Abstract
Bu çalışmada streptozotosin (STZ) ile oluşturulan diyabetin sıçan femurundaki kemik yapısı ile mineral yoğunluğunda oluşturduğu değişiklikler ve bu değişiklikler üzerine karnozinin yararlı etkisinin olup olmadığını
belirlemek amaçlanmıştır.
Çalışmada 32 adet 350-400 gr ağırlığında Wistar türü
erkek sıçan kullanıldı. Kontrol, karnozin, diyabet ve
diyabet+karnozin grubu olmak üzere dört farklı grup
oluşturuldu. Deneyden üç hafta sonra sıçanların sağ
femurları çıkarıldı. Uzun eksenine paralel kesilen kemik
dokusu taramalı elektron mikroskobu için hazırlandı ve
ve back scattered electron (BSE) modunda görüntülendi. Ayrıca, energy-dispersive system (EDS) ile mineral
analizleri yapıldı.
Taramalı elektron mikroskobu ile yapılan incelemelerde, kontrol, diyabetik, karnozin ile diyabetik-karnozin
gruplarına ait kemik yapısı ve trabekül kalınlıkları arasında belirgin bir farklılık yoktu. Hem diyafiz hem de
epifiz bölgesi kemik mineral dansitesi (KMD) açısından
karşılaştırıldığında; gruplar arasında istatistiksel olarak
anlamlı bir farklılık yoktu.
Sonuç olarak, STZ ile oluşturulan diyabetin KMD’de
azalmaya yol açtığı ancak karnozinin bu değişimi etkilemediği görülmüştür.
References
- Boldyrev A, Severin SE. The histidine containing dipeptides carnosine and anserine: distribution, properties and biological significance. Adv Enzyme Regul 1990; 30: 175-194.
- Mathiassen B, Nielsen S, Ditzel J, Rodbro P. Longterm bone loss in insulin dependent diabetes mellitus. J Intern Med 1990; 227:325-327.
- Baret-corner E Holbrok TL. Sex differences in osteoporozis in older adults with non-insulin dependent diabetes mellitus. JAMA 1992; 268: 333-337.
- Leiding-Bruckner G, Ziegler R. Diabetes mellitus a risk for osteoporozis? Exp. Clin. Endocrinol Diabetes 2001; 109:493-514.
- Takizawa M, Kameyama K, Maruyama M, Ishida H. Bone loss in type 2 diabetes mellitus-diabetic osteopenia. Nippon Rinsho 2003; 61:287-291.
- Gulewitsch W, Amiradzibi S. Uber das carnosin, eine neue organische base des fleischextractes. Ber Dtsch Chem Ges 1990; 33:1902-1903.
- Bakardijiev A, Bauer K. Biosynthesis, release and uptake of carnosine in primary cultures. Biochemistry 2000; 6:779-782.
- Boldyrev AA. Problems and perspectives in studying the biological role of carnosine. Biochemistry 2000; 65:751-756.
- Brownson C, Hipkiss AR. Carnosine reacts with a glycated protein. Free Radic Biol Med. 2000; 28: 1564-1570.
- Nagai K, Niijima A, Yamano T, Otani H, Okumra N et al. Possible role of L-carnosine in the regulation of blood glucose through controlling autonomic nerves. Exp Biol Med 2003; 228: 1138-1145.
- Kurealla EG, Maltseva VV, Seslavina LS, Stvolinskii SL. hematopoietic stem cells. Bull Exp Biol Med 1991; 112: 966-968. of carnosine on
- Akpan JO. Reduction in blood and urine glucose levels in streptozotocin and alloxan diabetes by phenazine methosulfate. Acta Diabetol Lat 1989; 26:195-201.
- Sözen T. TEMD Osteoporoz ve Diğer Metabolik Ke- mik Hastalıkları Çalışma Grubu. Osteoporoz. Sık Görülen Metabolik Kemik Hastalıkları Kullanım Klavuzu 2008; ss 9-25.
- Albright F, Reifestein EC. Parathyroid glands and metabolic bone disease. Selected studies. Williams and Wilkins Company. Baltimore 1948; 150: 162.
