kontrol grubuna deri altı yolla 5 gün süreyle %0.9 NaCl, deney grubuna ise deri altı yolla 5 gün süreyle günde 10 nmol/kg dozunda grelin uygulandı. gruplardan kan örnekleri alınarak total lökosit ve eritrosit hematokrit değer, ortalama eritrosit hacmi, ortalama eritrosit hemoglobin konsantrasyonları ile lökosit alt tipleri belirlendi. Deney grubunda, grelin uygulamasına bağlı olarak lenfosit oranında anlamlı bir artma (p<0.05) gözlenirken nötrofil oranında ise önemli bir azalma (p<0.05) tespit edildi. Diğer yandan kontrol ve deney grubundaki hayvanların total eritrosit ve lökosit sayısı ile hemoglobin konsantrasyonu, hematokrit değer, ortalama eritrosit hacmi, ortalama eritrosit hemoglobini, ortalama eritrosit hemoglobin konsantrasyonu ve diğer lökosit alt tiplerinin (monosit, eozinofil ve bazofil) grelin uygulamasından etkilenmediği belirlendi. Sonuç olarak, sıçanlara uygulanan grelinin lenfopoezisi uyarmak suretiyle lenfosit oranını artırabileceği belirlendi

Keywords

Sıçan, grelin, hematolojik parametre

Effect of Ghrelin Administration on Some Hematological Parameters in Rats

Abstract

This study was carried out to determine the influence of ghrelin administration on some haematologic parameters in rats. Forty male Sprague Dawley rats were equally divided into two groups, control group (1 ml physiological saline s.c., for 5 days) and experimental group (ghrelin, s.c., 10 nmol/kg/day, for 5 days). From each animal, blood samples were taken 24 h after the end of treatment, and some hematological parameters were evaluated: total erythrocyte and leukocyte count, hemoglobin concentration, hematocrit value, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration and mean corpuscular valume, and leukocyte differential counts. Lymphocyte percentage was increased (p<0.05), while neutrophil percentage was decreased (p<0.05) due to ghrelin administration. On the other hand, total erythrocyte and leukocyte count, hemoglobin concentration, hematocrit value, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, mean corpuscular valume, and other differential leucocyte counts (monocytes, eosinophils, and basophils) were not affected by ghrelin administration. As a result, it was determined that ghrelin administration to rats may increase lymphocyte ratio by stimulating lymphopoesis

Keywords

Rat, ghrelin, hematologic parameter

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