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DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI

Year 2017, Volume: 26 Issue: 2, 126 - 132, 01.07.2017

Abstract

Bu çalısmada, deneysel ketozis oluşturulan koyunlarda
% 20’lik total parenteral lipit emülsiyonları’nın (TPLE)
% 30 Dekstroz tedavisine karşı etkinliği araştırıldı.
Onsekiz adet gebe olmayan ve laktasyon dönemi dışındaki koyunlar her grupta 6 adet koyun olacak şekilde üç
gruba ayrıldı. Bütün gruplarda deneysel ketozis oluşturuldu. Bu amaçla koyunların herbirine üç gün boyunca
derialtı phlorhizin enjekte edildi (100 mg/kg) ve sınırlı
yem (toplam rasyonun ¼’ü) verildi. Tüm gruplarda
ketozise ait klinik belirtiler oluşturulduktan sonra birinci gruba kontrol amacıyla % 0,9 NaCl, ikinci gruba standart tedavi amacıyla % 30 Dekstroz ve üçüncü gruba %
20'lik TPLE damar içi yola uygulandı. Dekstroz uygulaması sonrasında NEFA düzeyleri önemli oranda azaldı.
Lipit emülsiyonu verilenlerde ise NEFA düzeylerindeki
artış istatistikî açıdan önemli bulunmadı (p=0.05). Bu
grupta trigliserid ve glukoz düzeyleri TPLE uygulamasını takiben diğer gruplara oranla yüksek bulundu. Koyunlarda ketozise ait klinik belirtiler yalnız lipit emülsiyonu verildikten sonra belirgin olarak düzeldi. Sonuç
olarak deneysel açlık ketozisi oluşturulan koyunlarda %
30’luk Dekstrozun NEFA düzeylerini azaltmasına rağmen, % 20 TPLE’nin glikoz ve trigliserid düzeylerinde
artış sağlayıp dekstroz uygulamalarına göre enerji açığını kapattığı belirlendi. Bu nedenle TPLE’nın, açlık
ketozisinin klinik belirtilerinin düzeltilmesinde etkili
olabileceği değerlendirildi.