- Meema EF, Meema S. The relationship of diabetes mellitus and body weight to osteoporozis in elderly females. Can Med Ass J 1967; 96: 132-139.
- Loder RT. The influence of diabetes mellitus on the healing of closed fractures. Clin Orthop Relat Res 1988; 232: 210-216.
- Gulyaeva NV, Dupin AM, Levshina IP. Carnosine prevents activation of free-radical lipid oxidation during stress. Bull Exp Biol Med 1989; 107: 148- 152.
- Dahl TA, Midden WR, Hartman PE. Some pravelent biomolecules against singlet oxygen damage. Photochem Photobiol 1988; 47: 357-362.
- Boldyrev A, Severin SE. The histidine containing dipeptides carnosine and anserine: distribution, properties and biological significance. Adv Enzyme Regul 1990; 30: 175-194.
- Yang ZC, Xia K, Wang L, Jia SJ, Li D et al. Asymmetric dimethylarginine reduced erythrocyte deformability in streotozotocin-induced diabetic rats. Microvasc Res 2007; 73: 131-136.
- Korobov VN, Maurisio RB, Mukalov IO, Stvolinski SL. Carnosine stabilization of the normal erythrocyte membranes and in experimental diabetes. Pathol Physiol Exp Ther 2000; 2: 13-15.
- Quinn PJ, Boldyrev AA, Formazuyk VE. Carnosine: its properties, function and potential therapeutic applications. Mol Aspects Med 1992; 13: 379-444.
- Hipkiss A. Carnosine, a protective anti-aging peptide? Int J Biochem 1998; 30: 863-868.
- Kao CH, Tsou CT, Chen CC, Wang SJ. Bone mineral density in patients with noninsulin dependent diabetes mellitus by dual photon absorptiometry. Nuc Med. Common 1993; 14: 373-377.
- Krakauer JC, McKenna MJ, Buderer NF, Rao DS, Whitehouse FW, Parfitt AM. Bone loss and bone turnover in diabetes. Diabetes 1995; 44: 775-782.
- Tuna S, Çetin G, Gökcan G. Tip II diyabetiklerde ke- mik mineral dansitesinin (KMD) değerlendirilmesi. İstanbul Tıp Dergisi 2004; 4: 23-24.
Abstract
This study aims to determine the effects of
streptozotocin-induced diabetic on mineral density and
bone structure in the femur of rat and whether of carnosine has a beneficial effect on these changes.
In this study, 32 male Wistar rats weighing 350-400 gr
were used. Four different groups were formed including
control, carnosine, diabetes and diabetes+
carnosine. After three weeks from experiment, right
femurs of the rats were removed. The bone tissues cut
parallel to long axis were observed by scanning electron microscope in back scattered electron (BSE) mode.
Additionally, mineral analysis was performed with
energy-dispersive system (EDS).
In observations with scanning electron microscopy, no
significant difference of bone structure and trabecular
thickness was found between control, diabetic, carnosine with diabetic-carnosine groups. When we compared all the groups in terms of bone mineral density
(BMD) neither the diaphysis nor the epiphysis of femur
was statistically significant.
As a result, streptozotocin-induced diabetes caused
decrease in BMD, but this change, was not affected by
carnosine.
References
- Boldyrev A, Severin SE. The histidine containing dipeptides carnosine and anserine: distribution, properties and biological significance. Adv Enzyme Regul 1990; 30: 175-194.
- Mathiassen B, Nielsen S, Ditzel J, Rodbro P. Longterm bone loss in insulin dependent diabetes mellitus. J Intern Med 1990; 227:325-327.
- Baret-corner E Holbrok TL. Sex differences in osteoporozis in older adults with non-insulin dependent diabetes mellitus. JAMA 1992; 268: 333-337.
- Leiding-Bruckner G, Ziegler R. Diabetes mellitus a risk for osteoporozis? Exp. Clin. Endocrinol Diabetes 2001; 109:493-514.
- Takizawa M, Kameyama K, Maruyama M, Ishida H. Bone loss in type 2 diabetes mellitus-diabetic osteopenia. Nippon Rinsho 2003; 61:287-291.
