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17β östradiolün ovariektomize rat uterusunda eNOS ve iNOS ekspresyonları üzerine etkisi

Yıl 2013, Cilt: 29 Sayı: 2, 65 - 69, 01.06.2013

Öz

Amaç: Bu çalışmada ovariektomize rat uterusunda 17β östradiolün doza bağımlı olarak endoteliyal nitrik oksit sentaz (eNOS) ve indüklenebilir NOS (iNOS) enzimlerinin ekspresyon modelleri üzerine etkisinin Western blot yöntemiyle ortaya konulması amaçlandı.Gereç ve Yöntem: Sprague Dawley ırkı, ovariektomize edilmiş, 3 aylık 40 adet dişi rat kullanıldı. Kontrol grubundaki ratlar (n=10) kasiçi, 3 gün boyunca susam yağı alırken, deneme grubundaki ratlara 3 gün boyunca 25 (n=10), 50 (n=10) ve 100 (n=10) µg/rat/gün dozlarında kas içi 17β östradiol uygulandı. Son uygulamadan 18 saat sonra ratlara servikal dislokasyon uygulandı. Uterus örnekleri vakit kaybetmeden uzaklaştırıldı. Uterus örneklerindeki eNOS ve iNOS enzimlerinin varlığı Western blot ile analiz edilerek elde edilen filmlerde dansitometri gerçekleştirildi. Bulgular: 25, 50 ve 100 µg/rat/gün 17β östradiol uygulamalarının eNOS ekspiresyonunu kontrol grubuna göre arttırdığı gözlendi. 25 ve 50 µg/rat/gün 17β östradiol gruplarındaki eNOS exspiresyonu relatif dansitesinin 100 µg/rat/gün 17β östradiol grubuna göre yüksek olduğu belirlendi. eNOS’a benzer şekilde, 25 ve 50 µg/rat/gün 17β östradiol gruplarındaki iNOS ekspiresyonunun kontrol ve 100 µg/rat/gün 17β östradiol gruplarına göre yüksek olduğu izlendi. Öneri: Östrojenin NOS/NO aktivitesine aracılık edebileceği ileri
sürülebilir.

