CANDİDA TÜRLERİNİN TANIMLANMASINDA KROMOJENİK BESİYERİNİN DEĞERLENDİRİLMESİ

Year 2013, Volume: 22 Issue: 1, 24 - 30, 01.03.2013

Aslıhan Gültekin Ayşe Nedret Koç Mustafa Altay Atalay

Abstract

Mayalar morfolojik, biyokimyasal ve fizyolojik benzerliklerinden dolayı sıklıkla karıştırılabilir. Bu çalışmanın amacı, sistemik kandidoz şüphesi olan hastalardan izole edilen Candida suşlarının örneklerinin tanımlanmasının değerlendirilmesidir. Kasım 2007 Aralık 2008 ayları arasında sistemik kandidoz hastalarından toplanan 48 farklı örnek (bronkoalveolar lavaj, beyin omurilik sıvısı, steril vücut sıvısı, doku ve kan) çalışmaya dahil edildi. Morfolojik ve biyokimyasal testlerle Candida cinsi olarak tanımlanan mayaların kromojenik besiyeri (CHROMagar ID Candida, Biomerieux, Fransa) ekimi yapıldı. İzole edilen toplam 48 Candida suşları, morfolojik ve biyokimyasal yöntemler kullanılarak 22’si C. albicans, 5’i C. keyfr, 6’sı C. tropicalis, 7’isi C. glabrata, 6’sı C. parapisilosis, biri C. krusei ve biri de C. rugosa olarak tanımlandı. Kromojenik besiyerinde 22 C.albicans, 13 C. tropicalis/C. keyfr ve 15’i diğer Candida türleri olarak tanımlandı. İki C. parapisilosis suşu hatalı olarak tanımlandı. Sonuç olarak, kromojenik besiyerinin, konvansiyonel yöntemlerle birlikte Candida türlerinin erken tanısında faydalı bir yöntem olduğu kanaatine varıldı

Keywords

Candida türleri, kromojenik besiyeri, tanımlama

The Evaluation of Chromogenic Medium in Identification of Candida Species

Abstract

Yeasts can often be confused owing to similarities of their morphological, biochemical and physiological properties. The aim of the study was to evaluate the identification of Candida samples by chromogenic medium which are isolated from systemic candidiasis suspected patients. Between 2007 November and 2008 December, 48 different samples (bronchoalveolar lavage, cerebrospinal fluid, sterile body fluids, tissue, blood) recovered from systemic candidiasis suspected patients have been included in this study. Isolated yeasts identified as Candida with morphological and biochemical tests were inoculated on to chromogenic medium (CHROMagar ID Candida, Biomeriux, France). A total number of 48 Candida isolates were identified as 22 C. albicans, 5 C. keyfr, 1 C. rugosa, 6 C. tropicalis, 7 C. glabrata, 6 C. parapisilosis, 1 C. krusei with morphological and biochemical methods. In chromogenic medium, results were 22 C. albicans, 13 C. tropicalis/C.keyfr and 15 other Candida species. Two C. parapsilosis isolates were identified as incorrect. In conclusion; it is suggested that chromogenic medium with conventional methods is a useful method in early diagnosis of Candida species

Keywords

Candida species, chromogenic medium, identification

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