- Gulewitsch W, Amiradzibi S. Uber das carnosin, eine neue organische base des fleischextractes. Ber Dtsch Chem Ges 1990; 33:1902-1903.
- Bakardijiev A, Bauer K. Biosynthesis, release and uptake of carnosine in primary cultures. Biochemistry 2000; 6:779-782.
- Boldyrev AA. Problems and perspectives in studying the biological role of carnosine. Biochemistry 2000; 65:751-756.
- Brownson C, Hipkiss AR. Carnosine reacts with a glycated protein. Free Radic Biol Med. 2000; 28: 1564-1570.
- Nagai K, Niijima A, Yamano T, Otani H, Okumra N et al. Possible role of L-carnosine in the regulation of blood glucose through controlling autonomic nerves. Exp Biol Med 2003; 228: 1138-1145.
- Kurealla EG, Maltseva VV, Seslavina LS, Stvolinskii SL. hematopoietic stem cells. Bull Exp Biol Med 1991; 112: 966-968. of carnosine on
- Akpan JO. Reduction in blood and urine glucose levels in streptozotocin and alloxan diabetes by phenazine methosulfate. Acta Diabetol Lat 1989; 26:195-201.
- Sözen T. TEMD Osteoporoz ve Diğer Metabolik Ke- mik Hastalıkları Çalışma Grubu. Osteoporoz. Sık Görülen Metabolik Kemik Hastalıkları Kullanım Klavuzu 2008; ss 9-25.
- Albright F, Reifestein EC. Parathyroid glands and metabolic bone disease. Selected studies. Williams and Wilkins Company. Baltimore 1948; 150: 162.
- Meema EF, Meema S. The relationship of diabetes mellitus and body weight to osteoporozis in elderly females. Can Med Ass J 1967; 96: 132-139.
- Loder RT. The influence of diabetes mellitus on the healing of closed fractures. Clin Orthop Relat Res 1988; 232: 210-216.
- Gulyaeva NV, Dupin AM, Levshina IP. Carnosine prevents activation of free-radical lipid oxidation during stress. Bull Exp Biol Med 1989; 107: 148- 152.
- Dahl TA, Midden WR, Hartman PE. Some pravelent biomolecules against singlet oxygen damage. Photochem Photobiol 1988; 47: 357-362.
- Boldyrev A, Severin SE. The histidine containing dipeptides carnosine and anserine: distribution, properties and biological significance. Adv Enzyme Regul 1990; 30: 175-194.
- Yang ZC, Xia K, Wang L, Jia SJ, Li D et al. Asymmetric dimethylarginine reduced erythrocyte deformability in streotozotocin-induced diabetic rats. Microvasc Res 2007; 73: 131-136.
- Korobov VN, Maurisio RB, Mukalov IO, Stvolinski SL. Carnosine stabilization of the normal erythrocyte membranes and in experimental diabetes. Pathol Physiol Exp Ther 2000; 2: 13-15.
- Quinn PJ, Boldyrev AA, Formazuyk VE. Carnosine: its properties, function and potential therapeutic applications. Mol Aspects Med 1992; 13: 379-444.
- Hipkiss A. Carnosine, a protective anti-aging peptide? Int J Biochem 1998; 30: 863-868.
- Kao CH, Tsou CT, Chen CC, Wang SJ. Bone mineral density in patients with noninsulin dependent diabetes mellitus by dual photon absorptiometry. Nuc Med. Common 1993; 14: 373-377.
- Krakauer JC, McKenna MJ, Buderer NF, Rao DS, Whitehouse FW, Parfitt AM. Bone loss and bone turnover in diabetes. Diabetes 1995; 44: 775-782.
- Tuna S, Çetin G, Gökcan G. Tip II diyabetiklerde ke- mik mineral dansitesinin (KMD) değerlendirilmesi. İstanbul Tıp Dergisi 2004; 4: 23-24.
Details
| Other ID | JA69GA64KR |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2013 |
| Submission Date | December 1, 2013 |
| Published in Issue | Year 2013 Volume: 22 Issue: 3 |