References

  • 1. Bergman EN. Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Am J Physiol 1973; 215: 865-873.
  • 2. Everts H, Kuiper H. Energy intake and pregnancy toxemia in profilic ewes. Fifth International Conference on Production Diseases in Farm Animals, Uppsala-Sweden 1983; pp 133-136.
  • 3. Pethick DW, Lindsay DB. Metabolism of ketone bodies in pregnant sheep. Br J Nutr 1982; 48: 549- 563.
  • 4. Baird GD, Heitzman RJ, Hibbitt KG. Effects of starvation on intermediary metabolism in the lactating cow. Biochem J 1972; 128: 1311-1318.
  • 5. Bergman EN. Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Am J Physiol 1973; 215: 865-873.
  • 6. Bickhardt K, Henze P, Sallmann HP. GlucoseStoffwechselsto¨ rungen bei erwachsenen Schafen und ihre Behandlungen. DVG Tagung ‘‘Krankheiten Der Kleinen Wiederka¨uer, Giessen 1993: 92–100.
  • 7. Henze P, Pickhardt K, Fuhrmann H, Salman HP. Spontaneous pregnancy toxemia (ketosis) in sheep and the role of insulin. J Vet Med 1998; A45: 255-266.
  • 8. Laffel L. Ketone Bodies: a Review of physiology, pathophysiology and application of monitoring to diabetes. Diabetes Metab Res Rev 1999; 15: 412-426.
  • 9. Mitchell GA, Kassovska-Bratinova S, Boukaftane Y, et al. Medical aspects of ketone body metabolism. Clin Invest Med 1995; 18: 193-216.
  • 10. Ranaweera A, Ford EJ, Evans J. Gluconeogenesis from glycerol by ketotic sheep pregnant with twins. Res Vet Sci 1981; 30: 303-808.
  • 11. Lyle RR, Deboer G, Mılls SE, et al. Glucose kinetics, plasma metabolites, and endocrine responses during experimental ketosis in steers. J Dairy Sci 1984; 67: 2255-2264.
  • 12. Aslan V, Astı RN, Tiftik AM, Eksen M. Effect of niacin on blood metabolities, Rumen protozoa, insulin levels and fatty liver in experimentally induced ketosis in ewes. SÜ Vet Fak Der 1988;4: 109-121.
  • 13. Başoğlu A, Turgut K, Eksen M, ve ark. Effect of phlorihizin-induced ketozis on riboflavin and niacin levels on sheep. SÜ Vet Fak Derg 1993; 9: 58-63.
  • 14. Waitzberg DL, Torrinhas RS, Jacintho TM. New Parenteral Lipid Emulsions for Clinical Use. J Parenter Enteral Nutr 2006; 30: 351-367.
  • 15. Gültekin F, Alagözlü H. Nutrisyon parenteral beslenme. T Klin Tıp Bilimleri 1993;13: 28-36.
  • 16. Picard J, Ward C, Zumpe R, et al. Guidelines and the adoption of lipid rescue therapy for local anaesthetic toxicity. Anaesthesia 2009; 64: 122– 125.
  • 17. Kayıpmaz AE, Gülalp B, Benli S. Lipofilik ajan toksisitesinde yeni ufuklar. JAEM 2011; 80-85.
  • 18. Ozcan MS, Weinberg G. Intravenous lipid emulsion for the treatment of drug toxicity. J Intensive Care Med 2014; 29: 59-70.
  • 19. Muller SH, Diaz JH, Kaye AD. Clinical applications of intravenous lipid emulsion therapy. J Anesth 2015; 29: 920-926.
  • 20. Heitmann RN, Dawes DJ, Sensenig SC. Hepatic ketogenesis and peripheral ketone body utilization in the ruminant. J. Nutr 1987; 117: 1174-1180.
  • 21. Baird GD, Heitzmaa RJ. Mode of action of a glucocorticoid on bovine intermediary metabolism Possible role in controlling hepatic ketogenesis. Biochim Biophys Acta 1971; 252:184 -198.
  • 22. Ranaweera A, Ford EJ, Evans J. Gluconeogenesis from glycerol by ketotic sheep pregnant with twins. Res Vet Sci 1981; 30: 303-808.
  • 23. Marteniuk JV, Herdt TH. Pregnancy toxemia and ketosis of ewes and does. Vet Clin North Am Food Anim Pract 1988; 4: 307-315.
  • 24. Jeffrey M, Higgins RJ. Brain lesions of naturally occurring pregnancy toxemia of sheep. Vet Pathol 1992; 29: 301-307.
  • 25. Ferris TF, Herdson PB, Dunnill MS, Lee MR. Toxemia of pregnancy in sheep: a clinical, physiological, and pathological study. J Clin Invest 1969; 48: 1643-1655.
  • 26. Wastney ME, Wolff JE, Bickerstaffe R. Glucose turnover and hepatocyte glucose production of starved and toxaemic pregnant sheep. Aust J Biol Sci 1983; 36: 271-284.
  • 27. Sigurdsson H. The effects of pregnancy and feding on the insulin and glucose concentration in blood of ewes in late pregnanacy. Acta Vet Scand 1988; 29: 401-405.
  • 28. Harmeyer J, Schlumbohm C. Pregnancy impairs ketone body disposal in late gestating ewes: implications for onset of pregnancy toxaemia. Res Vet Sci 2006; 81: 254-264.

The Effects of Treatment With Total Parenteral Lipid Emultion in Sheep Experimentally Induced Starvation Ketosis

