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Subklinik mastitisli süt ineklerinden izole edilen katalaz negatif Gram pozitif kokların identifikasyonu ve antibiyotiklere duyarlılıkları

Year 2013, Volume: 29 Issue: 3, 153 - 158, 01.09.2013

H. Hüseyin Hadimli Zafer Sayın Osman Erganiş Kürşat Kav

Abstract

Amaç: Bu çalışmada, subklinik mastitisli süt ineklerinin süt örneklerinden izole edilen katalaz negatif Gram pozitif kokların (KNGP) identifikasyonu ve etkenlerin antibiyotiklere duyarlılıklarını belirlenmesi amaçlandı. Gereç ve Yöntem: Subklinik mastitisli süt ineklerinden toplam 104 KNGP kok izole edildi. İzolatlar koloni morfolojisi ve hemoliz özelliklerine göre seçildi ve VİTEC 2 ile identifiye edildi.Bulgular: İzolatların 32’si Enterococcus faecalis (%30.76), 21’i Aerococcus viridans (%20.19), 18’i E. faecium (%17.30), 13’ü Streptococcus agalactiae (%12.50), 11’i S. uberis (%10,57), 3’ü Lactococcus garviae/lactis ssp. lactis (%2,88), 2’si L. lactis ssp. latis (%1.92) ve 1’i L. garviae (%0.96) olarak tanımlandı. Üç izolat (%2,88) ise identifiye edilemedi. KNGP kokların antimikrobiyal ajanlara karşı duyarlılıklarının farklı olduğu tespit edildi. Öneri: Bu çalışma, subklinik mastitisli süt ineklerinden izole edilen KNGP kokların doğru identifikasyonlarının yapılması gerektiğini gösterdi.

Keywords

Gram pozitif kok mastitis süt ineği

References

  • Acar G, Yılmaz E, Solmaz H, Cantekin Z, 2012. Hatay bölgesinde klinik ve subklinik mastitisli ineklerden Streptococcus ssp etkenlerinin izolasyonu ve antibiyotik duyarlılıklarının belirlenmesi. AVKAE Derg, 2, 1-5.
  • Ak S, 2000. Trakya yöresinde sığır mastitislerinden sorumlu bulaşıcı ve çevresel bakteriyel etkenler ve antibiyotiklere duyarlılıkları. İstanbul Üniv Vet Fak Derg, 26, 353-365.
  • Alaçam E, Tekeli T, Erganiş O, İzgi C, 1989. İnek ve mandalarda subklinik mastitislerin tanısı, etkenlerin izolasyonu ve bunlara karşı etkili antibiyotiklerin belirlenmesi. Eurasian J Vet Sci, 5, 81-91.
  • Ateş M, Erganiş O, Çorlu M, Serpek B, 1991. Konya yöresindeki mastitisli ineklerden elde edilen süt örneklerinin mikrobiyel florası ve LDH aktivitesi. Doğa Tr Vet Animal Sci, 47, 152-157.
  • Bramley AJ, Dodd FH, 1984. Reviews of the progres of dairy science: Mastitis control – progress and prospects. J Dairy Res, 51, 481-512.
  • Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F, 1999. Identification of aesculin-hidrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. Vet Microbiol, 70, 87-94.
  • Duarte RS, Mranda OP, Bellei BC, Brito MPVP, Teixeria LM, 2004. Phenotypic and molecular chracteristics of Streptococcus agalactiae isolates recovered from milk of dairy cows in Brazil. J Clin Microbiol, 42, 4214-4222.
  • Ebrahimi A, Nikookhah F, Nikpour S, Majiian F, Gholam M, 2008. Isolation of Streptococci from milk samples of normal, acute and subclinical mastitis cows and determination of their antibiotic susceptibility patterns. Pak J Biol Sci, 11, 148-150.
  • Ergün Y, Aslantaş O, Doğruer G, Cantekin Z, 2004. Hatay ilindeki aile tipi süt sığırcılığı işletmelerinde subklinik mastitislerin epidemiyolojisi. Eurasian J Vet Sci, 20, 25-28.
  • Gordoncillo MJN, Bautista JAN, Hikiba M, Samago IG, Haguingan JMB, 2010. Comparison of conventionally identified mastitis bacterial organisms with commercially available microbial identification kit (BBL Crystal ID). Philipp J Vet Med, 47, 54-57.
  • Haguingan JMB, Gordoncillo MJN, Bautista JAN, Hikib M, Samago IG, 2010. Detection and identification of bacterial carriage of subclinical mastitis cases in backyard dairy cows. Philipp J Vet Med, 47, 21-25.
  • Heleili N, Ayachi A, Melizi M, Kassah AL, Mamache B, 2012. Prevalence of subclinical bovine mastitis and the in vitro sensitivity of bacterial isolates in Batna Governorate, East of Algeria. J Anim Sci Adv, 26, 576-582.
  • Leigh JA, 1999. Streptococcus uberis: A permanent barrier to the control of bovine mastitis? Vet J, 157, 225-238.
  • Karahan M, 2005. Mastitisli inek sütlerinden izole edilen streptokok ve stafilokok etkenlerinde genetik polimorfizmin araştırılması. Fırat Üniversitesi, Sağlık Bilimleri Enstitüsü, Doktora Tezi, Elazığ, Türkiye.
  • Klimiene I, Ruzauskas M, Spakauskas V, Mocheliunas R, Pereckiene A, Butrimaite-Ambrozeviciene C, 2011. Prevalence of Gram positive bacteria in cow mastitis and their susceptibiliy to beta-lactam antibiotics. Vet Med Zoot, 56, 65-72.
  • Macun HC, Pir Yağcı İ, Ünal N, Kalender H, Skarya F, Yıldırım M, 2011. Kırıkkale’de belirlenen subklinik mastitisli ineklerde etken izolasyonu ve antibiyotik direnç durumu. Erciyes Üniv Vet Fak Derg, 8, 83-89.
  • Maricato E, Lange CC, Brito MAVP, Brito JRF, Cerqueria MMOP, 2005. Chracterization and antibiotic susceptibility patterns of Catalase Negative Gram Positive Cocci isolated from bovine mastitis in Brazil. ISAH 2005, Warsaw, Poland, pp: 54-55
  • Meiri-Bendek I, Lipkin A, Friedmann A, 2001. A PCR-based method for the detection of Streptococcus agalactiae in milk. J Dairy Sci, 85, 1717-1723.
  • Merl K, Abdulmawjood A, Lammmler C, Zschock M, 2003. Determination of epidemiological relationship of Streptococcus agalactiae isolated from bovine mastitis. FEMS Microbiol Letters, 226, 87-92.
  • Metzger S, Hogan J, 2008. Biofilm formation by Enterococcus species of bovine mammary gland and environmental origins. The Ohio State University. Department of Animal Sciences Honors Theses, USA.
  • NCCLS, 2003. National committee for clinical laboratory standards: Performance standards for antimicrobial susceptibility testing. Eighth Edition, NCCLS document M2-A8 volume 23, no 1, pp 22-23.
  • Nyman AK, 2007. Epidemiological studies of risk factors for bovine mastitis. Doctoral thesis, Swedish University of Agricultural Sciences, Swedish.
  • Osteras O, Solverod L, Reksen O, 2006. Milk culture results in a large Norwegian survey-effects of season, parity, days in milk, resistance, and clustering. J Dairy Sci, 89, 1010-1023.
  • Rossitto RV, Ruiz L, Kikuchi Y, Gleen K, Luiz K, Watts JL, Cullor JS, 2002. Antibiotic susceptibility patterns for environmental streptococci isolated from bovine mastitis in Central California dairies. J Dairy Sci, 85, 132-138.
  • Shetty N, Hill G, Ridgway GL, 1998. Witek analyser for routine bacterial identification and susceptibility testing:Protocols, problems and pitfalls. J Clin Pathol, 51, 316-323.
  • Spakova T, Elecko J, Vasil M, Legath J, Priston P, Javorsk P, 2012. Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia. Pol J Vet Sci, 15, 329-335.
  • Wallet F, Loiez C, Renaux E, Lemaitre N, Courcol RJ, 2005. Performances of VITEK 2 colorimetric cards for identification of Gram positive and Gram negative bacteria. J Clin Microbiol, 43, 4402-4406.
  • Walther C, Rossano A, Thomann A, Perreten V, 2008. Antibiotic resistance in Lactococcus species from bovine milk:Presence of a mutated multidrug transporter mdt(A) gene in susceptible Lactococcus garvieae strains. Vet Microbiol, 131, 348-357.
  • Wyder AB, Boss R, Naskova J, Kaufmann T, Steiner A, Graber HU, 2011. Streptococcus ssp. and related bacteria: Their identification and their pathogenic potential for chronic mastitis-A molecular approach. Res Vet Sci, 91, 349-357

Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis

Year 2013, Volume: 29 Issue: 3, 153 - 158, 01.09.2013

H. Hüseyin Hadimli Zafer Sayın Osman Erganiş Kürşat Kav

Abstract

Aim: This study was aimed to identify of catalase negative Gram positive cocci (CNGP) isolated from milk samples of dairy cows with subclinical mastitis and to determine susceptibility of microorganisms to antibiotics. Materials and Method: Totally, 104 CNGP cocci were isolated from dairy cows with subclinical mastitis. Isolates were selected according to coloni morphological and haemolysis properties and were identified by VITEC 2. Results: Out of isolates, 32 (30.76%) were identified as Enterococcus faecalis, 21 (20.19%) Aerococcus viridans, 18 (17.30%) E. faecium, 13 (12.50%) Streptococcus agalactiae, 11 (10.57%) S. uberis, 3 (2.88%) Lactococcus garviae/lactis ssp. lactis, 2 (1.92%) L. lactis ssp. latis and 1 (0.96%) L. garviae. Three isolates (2.88%) could not be identified. The susceptibilities of CNGP cocci were determined to be different to antimicrobial agents. Conclusion: This study shown that identification of CNGP cocci isolated from dairy cows with subclinical mastitis should be performed accordingly.

Keywords

Gram positive cocci mastitis dairy cow

References

  • Acar G, Yılmaz E, Solmaz H, Cantekin Z, 2012. Hatay bölgesinde klinik ve subklinik mastitisli ineklerden Streptococcus ssp etkenlerinin izolasyonu ve antibiyotik duyarlılıklarının belirlenmesi. AVKAE Derg, 2, 1-5.
  • Ak S, 2000. Trakya yöresinde sığır mastitislerinden sorumlu bulaşıcı ve çevresel bakteriyel etkenler ve antibiyotiklere duyarlılıkları. İstanbul Üniv Vet Fak Derg, 26, 353-365.
  • Alaçam E, Tekeli T, Erganiş O, İzgi C, 1989. İnek ve mandalarda subklinik mastitislerin tanısı, etkenlerin izolasyonu ve bunlara karşı etkili antibiyotiklerin belirlenmesi. Eurasian J Vet Sci, 5, 81-91.
  • Ateş M, Erganiş O, Çorlu M, Serpek B, 1991. Konya yöresindeki mastitisli ineklerden elde edilen süt örneklerinin mikrobiyel florası ve LDH aktivitesi. Doğa Tr Vet Animal Sci, 47, 152-157.
  • Bramley AJ, Dodd FH, 1984. Reviews of the progres of dairy science: Mastitis control – progress and prospects. J Dairy Res, 51, 481-512.
  • Devriese LA, Hommez J, Laevens H, Pot B, Vandamme P, Haesebrouck F, 1999. Identification of aesculin-hidrolyzing streptococci, lactococci, aerococci and enterococci from subclinical intramammary infections in dairy cows. Vet Microbiol, 70, 87-94.
  • Duarte RS, Mranda OP, Bellei BC, Brito MPVP, Teixeria LM, 2004. Phenotypic and molecular chracteristics of Streptococcus agalactiae isolates recovered from milk of dairy cows in Brazil. J Clin Microbiol, 42, 4214-4222.
  • Ebrahimi A, Nikookhah F, Nikpour S, Majiian F, Gholam M, 2008. Isolation of Streptococci from milk samples of normal, acute and subclinical mastitis cows and determination of their antibiotic susceptibility patterns. Pak J Biol Sci, 11, 148-150.
  • Ergün Y, Aslantaş O, Doğruer G, Cantekin Z, 2004. Hatay ilindeki aile tipi süt sığırcılığı işletmelerinde subklinik mastitislerin epidemiyolojisi. Eurasian J Vet Sci, 20, 25-28.
  • Gordoncillo MJN, Bautista JAN, Hikiba M, Samago IG, Haguingan JMB, 2010. Comparison of conventionally identified mastitis bacterial organisms with commercially available microbial identification kit (BBL Crystal ID). Philipp J Vet Med, 47, 54-57.
  • Haguingan JMB, Gordoncillo MJN, Bautista JAN, Hikib M, Samago IG, 2010. Detection and identification of bacterial carriage of subclinical mastitis cases in backyard dairy cows. Philipp J Vet Med, 47, 21-25.
  • Heleili N, Ayachi A, Melizi M, Kassah AL, Mamache B, 2012. Prevalence of subclinical bovine mastitis and the in vitro sensitivity of bacterial isolates in Batna Governorate, East of Algeria. J Anim Sci Adv, 26, 576-582.
  • Leigh JA, 1999. Streptococcus uberis: A permanent barrier to the control of bovine mastitis? Vet J, 157, 225-238.
  • Karahan M, 2005. Mastitisli inek sütlerinden izole edilen streptokok ve stafilokok etkenlerinde genetik polimorfizmin araştırılması. Fırat Üniversitesi, Sağlık Bilimleri Enstitüsü, Doktora Tezi, Elazığ, Türkiye.
  • Klimiene I, Ruzauskas M, Spakauskas V, Mocheliunas R, Pereckiene A, Butrimaite-Ambrozeviciene C, 2011. Prevalence of Gram positive bacteria in cow mastitis and their susceptibiliy to beta-lactam antibiotics. Vet Med Zoot, 56, 65-72.
  • Macun HC, Pir Yağcı İ, Ünal N, Kalender H, Skarya F, Yıldırım M, 2011. Kırıkkale’de belirlenen subklinik mastitisli ineklerde etken izolasyonu ve antibiyotik direnç durumu. Erciyes Üniv Vet Fak Derg, 8, 83-89.
  • Maricato E, Lange CC, Brito MAVP, Brito JRF, Cerqueria MMOP, 2005. Chracterization and antibiotic susceptibility patterns of Catalase Negative Gram Positive Cocci isolated from bovine mastitis in Brazil. ISAH 2005, Warsaw, Poland, pp: 54-55
  • Meiri-Bendek I, Lipkin A, Friedmann A, 2001. A PCR-based method for the detection of Streptococcus agalactiae in milk. J Dairy Sci, 85, 1717-1723.
  • Merl K, Abdulmawjood A, Lammmler C, Zschock M, 2003. Determination of epidemiological relationship of Streptococcus agalactiae isolated from bovine mastitis. FEMS Microbiol Letters, 226, 87-92.
  • Metzger S, Hogan J, 2008. Biofilm formation by Enterococcus species of bovine mammary gland and environmental origins. The Ohio State University. Department of Animal Sciences Honors Theses, USA.
  • NCCLS, 2003. National committee for clinical laboratory standards: Performance standards for antimicrobial susceptibility testing. Eighth Edition, NCCLS document M2-A8 volume 23, no 1, pp 22-23.
  • Nyman AK, 2007. Epidemiological studies of risk factors for bovine mastitis. Doctoral thesis, Swedish University of Agricultural Sciences, Swedish.
  • Osteras O, Solverod L, Reksen O, 2006. Milk culture results in a large Norwegian survey-effects of season, parity, days in milk, resistance, and clustering. J Dairy Sci, 89, 1010-1023.
  • Rossitto RV, Ruiz L, Kikuchi Y, Gleen K, Luiz K, Watts JL, Cullor JS, 2002. Antibiotic susceptibility patterns for environmental streptococci isolated from bovine mastitis in Central California dairies. J Dairy Sci, 85, 132-138.
  • Shetty N, Hill G, Ridgway GL, 1998. Witek analyser for routine bacterial identification and susceptibility testing:Protocols, problems and pitfalls. J Clin Pathol, 51, 316-323.
  • Spakova T, Elecko J, Vasil M, Legath J, Priston P, Javorsk P, 2012. Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia. Pol J Vet Sci, 15, 329-335.
  • Wallet F, Loiez C, Renaux E, Lemaitre N, Courcol RJ, 2005. Performances of VITEK 2 colorimetric cards for identification of Gram positive and Gram negative bacteria. J Clin Microbiol, 43, 4402-4406.
  • Walther C, Rossano A, Thomann A, Perreten V, 2008. Antibiotic resistance in Lactococcus species from bovine milk:Presence of a mutated multidrug transporter mdt(A) gene in susceptible Lactococcus garvieae strains. Vet Microbiol, 131, 348-357.
  • Wyder AB, Boss R, Naskova J, Kaufmann T, Steiner A, Graber HU, 2011. Streptococcus ssp. and related bacteria: Their identification and their pathogenic potential for chronic mastitis-A molecular approach. Res Vet Sci, 91, 349-357
There are 29 citations in total.

Details

Other ID JA69GC28RV
Journal Section Research
Authors

H. Hüseyin Hadimli This is me

Zafer Sayın This is me

Osman Erganiş This is me

Kürşat Kav This is me

Publication Date September 1, 2013
Published in Issue Year 2013 Volume: 29 Issue: 3

Cite

APA Hadimli, H. H., Sayın, Z., Erganiş, O., Kav, K. (2013). Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian Journal of Veterinary Sciences, 29(3), 153-158.
AMA Hadimli HH, Sayın Z, Erganiş O, Kav K. Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian J Vet Sci. September 2013;29(3):153-158.
Chicago Hadimli, H. Hüseyin, Zafer Sayın, Osman Erganiş, and Kürşat Kav. “Identification and Antibiotic Susceptibility of Catalase Negative Gram Positive Cocci Isolated from Dairy Cows With Subclinical Mastitis”. Eurasian Journal of Veterinary Sciences 29, no. 3 (September 2013): 153-58.
EndNote Hadimli HH, Sayın Z, Erganiş O, Kav K (September 1, 2013) Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian Journal of Veterinary Sciences 29 3 153–158.
IEEE H. H. Hadimli, Z. Sayın, O. Erganiş, and K. Kav, “Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis”, Eurasian J Vet Sci, vol. 29, no. 3, pp. 153–158, 2013.
ISNAD Hadimli, H. Hüseyin et al. “Identification and Antibiotic Susceptibility of Catalase Negative Gram Positive Cocci Isolated from Dairy Cows With Subclinical Mastitis”. Eurasian Journal of Veterinary Sciences 29/3 (September 2013), 153-158.
JAMA Hadimli HH, Sayın Z, Erganiş O, Kav K. Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian J Vet Sci. 2013;29:153–158.
MLA Hadimli, H. Hüseyin et al. “Identification and Antibiotic Susceptibility of Catalase Negative Gram Positive Cocci Isolated from Dairy Cows With Subclinical Mastitis”. Eurasian Journal of Veterinary Sciences, vol. 29, no. 3, 2013, pp. 153-8.
Vancouver Hadimli HH, Sayın Z, Erganiş O, Kav K. Identification and antibiotic susceptibility of catalase negative Gram positive cocci isolated from dairy cows with subclinical mastitis. Eurasian J Vet Sci. 2013;29(3):153-8.