Clitocybe geotropa TÜRÜ MANTARDAN ELDE EDİLEN EKSTRAKTLARIN MDA-MB-231 HÜCRE HATTI ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİNİN İNCELENMESİ

Year 2017, Volume: 26 Issue: 1, 0 - 0, 01.03.2017

Berrak Altınsoy S.furkan Konca Huriye Aksu Büşra Kurt Hakan Allı Çağrı Şakalar

Abstract

Genel Bilgi: Mantarların tıbbi amaçlı olarak kullanımlarının geçmişi Asya bölgesi için oldukça eskiye dayanmaktadır ve son zamanlarda Batı’da kullanımları Kabul
görmektedir. Makro mantarlardan izole edilen bileşenlerin veya ekstrelerin antimikrobiyal, anti-viral,
antitümör, anti-alerjik, immünomodülatör, anti- enflamatuar, anti-aterojenik, hipoglisemik ve hepatoprotektif etki gibi çeşitli biyolojik aktivitelerinin olduğu
bildirilmiştir.
Amaç: Bu çalışmada, Clitocybe geotropa (Bull.)
Quél.’den elde edilen etanol ve metanol ekstrelerinin
meme kanseri hücreleri üzerine etkinliği araştırılmıştır.
Yöntem: Bu çalışmada mantar ekstrelerinin verimi,
belli ağırlıktaki kuru materyalden elde edilen etanol
ekstresinin (% 28,2) ve metanol ekstresinin (% 30,6)
ağırlıklarına göre g/100g olarak ifade edilerek belirlenmiştir. Ekstrelerin sitotoksik etkileri Trypan Blue analiziyle belirlenmiştir.
Bulgular: Etanol ve metanol ekstrelerinin total fenolik
içeriği sırasıyla 217,15±0,02 ve 138,39±0,01; total flavanoit içeriği 152,28±0,03 ve 111,95±0,02 mgCE/g
olarak ölçülmüştür. C. geotropa için 50–400 µg/mL
konsantrasyon değerlerindeki metanol ekstrelerinin
MDA-MB-231 hücre hattı üzerinde sitotoksik etkisi
gözlenmezken, etanol ekstresi % 51,5; 400 μg/ml, %
17,9; 200 μg/ml, % 6,6; 100 μg/ml uygulanan konsantrasyonlarda anlamlı düzeyde sitotoksik etki göstermiştir.
Sonuç: Clitocybe geotropa MDA-MB-231 hücre hattı
üzerinde etkili antikanser ajan olarak kullanılma potansiyeline sahip bir mantar türüdür.

Keywords

C. geotropa, toplam fenolik, toplam flavanoit, sitotoksite

Cytotoxic Activity of Two Extracts from the Mushroom Clitocybe Geotropa in Mda-Mb-231Cell Line

Abstract

Background: The medical use of mushrooms has a very
long tradition in Asia and is nowadays becoming more
accepted in the West. It is reported that the extracts or
isolated constituents from macrofungi have biological
activities such as antimicrobial, anti-viral, anti-tumour,
anti-allergic, immunomodulating, anti-inflammatory,
antiatherogenic, hypoglycaemic and hepatoprotective
activities.
Objective: In this study, cytotoxic activity of ethanol
extracts (EE) and methanol (ME) of Clitocybe geotropa
on MDA-MB-231 cell line were investigated.
Method: The yield of mushroom extracts was calculated for EE and ME 30.6% and 28.2%, respectively.
Cytotoxic effects of the extracts were determined by
Trypan Blue assay. Results: The total phenolic contents of EE and ME were
217.15±0.02, 138.39±0.01; total flavonoid contents
152.28±0.03, 111.95±0.02 mgCE/g, respectively. The
ME of mushroom’s 50–400 µg/mL concentrations did
not show any cytotoxic effect in MDA-MB-231 cell line
while the EE of C. geotropa % 51,5; 400 μg/ml, % 17,9;
200 μg/ml, % 6,6; 100 μg/ml had cytotoxicity.
Conclusion: The mushroom, Clitocybe geotropa, could
be used as a potential anticancer agent for MDA-MB- 231 cell line.

Keywords

C. geotropa, total phenolic, total flavonoid, cytotoxicity

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