KAYSERİ’DEKİ FARKLI BİNALARIN SU DEPOLARINDA LEGIONELLA ARAŞTIRILMASI

Year 2011, Volume: 20 Issue: 1, 9 - 17, 01.03.2011

Zahide Akkaya Yusuf Özbal

Abstract

Dünyanın her yerinde, doğal sularda, hidrotermal alanlarda ve yeraltı su sediementlerinde yaygın olarak bulunan Legionella türleri pleomorfik gram negatif organizmalardır. Hastaneler genellikle geniş ve karışık su sistemlerine sahip ve duyarlı kişilerin yaşadığı ortamlardır. %X oDOışPDGD KDVWDQH RWHO RNXO YH PHVNHQ VX VLVWHPOHULQGH Legionella sp. araştırıldı. Kayseri İl’inde yer alan 8 hastane, 10 okul, 5 otel ve 6 meskenden 01 Eylül-31 Aralık 2008 tarihleri arasında toplam 120 örnek alındı ve mikrobiyolojik yöntemlerle Legionella yönünden araştırıldı. Alınan her bir örnek yoğunlaştırıldıktan sonra sedimentten BCYE-α besiyerine direk ekim yapıldı. Kültürler 37oC’de 10-15 gün inkübasyona bırakıldı ve üçüncü günden itibaren her gün kontrol edildi. Genelde inkübasyondan 3-5 gün sonra gelişen Legionella şüpheli kolonilerden alınarak gram boyama yapıldı. Zayıf gram negatif, çomak şeklinde bakterilerin görülmesi durumunda TSA ve BCYE-α besiyerlerine pasaj edildi. 37oC’de 24-48 saat inkübasyon sonucunda, TSA besiyerinde üreme olmayan ancak BCYE-α besiyerinde üreyen koloniler alınarak lateks aglutinasyon testi ile serotiplendirildi. Toplam 120 örneğin 8’inde Legionella şüpheli bakterilerin ürediği ve bunların gram boyamasında Legionella morfolojilerine benzer gram negatif bakteriler görüldü. Legionella türü olarak belirlenen 8 kültür, lateks aglutinasyon testi ile serotiplendirildi. 16 depo suyundan 3’ünün L.pneumophila serogrup 1, 64 musluk-duş başlığı suyundan üretilen 2 Legionella türünün 1’i L.pneumophila serogrup 1 ve 40 musluk-duş başlığı sürüntüsünden üretilen 3 Legionella türünün 2’si L. pneumophila serogrup 1 olduğu belirlendi. Elde edilen bulgularımız diğer araştırıcıların sonuçları ile uyumlu idi. Epidemiyolojik önemi olan fenotipleri belirlemek için daha geniş çevrede ve moleküler biyolojik metodlar uygulanarak araştırılmalıdır. Turistik konaklama yerleri ve hastanelerin su depoları daha kapsamlı araştırılmalı, gerekli dezenfeksiyon işlemleri yapılmalıdır

Keywords

Legionella, Legionella pneumophila, Lejyoner hastalığı, Kayseri

Legionella Researging in Water Depots’ of Different Buildings in Kayseri

Abstract

Legionella species are pleomorphic gram negative organisms, which are ubiquitous of worldwide in natural freshwater habitats, water in hydrotermal areas and subterrestrial graundwater sediments. Hospitals often have large and complex water distribution systems and a populations of susceptible patients. In this study, Legionellae was researched at hospitals, hotels, schools and dwelling houses water systems. Totaly 120 samples were collected from 8 hospitals, 5 hotels and 6 dwelling houses located in Kayseri metropoliton in between 01 September to 31 December 2008 and it was researched with microbiological methods for Legionella. This study was performed in medical Microbiology laboratory of Medical Faculty of Erciyes University. Each sample was condensed, than cultivated directly from sediment to BCYE-α agar media which most widely used for Legionellae isolation. The cultures were incubated for 10-15 days at 370C and it was checked every day after 3thday of incubation. Colonies usually first appeared after 3-5 days of incubaiton and stained suspect Legionellae colonies by gram staining methods. Gram negative bacilli which possess Legionella species, subcultivated on TSA and on BCYE-α media. After extended cultivation for 20-48 hours incubation period, growth were seen on BCYE-α and not seen on TSA culture. They were serotyped with latexes agglutination’s test.Legionellae suspected colonies grown in 8 of 120 samples were stained with gram staining and appeared gram negative bacilli in shape alike Legionella species morphology. These 8 cultures which were determined Legionella species, serotyped with latex aglutinasyon as L.pneumophila serogrup 1 in 3 of 16 depot’s water, L.pneumophila serogrup 1 in one of 2 Legionella species of 64 tap-douches’ water, and L. pneumophila serogrup 1 in 2 of 3 Legionella species of 40 tap-douches’ track. In this study, Legionellae were isolated and were serogrouped in our area’s institution such as hospital, hotel’s water depots. Obtained finding was been compatible with other searched out results. Extensive environmental studies indicate that molecular biological methods should be use for determining of phenotyping for epidemiology significances. Touristic accommodation places and hospitals must be researched more comprehensive, necessary disinfection treating should be done

Keywords

Legionella, Legionella pneumophila, Legionnaire's disease, Kayseri

References

• 1. Fraser DW, Tsai TR et al. Legionnaires’ disease: description of an epidemic of pneuPRQLD1(QJO-0HG-1197.
• 2. Brenner DJ, Steigerwalt AG and McDade JE. Classification of the Legionnaires’ disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann Intern 0HG-658.
• 3. Barbaree JM, Fields BS. et al. Isolation of protozoa from water associated with a legionellosis outbreak and demonstration of intracellular multiplication of Legionella pneumophila. Appl Environ Microbiol -424.
• 4. Breiman RF, Fields BS. et al. Association of shower use with Legionnaires’ disease. Possible role of amoebae. J. Am Med Assoc -2926.
• 5. Winn WC Jr. Legionella. In: Manual of Clinical Microbiology, Murray, Baron, Pfaller, Tenover and Yolken ( eds) ASM press, Washington DC 1995, pp 533-44.
• 6. Harrison TG and Taylor AG. Phenotypic characteristics of Legionellae. In: Harrison TG, and Taylor AG (eds), A laboratory manual for Legionella. Chichester: Jhon Wiley 6RQVSS-46.
• 7. Edelstein PH. Comprative study of selective media for of Legionellla pneumophila from SRWDEOHZDWHU-&OLQ0LFURELRO 697-699.
• 8. McDade JE, Shepard CC et al. Legionnaires’ disease: isolation of a bacterium and demonstration of its role in other respiraWRU\GLVHDVH1(QJO-0HG -1203.
• 9. Yu VL. Could aspiration be the major mode of transmission for Legionella? Amer J Med -22.
• 10. Blatt SP, Parkinson MD, Pacc E. et al. Nosocomial Legionnaires’ disease: aspiration as a primary mode of transmission. Am J 0FG-22.
• 11. Fry NK, Bangsborg JM, Bernander S, et al.Assesment of intercentre reproducibility and epidemiological concordance Legionella pneumophila serogrup genotyping by amplified fragment length polymorphism analysis. Eur J Clin Microbiol Infect Dis -80