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İSKEMİ-REPERFÜZYON HASARLI RAT OVARYUMUNDA ALBÜMİN UYGULAMASININ SİTOKROM C-1 (CYC1) ÜZERİNE ETKİLERİ

Year 2024, Volume: 33 Issue: 2, 175 - 181, 25.07.2024
https://doi.org/10.34108/eujhs.1345195

Abstract

Bu çalışma, albumin uygulamasının rat ovaryumunda iskemi-reperfüzyon üzerine etkilerini biyokimyasal, histolojik ve immune histokimyasal yöntemlerle incelemeyi amaçladı. Çalışmada 32 adet Wistar albino rat kullanıldı. Hayvanlar 4 gruba ayrıldı: kontrol, albümin, placebo ve iskemi-reperfüzyon. Birinci gruptan sağlıklı overler alındı. Diğer 3 grupta overlere bilateral 2 saatlik iskemi ve 2 saatlik reperfüzyon uygulandı. Albümin grubuna reperfüzyondan 30 dakika önce intraperitoneal albumin (2,5g/kg, %20 human albumin), placebo grubuna reperfüzyondan 30 dakika önce albumin yerine aynı hacimde intraperitoneal salin verildi. Yumurtalık hasar skorları, Sitokrom C-1 immüno reaktivitesi, toplam oksidan durumu, toplam antioksidan durumu ve oksidatif stress indeksi değerleri değerlendirildi. Gruplar arasında yapılan istatistiksel analizlerde toplam oksidan durumu değerleri açısından control grubu sonuçları iskemi-reperfüzyon grubuna gore belirgin olarak düşük olduğu (p=0,001) ve oksidatif stress indeksi değerleri açısından ise iskemi-reperfüzyon grubu sonuçlarının control ve albumin gruplarına gore anlamlı olarak yüksek olduğu görüldü (p<0,001 ve p=0,004, sırasıyla). Histolojik incelemelerde folikül dejenerasyonu, ödem, damar tıkanıklığı ve hemoraji değerlendirilerek elde edilen toplam hasar puanı IR grubunda control grubuna gore anlamlı olarak yüksek bulundu (p=0,003). İmmüno histokimyasal inceleme sonuçlarına gore iskemi-reperfüzyon grubundaki sitokrom C-1 immün reaktivitesinin control ve albumin gruplarına gore anlamlı derecede daha güçlüydü (p<0,001). Albümin uygulamasının sitokrom C-1, oksijen radikalleri ve oksidatif stress düzeylerini düşürdüğünü, bu nedenlei skemi-reperfüzyon tedavi sürecinde yardımcı bir rol oynayacağını düşünüyoruz.

