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PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ

Year 2017, Volume: 26 Issue: 3, 223 - 226, 01.12.2017

Abstract

Pulpa ve periradiküler doku hastalıklarının çoğu hem
aerop hem de anaerop mikroorganizmaları içeren karışık bir mikroflora ile ilişkilidir. Hekim tedavi ile ilgili
etkili bir yöntem belirleyebilmek için mikroorganizma
varlığı ile endodontik hastalık arasındaki yakın ilişkiyi
kavramalıdır.
Endodontik enfeksiyonlardaki bakteri türlerini ortaya
çıkarmak için kültür ve moleküler metodlar kullanılmaktadır. Son yıllarda moleküler yöntemlerdeki gelişmelere rağmen her iki metodun da bir takım dezavantajları bulunmaktadır.
Elektronik burun yöntemi, insan doku duyusunu taklit
edebilen bir elektronik sistemi tanımlar. Elektronik
burun sistemleri tıp alanında ve mikrobiyolojik araştırmalarda başarıyla kullanılmaktadır. Bu sistemlerin en
önemli özelliği, koku çeşitlerini çok kısa zamanda insan
burnundaki hassasiyet derecesinde ayrıştırabilmesi ve
sonucu objektif olarak sunmasıdır.
Bu çalışmada insan diş köklerinden alınan örnekler
elektronik burun sistemine tabi tutulmuş ve
makroskobik ve mikroskobik analizleri yapılmıştır. Örneklerde mikroskopi ile tespit edilen Candida varlığının,
elektronik burun tarafından da tespit edilip edilemediği
araştırılmıştır. Çalışmada kullanılan elektronik burun
sisteminin var olan teknolojisi ile kök kanallarında var
olan mikroorganizma tipini tespit edemediği ve ileri
teknik donanımlara ihtiyacı olduğu anlaşılmıştır.

References

  • 1. Sjögren U, Hänström L, Happonen R, Sundqvist G. Extensive bone loss associated with periapical infection with Bacteroides gingivalis: a case report. Int Endod J 1990;23:254-262.
  • 2. de Oliveira JCM, Siqueira JF, Alves GB, Hirata R, Andrade AF. Detection of Porphyromonas endodontalis in infected root canals by 16S rRNA genedirected polymerase chain reaction. J Endon 2000;26:729-732.
  • 3. Griffee MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW. The relationship ofBacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pathol 1980;50:457- 461.
  • 4. Sundqvist G, Johansson E, Sjögren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod 1989;15:13-19.
  • 5. Odell LJ, Baumgartner JC, Xia T, David LL. Survey for collagenase gene prtC in Porphyromonas gingivalis and Porphyromonas endodontalis isolated from endodontic infections. J Endod 1999;25:555-558.
  • 6. Waltimo T, Siren E, Torkko H, Olsen I, Haapasalo M. Fungi in therapy‐resistant apical periodontitis. Int Endod J 1997;30:96-101.
  • 7. Chávez de Paz L. Gram‐positive organisms in endodontic infections. End Topics 2004;9:79-96.
  • 8. Siqueira JF, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Path Oral Rad Endod 2004;97:632-641.
  • 9. Möller A. Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odont Tids 1966;74:15-19
  • 10. Nair PR, Sjögren U, Krey G, Kahnberg K-E, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 1990;16:580- 588.
  • 11. Tanaka M, Anguri H, Nonaka A, Kataoka K, Nagata H, Kita J, et al. Clinical assessment of oral malodor by the electronic nose system. J Dent Res 2004;83:317- 321.
  • 12. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Rad Endod 1998;85:86-93.
  • 13. Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000;26:695-698.
  • 14. Trihaas J, Vognsen L, Nielsen P. Electronic nose: New tool in modelling the ripening of Danish blue cheese. Int Dairy J 2005;15:679-691.
  • 15. Stuetz R, Fenner R, Engin G. Characterisation of wastewater using an electronic nose. Wat Res 1999;33:442-452.
  • 16. Gardner JW, Shin HW, Hines EL. An electronic nose system to diagnose illness. Sens Act Chemical 2000;70:19-24.
  • 17. Pavlou A, Turner A, Magan N. Recognition of anaerobic bacterial isolates in vitro using electronic nose technology. Letters in Applied Microbiology. 2002;35(5):366-369.
  • 18. Yamada Y, Takahashi Y, Konishi K, Katsuumi I. Association of odor from infected root canal analyzed by an electronic nose with isolated bacteria. J Endod 2007;33:1106-1109.
  • 19. Aksebzeci BH, Asyalı MH, Kahraman Y, Er Ö, Kaya E, Özbilge H, et al. Classification of root canal microorganisms using electronic-nose and discriminant analysis. Biomed Eng Online 2010;9:77.
  • 20.Baumgartner J, Hutter J, Siqueira J. Endodontic microbiology and treatment of infections. Pathways of the Pulp 2006;2:580-607.
  • 21. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Euro J Oral Sci 1981;89:321-328.
  • 22. Dutta R, Hines EL, Gardner JW, Boilot P. Bacteria classification using Cyranose 320 electronic nose. Biomed Eng Online 2002;1:4.
  • 23. Hancock H, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Path Oral Rad Endod 2001;91:579- 586.
  • 24. Cheung G, Ho M. Microbial flora of root canal– treated teeth associated with asymptomatic periapical radiolucent lesions. Molecular Oral Microb 2001;16:332-337.
  • 25. Sen B, Piskin B, Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Dent Trauma 1995;11:6-9.
  • 26. Şen BH, Safavi KE, Spångberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Path Oral Rad Endod 1997;84:68 -73.