Kaynakça

  • Azevedo RB, Lacava ZGM, Miyasaka CK, Chaves CB, Curi R, 2001. Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids. Brazilian J Med Biol Res, 34, 683-687.
  • Batra S, Al-Hajji J, 1998. Characterization of nitric oxide synthase activity in rabbit uterus and vagina: Downregulation by estrogen. Life Sci, 62, 2093-2100.
  • Bulbul A, Yagci A, Altunbas K, Sevimli A, Celik HA, Karadeniz A, Akdag E, 2007. The role of nitric oxide in the effects of ovarian steroids on spontaneous myometrial contractility in rats. Theriogenology, 68, 1156-68.
  • Chatterjee S, Gangula PR, Dong YL, Yallampali C, 1996. Immunocytochemical localization of nitric oxide synthase-III in reproductive organs of female rats during the oestrus cycle. Histochemistry, 28, 715-723.
  • Farina M, Ribeiro ML, Franchi A, 2001. Nitric oxide synthase in pregnant rat uterus. Reproduction, 121, 403-407.
  • Figueroa JP, Massmann GA, 1995. Estrogen increases nitric oxide synthase activity in the uterus of nonpregnant sheep. Am J Obstet Gynecol, 173, 1539-1545.
  • Förstermann U, Schmidt HHW, Pollock JS, Sheng H, Mitchell JA, Warner TD, et al, 1991. Isoforms of nitric oxide synthase. Characterization and purification from different cell types. Biochem Pharmacol, 42, 1849-1857.
  • Förstermann U, Boissel JP, Kleinert H, 1998. Expressional control of the “constitutive” isoforms of nitric oxide synthase (NOS I and nos III). FASEB J, 12, 773-790.
  • Gangula PRR, Dong YL, Yallampalli C, 1997. Rat myometrial smooth muscle cells express nitric oxide synthase. Hum Reprod, 12, 561-568.
  • Huang J, Roby KF, Pace JL, Russell SW, Hunt JS, 1995. Cellular localization and hormonal regulation of inducible nitric oxide synthase in cycling mouse uterus. J Leukocyte Biol, 57, 27-35.
  • Jaroszewski JJ, Skarzynski DJ, Okuda K, 2001. Nitric oxide as a local regulator in the mammalian ovary. In: Reproductive Biotechnology: Reproductive Biotechnology Update and Its Related Physiology, Eds; Miyamoto H, Manabe N, Tokyo, Elsevier Scientific, Japan, pp: 105-112.
  • Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Maul H, Longo M, Saade GR, Garfield RE, 2003. Nitric oxide and its role during pregnancy: From ovulation to delivery. Curr Pharma Des, 9, 359-380.
  • Miura T, Muraoka S, Ogiso T, 1996. Inhibition of lipid peroxidation by estradiol and 2-hydroxyestradiol. Steroids, 61, 379-383.
  • Nelson SH, Steinsland OS, Wang Y, Yallampalli C, Dong YL, Sanchez JM, 2000. Increased nitric oxide synthase activity and expression in the human uterine artery during pregnancy. Circul Res, 87, 406-411.
  • Ogando D, Farina M, Ribeiro Ml, Perez Martinez S, Cella M, Rettori V, Franchi C, 2003. Steroids hormones augment nitric oxide synthase activity and expression in rat uterus. Reprod Fertil Dev, 15, 269-274.
  • Ota H, Garashi S, Hatazawa J, Tanaka T, 1998. Endothelial nitric oxide synthase in the endometrium during the menstrual cycle in patients with endometriosis and adenomyosis. Fertil Steril, 69, 303-308.
  • Palmer RM, Ashton DS, Moncada S, 1988. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature, 333, 664-666.
  • Rosselli M, Keller PJ, Dubey RK, 1998. Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum Reprod, 4, 3-24.
  • Rossmanith WG, Hoffmeister U, Wolfarth S, Kleine B, Mclean M, Jacobs RA, Grossman AB, 1999. Expression and functional analysis of endothelial nitric oxide synthase (eNOS) in human placenta. Mol Hum Reprod, 5, 487-494.
  • Saxena D, Purohit SB, Kumar PG, Laloroya M, 2000. Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation. Nitric Oxide, 4, 384-394.
  • Taguchi M, Alfer J, Chwalisz K, Beier HM, Classen LI, 2000. Endothelial nitric oxide synthase is differently expressed in human endometrial vessels during the menstrual cycle. Mol Hum Reprod, 6, 185-190.
  • Vagnoni KE, Shaw CE, Phernetton TM, Meglin BM, Bird IM, Magness RR, 1998. Endothelial vasodilator production by uterine and systemic arteries. III. Ovarian and estrogen effects on NO synthase. Am J Physiol, 275, 1845-1856.
  • Weiner CR, Lizasoain I, Baylis SA, Knowles RG, Charles IG, Moncada S, 1994. Induction of calcium-dependent nitric oxide synthases by sex hormones. Proc Natl Acad Sci USA, 91, 5212- 5216.
  • Yallampalli C, Dong YL, 2000. Estradiol-17b inhibits nitric oxide synthase (NOS)-II and stimulates NOS-III gene expression in the rat uterus. Biol Reprod, 63, 34-41.
  • Yallampalli C, Dong YL, Gangula PR, Fong L, 1998. Role and regulation of nitric oxide in the uterus during pregnancy and parturition. J Soc Gynecol Invest, 5, 58-67.
  • Yilmaz O, Calka J, Bukowski R, Zalecki M, Wasowicz K, Jaroszewski JJ, Markiewicz W, Bulbul A, Ucar M, 2012. Nitric oxide in the bovine oviduct: Influence on contractile activity and nitric oxide synthase isoforms localization. Theriogenology, 77, 1312-1327.
  • Zhang J, Massmann GA, Mirabile CP, Figueroa JP, 1999. Non-pregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. Biol Reprod, 60, 1198-1203

