GIARDIOSIS’Lİ KÖPEKLERDE HEMATOLOJİK VE BİYOKİMYASAL GÖSTERGELERİN DEĞERLENDİRİLMESİ

Year 2011, Volume: 20 Issue: 2, 129 - 136, 01.06.2011

Mustafa Sarı Ali Cesur Onmaz

Abstract

Bu çalışmada, doğal enfekte giardiosis’li köpeklerde trombosit sayısı, protrombin zamanı (PT), aktive edilmiş kısmi tromboplastin zamanı (aPTT), trombin zamanı (TT) ve fibrinojen gibi bazı kan pıhtılaşma parametrelerinin belirlenmesi amaçlanmıştır. Bu parametlerin yanında hematolojik ve biyokimyasal parametreler de ölçülmüştür. Bu amaçla Kayseri yöresindeki iki farklı köpek yetiştirme barınağından toplanan yaşları 1-3 arasında değişen farklı cinsiyette (84’ü erkek, 86’sı dişi) toplam 170 hayvan çalışmaya alınmıştır. Klinik muayeneler sonucunda birkaç günlük yumuşak sulu dışkı şikayeti olan ve pratik antijenik test kitleri ile yapılan testler sonucu Giardia pozitif tespit edilen 60 adet köpek çalışma grubunu oluşturdu (Grup 1). Çalışmanın kontrol grubunu ise yapılan klinik muayenelerde sağlıklı olduğu belirlenen ve antijenik kitlerle Giardia negatif tespit edilen 30 adet köpek oluşturdu (Grup 2). Her iki gruptaki köpeklerden EDTA’lı, Sodyum sitrat’lı ve antikoagulantsız tüplere kan örnekleri alındı. Yapılan hematolojik muayenelerde, Grup 1’de ortalama lökosit değerlerinin kontrol grubu (Grup 2) değerlerinden istatistiksel açıdan daha yüksek (p<0.05) olduğu, ancak biyokimyasal parametreler ile pıhtılaşma faktörlerinin değişmediği belirlendi. Kliniğe getirilen ve şiddetli giardiosis belirlenen iki adet köpekte WBC sayısında artış ve anemi ile birlikte hematokrit ve hemoglobin seviyesinin düştüğü belirlendi. Fibrinojen seviyesinde artış ve TT zamanında uzama gözlendi. Ayrıca kliniğe getirilen bu köpeklerde ortalama ALP, LDH, Üre, Ca ve P değerlerinin normal referans değerlerinden ve Grup 1 ve 2’nin ortalama değerlerinden yüksek, glukoz ve albumin değerlerinin ise düşük olduğu tespit edildi. Sonuçta şiddetli giardiosisli köpeklerin klinik değerlendirilmesinde bu parametrelerin göz önüne alınabileceğine ve hasta sayılarının daha yüksek olduğu ileri çalışmalara ihtiyaç duyulduğu kanısına varılmıştır

Keywords

Giardiosis, protrombin zamanı, aktive parsiyel tromboplastin zamanı, trombin zamanı, köpek

The Evalution of Hematologic and Biochemical Parameters in Dog with Giardiosis

Abstract

The aim of this study was to determine the blood
clotting parameters such as prothrombin time (PT), activated
partial thromboplastin time (aPTT), trombine time (TT) and
fibrinogen in dogs with naturally infected giardiosis.
Additionally, the heamatological and biochemical
parameters were analysed in this study. For this purpose, a
total of 170 animals obtained from two dog shelters in
Kayseri province, aged between 1-3 years, different sex (84
males and 86 females) were included in this study. Sixty
Giardia-positive dogs detected with antigenic practical kits,
suffering from mild watery stools mild watery stools, were
served as Group 1. Thirthy clinically healthy Giardianegative dogs were used as control group (Group 2). Blood
samples were taken in to tubes with EDTA, Sodium-citrate
and tubes without any anticoagulants from the dogs in both
groups. In hematological examinations, the mean total
leukocyte values (WBC) in Group 1 were significantly(p
<0.05) higher than those of control group (Group II),but
none of the biochemical and blood clotting parameters
analyzed presented significant differences. Anemia,
leucocytosis, low haemotocrit and haemoglobin levels were
observed in two dogs showed severe symtomps of Giardiosis
in our clinic. High Fibrinogen levels and prolonged TT were
determined in these dogs. The mean Alkalen Phosphatase
(ALP), Lactat Dehidrogenase (LDH), Urea (BUN), calcium
(Ca) and Phosphorus (P) levels of these two dogs were
higher than those of the both groups and the reference
values, but the mean glucose and albumin levels were lower
than those of the both groups. In conlusion, parameters of
WBC, TT, fibrinogen, ALP, LDH, BUN, Ca, P, glucose,
albumin should be taken into consideration at the clinical
and laboratory evaluation of the dogs with severe clinical
symptoms. Hovewer, further studies including high number
of dogs with severe Giardia infection are needed.

Keywords

Giardiosis, prothrombine time, active partial thromboplastine time, thrombine time, dog

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