SIĞIRLARDA EMBRİYO TRANSFERİNDE CIDR İLE SENKRONİZE EDİLEN DONÖRLERE ÖSTRUS ÖNCESİ GERÇEKLEŞTİRİLEN ÇİFT PGF2α UYGULAMALARININ ELDE EDİLEN EMBRİYOLARIN KALİTESİ VE SAYISI ÜZERİNE ETKİLERİ

Year 2017, Volume: 26 Issue: 3, 204 - 210, 01.12.2017

Uğur Kara Tayfur Bekyürek

Abstract

Bu çalışmada, sığırlarda embriyo transferinde östrus
öncesi uygulanan çift PGF2α uygulamalarının elde edilen
embriyoların kalitesi ve sayısı üzerine etkileri araştırıldı. Araştırmada, 5-7 yaşlı Holştayn ırkı, herhangi bir
sağlık ve reprodüktif problemi bulunmayan, siklusları
düzenli seyreden 20 baş Holştayn ırkı inek iki gruba
ayrıldı (n=10). Donörlere östrus siklusunun herhangi
bir gününde CIDR uygulandı. CIDR uygulandıktan sonra
süperovulasyon amacıyla yedinci günden başlayarak
dört gün süreyle 12 saat aralıklarla azalan dozlarda
(80:80 mg, 60:60mg, 40:30 mg, 30:20 mg) toplam 400
mg FSH kas içi yolla uygulandı. Daha sonra corpus
luteumu (CL) lize etmek amacıyla Grup I’de 5. FSH enjeksiyonuyla birlikte tek doz 500 μg Cloprostenol ve
Grup II’de 5. ve 6. FSH enjeksiyonuyla birlikte çift doz
toplam 1000 μg kas içi enjekte edildi. Her iki grupta da
6. FSH enjeksiyonuyla birlikte CIDR uzaklaştırıldı.
Cloprostenol enjeksiyonundan 24 saat sonra günde üç
kez donörlerin östrusları takip edildi ve östrus başlangıcından itibaren 12 saat ara ile üç kez tohumlandı. Embriyolar tohumlamayı takiben yedinci günün sonunda
uterus yıkaması ile toplandı.
Sonuç olarak, iki protokol arasında embriyo sayısı ve
kalitesi açısından fark bulunamamıştır. Ancak donörlere
östrustan önce çift prostaglandin uygulamasının embriyo sayısı ve kalitesi üzerine sayısal olarak olumlu etkisinin olduğu kanaatine varılmıştır

Keywords

İnek, CIDR, Prostaglandin F2α, Embriyo transferi

The Effect of Double Pgf2α Administrations Applıed to Donor Before Oestrus With Synchronized Cidr on the Number and Qualıty of Obtained Embryos DuringEmbryo Transfer in Cattle

Abstract

In this study, the effect of embryo transfer applications
from pre-applied double-PGF2α before the oestrus
period on the number and quality of the embryos in
cattle were investigated. In the study, 20 Holstein cows
aged 5 to 7 years and do not have any health and
reproductive problems and had regular cycles were
divided into two groups (n = 10). CIDR was applied to
donors in any one day of estrous cycle. İn order to
superovulation decreasing FSH doses were
intramuscularly administered after CIDR application by
starting from 7th day with 12-hour intervals for four
days (mg/ 80:80 mg, 60:60 mg, 40:30 mg, 30:20 mg)
which was totally 400 mg. And later, in order to lyses
corpus luteum with 5th FSH injections with a single
dose 500 μg Cloprostenol and with 5th and 6th FSH
injections with a double dose (500 μg+500 μg)
Cloprostenol intramuscularly injected to Group I and
Group II, respectively. In both Groups, CIDR was
removed at the same time with 6th FSH injections.
Donors’ oestrus were followed up after 24 hours of
cloprostenol injection three times a day and from the
beginning of estrus with a 12-hours intervals three
times inseminated. Embryos were collected at the end
of the 7th day following the insemination, by uterus
washing (flush).
In conclusion, no statistically significant differences
between the two protocols was found in terms of
embryo quality and number, but it was concluded that
double-prostaglandin applications to the donors’ before
the oestrus, had positive impact on the quality and
number of embryos.

Keywords

Cattle, CIDR, Prostaglandin F2α, Embryo transfer

References

• 1. Alaçam E. Evcil Hayvanlarda Reprodüksiyon, Sun`i Tohumlama, Doğum ve İnfertilite (1.Baskı). Konya 1994;pp 1-385.
• 2. Mapletoft RJ. The technology of embryo transfer. IETS, Montreal 1987; pp 2-41.
• 3. Sağırkaya H. Sığırlarda embryo transfer uygulaması ve Türkiye açısından önemi. Uludağ Üniv Vet Fak Derg 2009; 28 :11-19.
• 4. Bó GA, Carballo Guerrero D, Adams GP. Alternative approaches to setting up donor cows for superovulation. Theriogenology 2008; 69:81–87.
• 5. Armstrong DT. Recent advances in superovulation of cattle. Theriogenology 1993; 39:7-24.
• 6. Akyol N. Sığır embriyo transferinde hormon kullanımı. Lalahan Hay Araşt Enst Derg 2001; 4:95- 105.
• 7. Bülbül B, Dursun Ş. İneklerde süperovulasyon cevabına etki eden faktörler. Hay Araş Derg 2005; 15:16-25.
• 8. Seidel GE, Seidel SM. Training manual for embryo transfer in cattle. FAO Animal. Production and health paper 77. Erişim: [http://www.fao.org/ DOCREP/ 004/T0117E/T0117 E00.htm], Erişim Tarihi: 15 Temmuz 2015.
• 9. SPSS. Statistical Package Social Science. SPSS 11.5, SPSS Inc, 1999.
• 10. Yaakub H, O’Callaghan D, Boland MP. Effect of type and quantity of concentrates on superovulation and embryo yield in beef heifers. Theriogenology 1999; 51:1259-1266.
• 11. Köse M, Dursun Ş, Bülbül B ve ark. İsviçre esmeri ineklerde FSH ile süperovulasyon ve embriyo transferi çalışmaları. Hayvancılık Araştırma Dergisi 2006; 16:1-6.
• 12. Nakajima A, Hiraizumi S, Onodera K, et al. The use of bovine anti-PMSG serum in beef cattle after PMSG-superovulation. J Vet Med Sci 1992; 54:95
• 13. Sartori R, Suarez-Fernandez CA, Monson RL, et al. Improvement in recovery of embryos/ova using a shallow uterine horn flushing technique in superovulated Holstein heifers. Theriogenology 2003; 60:1319-1330.
• 14. Selk G. Embryo transfer in cattle. 1996, Erişim: [http://osuextra.okstate.edu/pdfs/F3158web.pdf], Erişim Tarihi: 15 Temmuz 2015
• 15. Gordon IR. Reproductive technologies in farm animals, CAB International, Cambridge 2005.
• 16. Novotny F, Hajurka J, Macak V. Relationship between blood serum progesterone levels in cattle donors and the yield and quality of embryos. Bull Vet Inst Pulawy 2005; 49:49-52
• 17. Dieleman SJ, Bevers MM, Vos PLAM et al. PMSG/ anti-PMSG in cattle: A simple and efficient superovulatory treatmen. Theriogenology 1993; 39:25-42.
• 18. Gonzalez A, Wang H, Carruthers TD, et al. Increased ovulation rates in PMSG-stimulated beef heifers treated with a monoclonal PMSG antibody. Theriogenology 1994; 33:519-529.
• 19. Kim IH, Son DS, Yeon SH, et al. Effect of dominant follicle removal before superstimulation on follicular growth, ovulation and embryo production in Holstein cows. Theriogenology 2001; 55:937-945.
• 20. Sugano M, Shinogi T. Superovulation induction in Japanese Black cattle by a single intramuscular injection of hMG or FSH dissolved in polyvinylpyrrolidone. Anim Reprod Sci 1999; 55:175-181.
• 21. Lopes da Costa L, Chagas e Silva J, Robalo Silva J. Superovulatory response, embryo quality and fertility after treatment with different gonadotrophins in native cattle. Theriogenology 2001; 56:65- 77.
• 22. Hasler JF. Current status and potential of embryo transfer and reproduction in dairy cattle. J Dairy Sci 1992; 75:2857-2879.
• 23. Kanitz W, Becker F, Schneider F et al. Superovulation in cattle: Practical aspects of gonadotropin treatment and insemination. Reprod Nutr Dev 2002; 42:587-599.
• 24. Mapletoft RJ, Steward KB, Adams GP. Superovulation in perspective. Bioniche Animal Health Customer Service 2002.
• 25. Quaresma MA, Lopes da Costa L, Robalo Silva J. Superovulation of Mortelenga cows with two FSH preparations (FSH-P and FOLLTROPIN). Revista Portuguesa de Ciências Veterinárias 2003; 98:81- 84.
• 26. Mapletoft RJ, Steward KB, Adams GP. Recent advances in superovulation in cattle. Reprod Nutr Dev 2002; 42:601-611.
• 27. Adams GP, Matteri RL, Kastelic JP et al. Association between surges of follicle stimulating hormone and the emergence of follicular waves in heifers. J Reprod Fertil 1992; 94:177-188.
• 28. Nogueira MFG, Barros BJP, Teixeira AB, et al. Embryo recovery and pregnancy rates after the delay of ovulation and fixed-time insemination in superstimulated beef cows. Theriogenology 2002; 57:1625-1634.
• 29. Mikkola M, Mäntysaari P, Tammiranta N, et al. Effect of dietary protein on embryo recovery rate and quality in superovulated heifers. Anim Reprod Sci 2005; 87:193-202.
• 30. Callesen H, Greve T, Hyttel P. Preovulatory endocrinology and oocyte maturation in superovulated cattle. Theriogenology 1986; 25:71-86.
• 31. Greve T, Callesen H, Hyttel P, et al. The effects of exogenous gonadotropins on oocyte and embryo quality in cattle. Theriogenology 1995; 43:41-50.
• 32. Vos PLAM, Van de Leemput EE, Zeinstra EC, et al. Postponement of the preovulatory LH surge does not impair the developmental potential of in vivo matured oocytes from eCG/PG-superovulated heifers. Theriogenology 1996; 45: 329 (abstract).