Electronics-Nose of Candida Albicans in Periapical Lesioned Threads Evaluation by Technology and Microbiological Culture Method

Year 2017, Volume: 26 Issue: 3, 223 - 226, 01.12.2017

Abstract

Most diseases of pulp and periradicular tissues are
associated with mixed micro flora including both
aerobic and anaerobic microorganisms. Clinicians must
understand the close relationship between the presence
of microorganism and endodontic disease process to
determine an effective procedure with respect to the
treatment.
Culture and molecular methods are used to detect
bacterial species in root canal infections. Despite the
recent advances in molecular methods, both culture and
molecular methods have several disadvantages.
The term electronic nose describes an electronic system
that is able to mimic the human sense of smell.
Electronic nose systems have already been used with
success in the medical science and microbiological
research. The most important feature of an electronic
nose system is its ability to detect different odour
types in a short period of time almost with the sensivity
of human nose. Furthermore, the result obtained using
electronic nose system are objective.
In this study, samples collected from root canals were
compared both macroscopically and microscopically
and also with an electronic nose system in order to
detect the presence of candida species in teeth with
periapical lesions. The electronic nose device used in
this study was found insufficient to detect intra canal
candida species and needs more sensitive chemical
sensors.

References

  • 1. Sjögren U, Hänström L, Happonen R, Sundqvist G. Extensive bone loss associated with periapical infection with Bacteroides gingivalis: a case report. Int Endod J 1990;23:254-262.
  • 2. de Oliveira JCM, Siqueira JF, Alves GB, Hirata R, Andrade AF. Detection of Porphyromonas endodontalis in infected root canals by 16S rRNA genedirected polymerase chain reaction. J Endon 2000;26:729-732.
  • 3. Griffee MB, Patterson SS, Miller CH, Kafrawy AH, Newton CW. The relationship ofBacteroides melaninogenicus to symptoms associated with pulpal necrosis. Oral Surg Oral Med Oral Pathol 1980;50:457- 461.
  • 4. Sundqvist G, Johansson E, Sjögren U. Prevalence of black-pigmented bacteroides species in root canal infections. J Endod 1989;15:13-19.
  • 5. Odell LJ, Baumgartner JC, Xia T, David LL. Survey for collagenase gene prtC in Porphyromonas gingivalis and Porphyromonas endodontalis isolated from endodontic infections. J Endod 1999;25:555-558.
  • 6. Waltimo T, Siren E, Torkko H, Olsen I, Haapasalo M. Fungi in therapy‐resistant apical periodontitis. Int Endod J 1997;30:96-101.
  • 7. Chávez de Paz L. Gram‐positive organisms in endodontic infections. End Topics 2004;9:79-96.
  • 8. Siqueira JF, Sen BH. Fungi in endodontic infections. Oral Surg Oral Med Oral Path Oral Rad Endod 2004;97:632-641.
  • 9. Möller A. Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odont Tids 1966;74:15-19
  • 10. Nair PR, Sjögren U, Krey G, Kahnberg K-E, Sundqvist G. Intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. J Endod 1990;16:580- 588.
  • 11. Tanaka M, Anguri H, Nonaka A, Kataoka K, Nagata H, Kita J, et al. Clinical assessment of oral malodor by the electronic nose system. J Dent Res 2004;83:317- 321.
  • 12. Sundqvist G, Figdor D, Persson S, Sjögren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Rad Endod 1998;85:86-93.
  • 13. Baumgartner JC, Watts CM, Xia T. Occurrence of Candida albicans in infections of endodontic origin. J Endod 2000;26:695-698.
  • 14. Trihaas J, Vognsen L, Nielsen P. Electronic nose: New tool in modelling the ripening of Danish blue cheese. Int Dairy J 2005;15:679-691.
  • 15. Stuetz R, Fenner R, Engin G. Characterisation of wastewater using an electronic nose. Wat Res 1999;33:442-452.
  • 16. Gardner JW, Shin HW, Hines EL. An electronic nose system to diagnose illness. Sens Act Chemical 2000;70:19-24.
  • 17. Pavlou A, Turner A, Magan N. Recognition of anaerobic bacterial isolates in vitro using electronic nose technology. Letters in Applied Microbiology. 2002;35(5):366-369.
  • 18. Yamada Y, Takahashi Y, Konishi K, Katsuumi I. Association of odor from infected root canal analyzed by an electronic nose with isolated bacteria. J Endod 2007;33:1106-1109.
  • 19. Aksebzeci BH, Asyalı MH, Kahraman Y, Er Ö, Kaya E, Özbilge H, et al. Classification of root canal microorganisms using electronic-nose and discriminant analysis. Biomed Eng Online 2010;9:77.
  • 20.Baumgartner J, Hutter J, Siqueira J. Endodontic microbiology and treatment of infections. Pathways of the Pulp 2006;2:580-607.
  • 21. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Euro J Oral Sci 1981;89:321-328.
  • 22. Dutta R, Hines EL, Gardner JW, Boilot P. Bacteria classification using Cyranose 320 electronic nose. Biomed Eng Online 2002;1:4.
  • 23. Hancock H, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Path Oral Rad Endod 2001;91:579- 586.
  • 24. Cheung G, Ho M. Microbial flora of root canal– treated teeth associated with asymptomatic periapical radiolucent lesions. Molecular Oral Microb 2001;16:332-337.
  • 25. Sen B, Piskin B, Demirci T. Observation of bacteria and fungi in infected root canals and dentinal tubules by SEM. Dent Trauma 1995;11:6-9.
  • 26. Şen BH, Safavi KE, Spångberg LS. Growth patterns of Candida albicans in relation to radicular dentin. Oral Surg Oral Med Oral Path Oral Rad Endod 1997;84:68 -73.
There are 26 citations in total.

Details

Other ID JA45CP88TE
Journal Section Research Article
Authors

Özgür Er This is me

Burhan Can Çanakçı This is me

Öznur Tuncay This is me

Yasemin Kahraman This is me

Publication Date December 1, 2017
Submission Date December 1, 2017
Published in Issue Year 2017 Volume: 26 Issue: 3

Cite

APA Er, Ö., Çanakçı, B. C., Tuncay, Ö., Kahraman, Y. (2017). PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ. Sağlık Bilimleri Dergisi, 26(3), 223-226.
AMA Er Ö, Çanakçı BC, Tuncay Ö, Kahraman Y. PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ. JHS. December 2017;26(3):223-226.
Chicago Er, Özgür, Burhan Can Çanakçı, Öznur Tuncay, and Yasemin Kahraman. “PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ”. Sağlık Bilimleri Dergisi 26, no. 3 (December 2017): 223-26.
EndNote Er Ö, Çanakçı BC, Tuncay Ö, Kahraman Y (December 1, 2017) PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ. Sağlık Bilimleri Dergisi 26 3 223–226.
IEEE Ö. Er, B. C. Çanakçı, Ö. Tuncay, and Y. Kahraman, “PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ”, JHS, vol. 26, no. 3, pp. 223–226, 2017.
ISNAD Er, Özgür et al. “PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ”. Sağlık Bilimleri Dergisi 26/3 (December 2017), 223-226.
JAMA Er Ö, Çanakçı BC, Tuncay Ö, Kahraman Y. PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ. JHS. 2017;26:223–226.
MLA Er, Özgür et al. “PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ”. Sağlık Bilimleri Dergisi, vol. 26, no. 3, 2017, pp. 223-6.
Vancouver Er Ö, Çanakçı BC, Tuncay Ö, Kahraman Y. PERİAPİKAL LEZYONLU DİŞLERDE CANDİDA ALBİCANS’İN ELEKTRONİK-BURUN TEKNOLOJİSİ VE MİKROBİYOLOJİK KÜLTÜR YÖNTEMİ İLE DEĞERLENDİRİLMESİ. JHS. 2017;26(3):223-6.