Year 2017, Volume: 26 Issue: 2, 126 - 132, 01.07.2017

Abstract

In this study, the effects of Total Parenteral Lipid
Emulsions (TPLE) and 30% Dekstroz was compared to
treatment of experimentally induced starvation ketosis
in sheep. Eighteen nonpregnant, nonlactating sheep
were randomly divided into 3 different study groups
each containing six sheep. Subcutan phloryzin (100 mg/
kg) was injected together with feed restriction (1/4 of
total ration) to induce the ketosis and lipid mobilisation.
After clinical symptoms for ketosis in all groups, for
control 0,9% NaCl in first group, 30% Dekstroz in
second group IV and 20% TPLE in third group IV were
administered to the each sheep in groups. Mean NEFA
levels was a tendency to decrease after dextrose
administration in group administered dextrose. In TPLE
group, mean NEFA levels were not siginificantly higher
after TPLE administraion (p=0.05). Mean triglyceride
and glucose levels in sheep given TPLE were
significantly higher than those of other groups. Clinical
signs was recovered after in group given TPLE only. In
conclusion, although 30% dextroz treatment was the
most effective in decreasing of NEFA level, 20% TPLE
inhibited clinical signs of starvation ketozis in sheep by
providing urgent energy demand with increase of
trigliserid and glucose levels. For this reason, TPLE was
evaluated to be effective in correcting the clinical
symptoms of fasting ketosis.

References

  • 1. Bergman EN. Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Am J Physiol 1973; 215: 865-873.
  • 2. Everts H, Kuiper H. Energy intake and pregnancy toxemia in profilic ewes. Fifth International Conference on Production Diseases in Farm Animals, Uppsala-Sweden 1983; pp 133-136.
  • 3. Pethick DW, Lindsay DB. Metabolism of ketone bodies in pregnant sheep. Br J Nutr 1982; 48: 549- 563.
  • 4. Baird GD, Heitzman RJ, Hibbitt KG. Effects of starvation on intermediary metabolism in the lactating cow. Biochem J 1972; 128: 1311-1318.
  • 5. Bergman EN. Glucose metabolism in ruminants as related to hypoglycemia and ketosis. Am J Physiol 1973; 215: 865-873.
  • 6. Bickhardt K, Henze P, Sallmann HP. GlucoseStoffwechselsto¨ rungen bei erwachsenen Schafen und ihre Behandlungen. DVG Tagung ‘‘Krankheiten Der Kleinen Wiederka¨uer, Giessen 1993: 92–100.
  • 7. Henze P, Pickhardt K, Fuhrmann H, Salman HP. Spontaneous pregnancy toxemia (ketosis) in sheep and the role of insulin. J Vet Med 1998; A45: 255-266.
  • 8. Laffel L. Ketone Bodies: a Review of physiology, pathophysiology and application of monitoring to diabetes. Diabetes Metab Res Rev 1999; 15: 412-426.
  • 9. Mitchell GA, Kassovska-Bratinova S, Boukaftane Y, et al. Medical aspects of ketone body metabolism. Clin Invest Med 1995; 18: 193-216.
  • 10. Ranaweera A, Ford EJ, Evans J. Gluconeogenesis from glycerol by ketotic sheep pregnant with twins. Res Vet Sci 1981; 30: 303-808.
  • 11. Lyle RR, Deboer G, Mılls SE, et al. Glucose kinetics, plasma metabolites, and endocrine responses during experimental ketosis in steers. J Dairy Sci 1984; 67: 2255-2264.
  • 12. Aslan V, Astı RN, Tiftik AM, Eksen M. Effect of niacin on blood metabolities, Rumen protozoa, insulin levels and fatty liver in experimentally induced ketosis in ewes. SÜ Vet Fak Der 1988;4: 109-121.
  • 13. Başoğlu A, Turgut K, Eksen M, ve ark. Effect of phlorihizin-induced ketozis on riboflavin and niacin levels on sheep. SÜ Vet Fak Derg 1993; 9: 58-63.
  • 14. Waitzberg DL, Torrinhas RS, Jacintho TM. New Parenteral Lipid Emulsions for Clinical Use. J Parenter Enteral Nutr 2006; 30: 351-367.
  • 15. Gültekin F, Alagözlü H. Nutrisyon parenteral beslenme. T Klin Tıp Bilimleri 1993;13: 28-36.
  • 16. Picard J, Ward C, Zumpe R, et al. Guidelines and the adoption of lipid rescue therapy for local anaesthetic toxicity. Anaesthesia 2009; 64: 122– 125.
  • 17. Kayıpmaz AE, Gülalp B, Benli S. Lipofilik ajan toksisitesinde yeni ufuklar. JAEM 2011; 80-85.
  • 18. Ozcan MS, Weinberg G. Intravenous lipid emulsion for the treatment of drug toxicity. J Intensive Care Med 2014; 29: 59-70.
  • 19. Muller SH, Diaz JH, Kaye AD. Clinical applications of intravenous lipid emulsion therapy. J Anesth 2015; 29: 920-926.
  • 20. Heitmann RN, Dawes DJ, Sensenig SC. Hepatic ketogenesis and peripheral ketone body utilization in the ruminant. J. Nutr 1987; 117: 1174-1180.
  • 21. Baird GD, Heitzmaa RJ. Mode of action of a glucocorticoid on bovine intermediary metabolism Possible role in controlling hepatic ketogenesis. Biochim Biophys Acta 1971; 252:184 -198.
  • 22. Ranaweera A, Ford EJ, Evans J. Gluconeogenesis from glycerol by ketotic sheep pregnant with twins. Res Vet Sci 1981; 30: 303-808.
  • 23. Marteniuk JV, Herdt TH. Pregnancy toxemia and ketosis of ewes and does. Vet Clin North Am Food Anim Pract 1988; 4: 307-315.
  • 24. Jeffrey M, Higgins RJ. Brain lesions of naturally occurring pregnancy toxemia of sheep. Vet Pathol 1992; 29: 301-307.
  • 25. Ferris TF, Herdson PB, Dunnill MS, Lee MR. Toxemia of pregnancy in sheep: a clinical, physiological, and pathological study. J Clin Invest 1969; 48: 1643-1655.
  • 26. Wastney ME, Wolff JE, Bickerstaffe R. Glucose turnover and hepatocyte glucose production of starved and toxaemic pregnant sheep. Aust J Biol Sci 1983; 36: 271-284.
  • 27. Sigurdsson H. The effects of pregnancy and feding on the insulin and glucose concentration in blood of ewes in late pregnanacy. Acta Vet Scand 1988; 29: 401-405.
  • 28. Harmeyer J, Schlumbohm C. Pregnancy impairs ketone body disposal in late gestating ewes: implications for onset of pregnancy toxaemia. Res Vet Sci 2006; 81: 254-264.
There are 28 citations in total.