The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus

Yıl 2013, Cilt: 29 Sayı: 2, 65 - 69, 01.06.2013

Öz

Aim: This study was design to describe the effect of 17β estradiol on the dose-dependent expression patterns of endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS) enzymes by Western blotting in ovariectomized rat uterus. Materials and Methods: Female, three months old, ovariectomized, 40 Sprague–Dawley rats were used. Rats in the control group (n=10) received intramuscular injection of sesame oil once daily for 3 days, whereas rats in the experimental groups were treated with intramuscular injection of 17β estradiol 25 (n=10), 50 (n=10) and 100 (n=10) µg/rat/day. The rats were killed by cervical dislocation at 18th hour after the last injection. Immediately after death, the uterine horns were removed. The presence of eNOS and iNOS enzymes in uterine samples were analysed by Western blot and the densitometry of each film were performed. Results: It was observed that the application of 25, 50 and 100 µg/rat/day 17β estradiol increased the eNOS expression as compared to control group The relative density of eNOS expressions in 25 and 50 µg/rat/day 17β estradiol groups was higher than those in 100 µg/rat/day 17β estradiol group. Similarly to eNOS, 25 and 50 µg/rat/day 17β estradiol groups showed higher iNOS expression as compared to control and 100 µg/rat/day 17β estradiol groups.Conclusion: Estrogen may mediate the NOS/NO activity in ovariectomized rat uterus

Kaynakça

  • Azevedo RB, Lacava ZGM, Miyasaka CK, Chaves CB, Curi R, 2001. Regulation of antioxidant enzyme activities in male and female rat macrophages by sex steroids. Brazilian J Med Biol Res, 34, 683-687.
  • Batra S, Al-Hajji J, 1998. Characterization of nitric oxide synthase activity in rabbit uterus and vagina: Downregulation by estrogen. Life Sci, 62, 2093-2100.
  • Bulbul A, Yagci A, Altunbas K, Sevimli A, Celik HA, Karadeniz A, Akdag E, 2007. The role of nitric oxide in the effects of ovarian steroids on spontaneous myometrial contractility in rats. Theriogenology, 68, 1156-68.
  • Chatterjee S, Gangula PR, Dong YL, Yallampali C, 1996. Immunocytochemical localization of nitric oxide synthase-III in reproductive organs of female rats during the oestrus cycle. Histochemistry, 28, 715-723.
  • Farina M, Ribeiro ML, Franchi A, 2001. Nitric oxide synthase in pregnant rat uterus. Reproduction, 121, 403-407.
  • Figueroa JP, Massmann GA, 1995. Estrogen increases nitric oxide synthase activity in the uterus of nonpregnant sheep. Am J Obstet Gynecol, 173, 1539-1545.
  • Förstermann U, Schmidt HHW, Pollock JS, Sheng H, Mitchell JA, Warner TD, et al, 1991. Isoforms of nitric oxide synthase. Characterization and purification from different cell types. Biochem Pharmacol, 42, 1849-1857.
  • Förstermann U, Boissel JP, Kleinert H, 1998. Expressional control of the “constitutive” isoforms of nitric oxide synthase (NOS I and nos III). FASEB J, 12, 773-790.
  • Gangula PRR, Dong YL, Yallampalli C, 1997. Rat myometrial smooth muscle cells express nitric oxide synthase. Hum Reprod, 12, 561-568.
  • Huang J, Roby KF, Pace JL, Russell SW, Hunt JS, 1995. Cellular localization and hormonal regulation of inducible nitric oxide synthase in cycling mouse uterus. J Leukocyte Biol, 57, 27-35.
  • Jaroszewski JJ, Skarzynski DJ, Okuda K, 2001. Nitric oxide as a local regulator in the mammalian ovary. In: Reproductive Biotechnology: Reproductive Biotechnology Update and Its Related Physiology, Eds; Miyamoto H, Manabe N, Tokyo, Elsevier Scientific, Japan, pp: 105-112.
  • Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
  • Maul H, Longo M, Saade GR, Garfield RE, 2003. Nitric oxide and its role during pregnancy: From ovulation to delivery. Curr Pharma Des, 9, 359-380.
  • Miura T, Muraoka S, