17β östradiolün ovariektomize rat uterusunda eNOS ve iNOS ekspresyonları üzerine etkisi

Year 2013, Volume: 29 Issue: 2, 65 - 69, 01.06.2013

Oktay Yilmaz Artay Yagci Elmas Ulutas Alper Sevimli Korhan Altunbas Aziz Bulbul Recep Aslan

Abstract

Amaç: Bu çalışmada ovariektomize rat uterusunda 17β östradiolün doza bağımlı olarak endoteliyal nitrik oksit sentaz (eNOS) ve indüklenebilir NOS (iNOS) enzimlerinin ekspresyon modelleri üzerine etkisinin Western blot yöntemiyle ortaya konulması amaçlandı.Gereç ve Yöntem: Sprague Dawley ırkı, ovariektomize edilmiş, 3 aylık 40 adet dişi rat kullanıldı. Kontrol grubundaki ratlar (n=10) kasiçi, 3 gün boyunca susam yağı alırken, deneme grubundaki ratlara 3 gün boyunca 25 (n=10), 50 (n=10) ve 100 (n=10) µg/rat/gün dozlarında kas içi 17β östradiol uygulandı. Son uygulamadan 18 saat sonra ratlara servikal dislokasyon uygulandı. Uterus örnekleri vakit kaybetmeden uzaklaştırıldı. Uterus örneklerindeki eNOS ve iNOS enzimlerinin varlığı Western blot ile analiz edilerek elde edilen filmlerde dansitometri gerçekleştirildi. Bulgular: 25, 50 ve 100 µg/rat/gün 17β östradiol uygulamalarının eNOS ekspiresyonunu kontrol grubuna göre arttırdığı gözlendi. 25 ve 50 µg/rat/gün 17β östradiol gruplarındaki eNOS exspiresyonu relatif dansitesinin 100 µg/rat/gün 17β östradiol grubuna göre yüksek olduğu belirlendi. eNOS’a benzer şekilde, 25 ve 50 µg/rat/gün 17β östradiol gruplarındaki iNOS ekspiresyonunun kontrol ve 100 µg/rat/gün 17β östradiol gruplarına göre yüksek olduğu izlendi. Öneri: Östrojenin NOS/NO aktivitesine aracılık edebileceği ileri
sürülebilir.

Keywords

17β östradiol, eNOS, iNOS, rat uterus, ovariektomi

The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus

Abstract

Aim: This study was design to describe the effect of 17β estradiol on the dose-dependent expression patterns of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) enzymes by Western blotting in ovariectomized rat uterus. Materials and Methods: Female, three months old, ovariectomized, 40 Sprague–Dawley rats were used. Rats in the control group (n=10) received intramuscular injection of sesame oil once daily for 3 days, whereas rats in the experimental groups were treated with intramuscular injection of 17β estradiol 25 (n=10), 50 (n=10) and 100 (n=10) µg/rat/day. The rats were killed by cervical dislocation at 18th hour after the last injection. Immediately after death, the uterine horns were removed. The presence of eNOS and iNOS enzymes in uterine samples were analysed by Western blot and the densitometry of each film were performed. Results: It was observed that the application of 25, 50 and 100 µg/rat/day 17β estradiol increased the eNOS expression as compared to control group The relative density of eNOS expressions in 25 and 50 µg/rat/day 17β estradiol groups was higher than those in 100 µg/rat/day 17β estradiol group. Similarly to eNOS, 25 and 50 µg/rat/day 17β estradiol groups showed higher iNOS expression as compared to control and 100 µg/rat/day 17β estradiol groups.Conclusion: Estrogen may mediate the NOS/NO activity in ovariectomized rat uterus

Keywords

17β estradiol, eNOS, iNOS, rat uterus, ovariectomy

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