Project Number

2021-TS-93

References

  • Karaçor T, Dogan Z, Elibol E, Bülbül M, Nacar MC. Effects of iloprost on experimental ischemia and reperfusion injury in rat ovary. Biotech Histochem. 2020;95(5):373-380.doi:10.1080/10520295.2019.1703219.
  • Kaplan S, Türk A. Effects of vitamin B12 on rat ovary with ischemia-reperfusion injury. Biotech Histochem. 2022;97(4):284-289.doi:10.1080/10520295.2021.1961863.
  • Toktay E, Tastan TB, Gürbüz MA, et al.Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury. Iran J Basic Med Sci. 2022;25(2):173-178. doi:10.22038/IJBMS.2022.61289.13559.
  • Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H. Effects of apigenin on experimental ischemia/reperfusion injury in the rat ovary. Balkan Med J. 2017;34(5):444-449. doi:10.4274/balkanmedj.2016.1386.
  • Kumar V, Abbas AK, Aster JC (eds): RobbinsBasic Pathology,10th ed. Saunders Publications, c2018:42-48.
  • Galaris D, Barbouti A, Korantzopoulos P. Oxidative stress in hepatic ıschemia-reperfusion ınjury: The role of antioxidants and iron chelating compounds. Curr Pharm Des. 2006;12(23):2875-2890. doi:10.2174/138161206777947614.
  • Toklu H, Deniz M, Yüksel M, Keyer-Uysal M, Şener G. The protective effect of melatonin and amlodipine against cerebral ischemia/reperfusion-induced oxidative brain injury in rats. Marmara Med J. 2009;22(1):34-44.
  • Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Cell biology of ischemia/ reperfusion injury. Int rev cell mol bio. 2012;298(6):229-317.doi: 10.1016/B978-0-12-394309-5.00006-7.
  • Keskin C. CYC1 Geni Transkripsiyonuna Etki Eden Apoptotik Faktörlerin Araştırılması. Uludağ Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Bursa, Türkiye, c2018:1-2.
  • Zhu Y, Li M, Wang X, Jin H, Liu S, Xu J, Chen Q. Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release. Cell Res. 2012;22(1):127-141.doi:10.1038/cr.2011.82.
  • De Simone G, di Masi A, Ascenzi P. Serum albumin: A multifaced enzyme. Int J Mol Sci. 2021;22(18):10086. doi:10.3390/ijms221810086.
  • Sampaio de Holanda G, dos Santos Valença S, Maran Carra A, et al. Sulforaphane and albumin attenuate experimental intestinal ischemia-reperfusion injury. J Surg Res. 2021;262:212-223. doi:10.1016/j.jss.2021.01.014.
  • Schreuder AB, Rice AC, Vanikova J, Vitek L, Shapiro SM, Verkade HJ. Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in gunn rat pumps. Liver Int. 2013;33:1557-1565.doi:10.1111/liv.12219.
  • Çandar T, Uzunlar Ö, Kiseli M, Özcan S. The effect of folate on ischemia/reperfusion injury in a rat adnexal torsion model. Arch Gynecol Obstet. 2021;303(6):1495-1500. doi:10.1007/s00404-020-05934-3.
  • Lempiäinen J, Finckenberg P, Mervaala EE, et al. Dexmedetomidine preconditioning ameliorates kidney ischemia-reperfusion injury. Pharmacol Res Perspect. 2014;2(3):e00045. doi:10.1002/prp2.45.
  • Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The effect of the antioxidant drug “U-74389G” on serum calcium during ischemia reperfusion injury in rats. Pravara Med Rev. 2014;6(2):9-13. doi:10.5835/jecm.omu.31.02.008.
  • Yeral I, Sayan CD, Karaca G, et al. What is the protective effect of krill oil on rat ovary against ischemia–reperfusion injury? J Obstet Gynaecol Res.2019;45(3):592-599.doi:10.1111/jog.13876.
  • Demir M, Yilmaz B, Kalyoncu S, et al. Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress. Taiwan J Obstet Gynecol. 2021;60(1):45-50. doi:10.1016/j.tjog.2020.10.004.
  • Yurtcu E, Togrul C, Ozyer S, et al. Dose dependent protective effects of vardenafil on ischemia-reperfusion injury with biochemical and histopathologic evaluation in rat ovary. J Pediatr Surg. 2015;50(7):1205-1209. doi:10.1016/ j.jpedsurg.2014.12.013.
  • Tokgöz VY, Sipahi M, Keskin O, Guvendi GF, Takir S. Protective effects of vitamin D on ischemia-reperfusion injury of the ovary in a rat model. Iran J Basic Med Sci. 2018;21(6):593-599. doi:10.22038/IJBMS.2018.26914.6581.
  • Taşkın MI, Hismiogullari AA, Yay A, et al.Effect of 2-aminoethoxydiphenyl borate on ischemia-reperfusion injury in a rat ovary model. Eur J ObstetGynecolReprod Biol.2014;178:74–79. doi:10.1016/j.ejogrb.2014.03.049.
  • Dayangan Sayan C, Karaca G, Sema Ozkan Z, et al. What is the protective effect of metformin on rat ovary against ischemia-reperfusion injury? J Obstet Gynaecol Res.2018;44(2):278-285.doi:10.1111/jog.13524.
  • Ersoy Canillioglu Y, Erkanli Senturk G. Alterations of il-1 and VEGF after ischemia-reperfusion injured uterus and ovary in rats. Medeni Med J.2020;35(2):106-115.doi:10.5222/MMJ.2020.67026.
  • Demir Çaltekin M, Özkut MM, Çaltekin İ, et al.The protective effect of JZL184 on ovarian ischemia reperfusion injury and ovarian reserve in rats. J Obstet Gynaecol Res. 2021;47(8):2692-2704. doi:10.1111/jog.14859.
  • Türkler C, Kulhan NG, Ata N, Kiremitli T, Cimen FK, Suleyman H. The ameliorative effect of lutein on ovarian ischemia reperfusion injury in rats. Bratisl Lek Listy. 2018;119(11):713-717. doi:10.4149/BLL_2018_127.
  • Kurek Eken M, Şahin Ersoy G, Kaygusuz EI, et al. Etanercept protects ovarian reserve against ischemia/ reperfusion injury in a rat model. Arch Med Sci. 2019;15(4):1104-1112.doi:10.5114/aoms.2017.72406.
  • Geyikoğlu F, Koc K, Erol HS, et al. The propolis and boric acid can be highly suitable, alone/or as a combinatory approach on ovary ischemia-reperfusion injury. Arch Gynecol Obstet. 2019;300(5):1405-1412. doi:10.1007/s00404-019-05303-9.
  • Çolak S, Koc K, Yıldırım S, Geyikoğlu F. Effects of boric acid on ovarian tissue damage caused by experimental ischemia/reperfusion. Biotech Histochem. 2022;97(6):415-422.doi:10.1080/10520295.2021.2012823.
  • Behroozi-Lak T, Zarei L, Moloody–Tapeh M, Farhad N, Mohammadi R. Protective effects of intraperitoneal administration of nimodipine on ischemia–reperfusion injury in ovaries: Histological and biochemical assessments in a rat model. J Pediatr Surg. 2017;52(4):602-608. doi:10.1016/j.jpedsurg.2016.09.067.
  • Karakaş S, Kaya C, Güraslan H, et al. Effect of metformin and detorsion treatment on serum anti-mullerian hormone levels and ovarian histopathology in a rat ovarian torsion model. Turk J Med Sci. 2020;50(2):455-463. doi:10.3906/sag-1803-196.
  • Yıldırım N, Yigittürk G, Sahingöz Yıldırım AG, et al. Octreotide protects ovary against ischemia-reperfusion injury in rats: Evaluation of histological and biochemical parameters. J Obstet Gynaecol Res. 2015;41(10):1591-1597. doi:10.1111/jog.12770.
  • Yapca OE, Kumbasar S, Salman S, et al.Controlled reperfusion for different durations in the treatment of ischemia-reperfusion injury of the rat ovary: Evaluation of biochemical features, molecular gene expression, and histopathology. Can J Physiol Pharmacol. 2015;93(4):269-274. doi:10.1139/cjpp-2014-0359.
  • Li L, Zhi D, Cheng R, Li J, Luo C, Li H. The neuroprotective role of SIRT1/PGC-1α signaling in limb postconditioning in cerebral ischemia/reperfusion injury. Neurosci. Lett. 2021;749:135736. doi:10.1016/j.neulet.2021.135736.
  • Mølgaard S, Faricelli B, Salomonsson M, Engstrøm T, Treiman M. Increased myocardial vulnerability to ischemia-reperfusion injury in the presence of left ventricular hypertrophy. J Hypertens. 2016;34(3):513-523. doi:10.1097/HJH.0000000000000826.
  • Zhao WP, Wang HW, Liu J, Zhang ZH, Zhu SQ, Zhou BH. Mitochondrial respiratory chain complex abnormal expressions and fusion disorder are involved in fluoride-induced mitochondrial dysfunction in ovarian granulosa cells. Chemosphere.2019;215:619-625. doi:10.1016/j.chemosphere.2018.10.043.

EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY

Year 2024, Volume: 33 Issue: 2, 175 - 181, 25.07.2024
https://doi.org/10.34108/eujhs.1345195

Abstract

This study aimed to examine the effects of albumin administration on ischemia-reperfusion in the rat ovary by using biochemical, histological, and immunohistochemical methods. Thirty-two Wistar albino rats were used in the study, and they were divided into 4 groups: control, albumin, placebo, and ischemia-reperfusion. Healthy ovaries were taken from the first group. In the other three groups, 2-hour ischemia and 2-hour reperfusion were applied to the bilateral ovaries. In the albumin group, intraperitoneal albumin (2.5 g/kg, 20% human albumin) was administered 30 minutes before reperfusion, and in the placebo group, the same volume of intraperitoneal saline was administered instead of albumin 30 minutes before reperfusion. Ovarian damage scores, cytochrome C-1 immunoreactivity, total oxidant status, total antioxidant status, and oxidative stress index levels were evaluated. In the statistical analysis performed between the groups, it was seen that the results of the control group were significantly lower than the ischemia-reperfusion group in terms of total oxidant status values (p=0.001), and the results of the ischemia-reperfusion group were significantly higher than the control and albumin groups in terms of oxidative stress index values (p<0.001 and p=0.004, respectively). In histological examinations, the total damage score obtained by evaluating follicular degeneration, edema, vascular congestion, and hemorrhage was found to be significantly higher in the ischemia-reperfusion group than in the control group (p=0.003). According to the immunohistochemical examination results, cytochrome C-1 immunoreactivity in the ischemia-reperfusion group was significantly stronger than the control and albumin groups (p<0.001). We think that albumin administration reduces cytochrome C-1, reactive oxygen species, and oxidative stress levels, therefore it will play a helpful role in the ischemia-reperfusion treatment process.

Supporting Institution

Kafkas Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2021-TS-93

Thanks

The results described in this article are part of the student thesis (Ali Alper Kahraman's master thesis (2023) at Kafkas University, Institute of Medical Sciences) and this thesis was supported by Kafkas University Scientific Research Projects Coordination Unit (Project No: 2021-TS-93). We would like to thank Dr. Serdar YİĞİT for his contributions to the laboratory processes.

References

  • Karaçor T, Dogan Z, Elibol E, Bülbül M, Nacar MC. Effects of iloprost on experimental ischemia and reperfusion injury in rat ovary. Biotech Histochem. 2020;95(5):373-380.doi:10.1080/10520295.2019.1703219.
  • Kaplan S, Türk A. Effects of vitamin B12 on rat ovary with ischemia-reperfusion injury. Biotech Histochem. 2022;97(4):284-289.doi:10.1080/10520295.2021.1961863.
  • Toktay E, Tastan TB, Gürbüz MA, et al.Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury. Iran J Basic Med Sci. 2022;25(2):173-178. doi:10.22038/IJBMS.2022.61289.13559.
  • Soyman Z, Kelekçi S, Sal V, Şevket O, Bayındır N, Uzun H. Effects of apigenin on experimental ischemia/reperfusion injury in the rat ovary. Balkan Med J. 2017;34(5):444-449. doi:10.4274/balkanmedj.2016.1386.
  • Kumar V, Abbas AK, Aster JC (eds): RobbinsBasic Pathology,10th ed. Saunders Publications, c2018:42-48.
  • Galaris D, Barbouti A, Korantzopoulos P. Oxidative stress in hepatic ıschemia-reperfusion ınjury: The role of antioxidants and iron chelating compounds. Curr Pharm Des. 2006;12(23):2875-2890. doi:10.2174/138161206777947614.
  • Toklu H, Deniz M, Yüksel M, Keyer-Uysal M, Şener G. The protective effect of melatonin and amlodipine against cerebral ischemia/reperfusion-induced oxidative brain injury in rats. Marmara Med J. 2009;22(1):34-44.
  • Kalogeris T, Baines CP, Krenz M, Korthuis RJ. Cell biology of ischemia/ reperfusion injury. Int rev cell mol bio. 2012;298(6):229-317.doi: 10.1016/B978-0-12-394309-5.00006-7.
  • Keskin C. CYC1 Geni Transkripsiyonuna Etki Eden Apoptotik Faktörlerin Araştırılması. Uludağ Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Bursa, Türkiye, c2018:1-2.
  • Zhu Y, Li M, Wang X, Jin H, Liu S, Xu J, Chen Q. Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release. Cell Res. 2012;22(1):127-141.doi:10.1038/cr.2011.82.
  • De Simone G, di Masi A, Ascenzi P. Serum albumin: A multifaced enzyme. Int J Mol Sci. 2021;22(18):10086. doi:10.3390/ijms221810086.
  • Sampaio de Holanda G, dos Santos Valença S, Maran Carra A, et al. Sulforaphane and albumin attenuate experimental intestinal ischemia-reperfusion injury. J Surg Res. 2021;262:212-223. doi:10.1016/j.jss.2021.01.014.
  • Schreuder AB, Rice AC, Vanikova J, Vitek L, Shapiro SM, Verkade HJ. Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in gunn rat pumps. Liver Int. 2013;33:1557-1565.doi:10.1111/liv.12219.
  • Çandar T, Uzunlar Ö, Kiseli M, Özcan S. The effect of folate on ischemia/reperfusion injury in a rat adnexal torsion model. Arch Gynecol Obstet. 2021;303(6):1495-1500. doi:10.1007/s00404-020-05934-3.
  • Lempiäinen J, Finckenberg P, Mervaala EE, et al. Dexmedetomidine preconditioning ameliorates kidney ischemia-reperfusion injury. Pharmacol Res Perspect. 2014;2(3):e00045. doi:10.1002/prp2.45.
  • Tsompos C, Panoulis C, Toutouzas K, Zografos G, Papalois A. The effect of the antioxidant drug “U-74389G” on serum calcium during ischemia reperfusion injury in rats. Pravara Med Rev. 2014;6(2):9-13. doi:10.5835/jecm.omu.31.02.008.
  • Yeral I, Sayan CD, Karaca G, et al. What is the protective effect of krill oil on rat ovary against ischemia–reperfusion injury? J Obstet Gynaecol Res.2019;45(3):592-599.doi:10.1111/jog.13876.
  • Demir M, Yilmaz B, Kalyoncu S, et al. Metformin reduces ovarian ischemia reperfusion injury in rats by improving oxidative/nitrosative stress. Taiwan J Obstet Gynecol. 2021;60(1):45-50. doi:10.1016/j.tjog.2020.10.004.
  • Yurtcu E, Togrul C, Ozyer S, et al. Dose dependent protective effects of vardenafil on ischemia-reperfusion injury with biochemical and histopathologic evaluation in rat ovary. J Pediatr Surg. 2015;50(7):1205-1209. doi:10.1016/ j.jpedsurg.2014.12.013.
  • Tokgöz VY, Sipahi M, Keskin O, Guvendi GF, Takir S. Protective effects of vitamin D on ischemia-reperfusion injury of the ovary in a rat model. Iran J Basic Med Sci. 2018;21(6):593-599. doi:10.22038/IJBMS.2018.26914.6581.
  • Taşkın MI, Hismiogullari AA, Yay A, et al.Effect of 2-aminoethoxydiphenyl borate on ischemia-reperfusion injury in a rat ovary model. Eur J ObstetGynecolReprod Biol.2014;178:74–79. doi:10.1016/j.ejogrb.2014.03.049.
  • Dayangan Sayan C, Karaca G, Sema Ozkan Z, et al. What is the protective effect of metformin on rat ovary against ischemia-reperfusion injury? J Obstet Gynaecol Res.2018;44(2):278-285.doi:10.1111/jog.13524.
  • Ersoy Canillioglu Y, Erkanli Senturk G. Alterations of il-1 and VEGF after ischemia-reperfusion injured uterus and ovary in rats. Medeni Med J.2020;35(2):106-115.doi:10.5222/MMJ.2020.67026.
  • Demir Çaltekin M, Özkut MM, Çaltekin İ, et al.The protective effect of JZL184 on ovarian ischemia reperfusion injury and ovarian reserve in rats. J Obstet Gynaecol Res. 2021;47(8):2692-2704. doi:10.1111/jog.14859.
  • Türkler C, Kulhan NG, Ata N, Kiremitli T, Cimen FK, Suleyman H. The ameliorative effect of lutein on ovarian ischemia reperfusion injury in rats. Bratisl Lek Listy. 2018;119(11):713-717. doi:10.4149/BLL_2018_127.
  • Kurek Eken M, Şahin Ersoy G, Kaygusuz EI, et al. Etanercept protects ovarian reserve against ischemia/ reperfusion injury in a rat model. Arch Med Sci. 2019;15(4):1104-1112.doi:10.5114/aoms.2017.72406.
  • Geyikoğlu F, Koc K, Erol HS, et al. The propolis and boric acid can be highly suitable, alone/or as a combinatory approach on ovary ischemia-reperfusion injury. Arch Gynecol Obstet. 2019;300(5):1405-1412. doi:10.1007/s00404-019-05303-9.
  • Çolak S, Koc K, Yıldırım S, Geyikoğlu F. Effects of boric acid on ovarian tissue damage caused by experimental ischemia/reperfusion. Biotech Histochem. 2022;97(6):415-422.doi:10.1080/10520295.2021.2012823.
  • Behroozi-Lak T, Zarei L, Moloody–Tapeh M, Farhad N, Mohammadi R. Protective effects of intraperitoneal administration of nimodipine on ischemia–reperfusion injury in ovaries: Histological and biochemical assessments in a rat model. J Pediatr Surg. 2017;52(4):602-608. doi:10.1016/j.jpedsurg.2016.09.067.
  • Karakaş S, Kaya C, Güraslan H, et al. Effect of metformin and detorsion treatment on serum anti-mullerian hormone levels and ovarian histopathology in a rat ovarian torsion model. Turk J Med Sci. 2020;50(2):455-463. doi:10.3906/sag-1803-196.
  • Yıldırım N, Yigittürk G, Sahingöz Yıldırım AG, et al. Octreotide protects ovary against ischemia-reperfusion injury in rats: Evaluation of histological and biochemical parameters. J Obstet Gynaecol Res. 2015;41(10):1591-1597. doi:10.1111/jog.12770.
  • Yapca OE, Kumbasar S, Salman S, et al.Controlled reperfusion for different durations in the treatment of ischemia-reperfusion injury of the rat ovary: Evaluation of biochemical features, molecular gene expression, and histopathology. Can J Physiol Pharmacol. 2015;93(4):269-274. doi:10.1139/cjpp-2014-0359.
  • Li L, Zhi D, Cheng R, Li J, Luo C, Li H. The neuroprotective role of SIRT1/PGC-1α signaling in limb postconditioning in cerebral ischemia/reperfusion injury. Neurosci. Lett. 2021;749:135736. doi:10.1016/j.neulet.2021.135736.
  • Mølgaard S, Faricelli B, Salomonsson M, Engstrøm T, Treiman M. Increased myocardial vulnerability to ischemia-reperfusion injury in the presence of left ventricular hypertrophy. J Hypertens. 2016;34(3):513-523. doi:10.1097/HJH.0000000000000826.
  • Zhao WP, Wang HW, Liu J, Zhang ZH, Zhu SQ, Zhou BH. Mitochondrial respiratory chain complex abnormal expressions and fusion disorder are involved in fluoride-induced mitochondrial dysfunction in ovarian granulosa cells. Chemosphere.2019;215:619-625. doi:10.1016/j.chemosphere.2018.10.043.
There are 35 citations in total.

Details

Primary Language English
Subjects Histology and Embryology, Veterinary Histology and Embryology
Journal Section Research Article
Authors

Ali Alper Kahraman 0000-0003-2924-2655

Seyit Ali Bingöl 0000-0001-6422-9582

Project Number 2021-TS-93
Early Pub Date July 22, 2024
Publication Date July 25, 2024
Submission Date August 17, 2023
Published in Issue Year 2024 Volume: 33 Issue: 2

Cite

APA Kahraman, A. A., & Bingöl, S. A. (2024). EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY. Sağlık Bilimleri Dergisi, 33(2), 175-181. https://doi.org/10.34108/eujhs.1345195
AMA Kahraman AA, Bingöl SA. EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY. JHS. July 2024;33(2):175-181. doi:10.34108/eujhs.1345195
Chicago Kahraman, Ali Alper, and Seyit Ali Bingöl. “EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY”. Sağlık Bilimleri Dergisi 33, no. 2 (July 2024): 175-81. https://doi.org/10.34108/eujhs.1345195.
EndNote Kahraman AA, Bingöl SA (July 1, 2024) EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY. Sağlık Bilimleri Dergisi 33 2 175–181.
IEEE A. A. Kahraman and S. A. Bingöl, “EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY”, JHS, vol. 33, no. 2, pp. 175–181, 2024, doi: 10.34108/eujhs.1345195.
ISNAD Kahraman, Ali Alper - Bingöl, Seyit Ali. “EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY”. Sağlık Bilimleri Dergisi 33/2 (July 2024), 175-181. https://doi.org/10.34108/eujhs.1345195.
JAMA Kahraman AA, Bingöl SA. EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY. JHS. 2024;33:175–181.
MLA Kahraman, Ali Alper and Seyit Ali Bingöl. “EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY”. Sağlık Bilimleri Dergisi, vol. 33, no. 2, 2024, pp. 175-81, doi:10.34108/eujhs.1345195.
Vancouver Kahraman AA, Bingöl SA. EFFECTS OF ALBUMIN ADMINISTRATION ON CYTOCHROME C-1 (CYC1) IN ISCHEMIA-REPERFUSION DAMAGED RAT OVARY. JHS. 2024;33(2):175-81.