PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ

Year 2017, Volume: 26 Issue: 3, 223 - 226, 01.12.2017

Özgür Er Burhan Can Çanakçı Öznur Tuncay Yasemin Kahraman

Abstract

Pulpa ve periradiküler doku hastalıklarının çoğu hem
aerop hem de anaerop mikroorganizmaları içeren karışık bir mikroflora ile ilişkilidir. Hekim tedavi ile ilgili
etkili bir yöntem belirleyebilmek için mikroorganizma
varlığı ile endodontik hastalık arasındaki yakın ilişkiyi
kavramalıdır.
Endodontik enfeksiyonlardaki bakteri türlerini ortaya
çıkarmak için kültür ve moleküler metodlar kullanılmaktadır. Son yıllarda moleküler yöntemlerdeki gelişmelere rağmen her iki metodun da bir takım dezavantajları bulunmaktadır.
Elektronik burun yöntemi, insan doku duyusunu taklit
edebilen bir elektronik sistemi tanımlar. Elektronik
burun sistemleri tıp alanında ve mikrobiyolojik araştırmalarda başarıyla kullanılmaktadır. Bu sistemlerin en
önemli özelliği, koku çeşitlerini çok kısa zamanda insan
burnundaki hassasiyet derecesinde ayrıştırabilmesi ve
sonucu objektif olarak sunmasıdır.
Bu çalışmada insan diş köklerinden alınan örnekler
elektronik burun sistemine tabi tutulmuş ve
makroskobik ve mikroskobik analizleri yapılmıştır. Örneklerde mikroskopi ile tespit edilen Candida varlığının,
elektronik burun tarafından da tespit edilip edilemediği
araştırılmıştır. Çalışmada kullanılan elektronik burun
sisteminin var olan teknolojisi ile kök kanallarında var
olan mikroorganizma tipini tespit edemediği ve ileri
teknik donanımlara ihtiyacı olduğu anlaşılmıştır.

Keywords

Elektronik burun, kök kanal enfeksiyonu, mikrobiyoloji, bakteri, mantar

Electronics-Nose of Candida Albicans in Periapical Lesioned Threads Evaluation by Technology and Microbiological Culture Method

Abstract

Most diseases of pulp and periradicular tissues are
associated with mixed micro flora including both
aerobic and anaerobic microorganisms. Clinicians must
understand the close relationship between the presence
of microorganism and endodontic disease process to
determine an effective procedure with respect to the
treatment.
Culture and molecular methods are used to detect
bacterial species in root canal infections. Despite the
recent advances in molecular methods, both culture and
molecular methods have several disadvantages.
The term electronic nose describes an electronic system
that is able to mimic the human sense of smell.
Electronic nose systems have already been used with
success in the medical science and microbiological
research. The most important feature of an electronic
nose system is its ability to detect different odour
types in a short period of time almost with the sensivity
of human nose. Furthermore, the result obtained using
electronic nose system are objective.
In this study, samples collected from root canals were
compared both macroscopically and microscopically
and also with an electronic nose system in order to
detect the presence of candida species in teeth with
periapical lesions. The electronic nose device used in
this study was found insufficient to detect intra canal
candida species and needs more sensitive chemical
sensors.

Keywords

electronic nose, root canal infection, microbiology, bacteria, fungi

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