Details

Other ID JA34JV25JY
Journal Section Research Article
Authors

Reyda Kıyıcı Şıklaroglu This is me

Vehbi Güneş This is me

Publication Date July 1, 2017
Submission Date July 1, 2017
Published in Issue Year 2017 Volume: 26 Issue: 2

Cite

APA Şıklaroglu, R. K., & Güneş, V. (2017). DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI. Sağlık Bilimleri Dergisi, 26(2), 126-132.
AMA Şıklaroglu RK, Güneş V. DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI. JHS. July 2017;26(2):126-132.
Chicago Şıklaroglu, Reyda Kıyıcı, and Vehbi Güneş. “DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi 26, no. 2 (July 2017): 126-32.
EndNote Şıklaroglu RK, Güneş V (July 1, 2017) DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI. Sağlık Bilimleri Dergisi 26 2 126–132.
IEEE R. K. Şıklaroglu and V. Güneş, “DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI”, JHS, vol. 26, no. 2, pp. 126–132, 2017.
ISNAD Şıklaroglu, Reyda Kıyıcı - Güneş, Vehbi. “DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi 26/2 (July 2017), 126-132.
JAMA Şıklaroglu RK, Güneş V. DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI. JHS. 2017;26:126–132.
MLA Şıklaroglu, Reyda Kıyıcı and Vehbi Güneş. “DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi, vol. 26, no. 2, 2017, pp. 126-32.
Vancouver Şıklaroglu RK, Güneş V. DENEYSEL KETOZİS OLUŞTURULAN KOYUNLARDA PARENTERAL LİPİT EMÜLSİYONLARININ TEDAVİ ÜZERİNE ETKİLERİNİN ARAŞTIRILMASI. JHS. 2017;26(2):126-32.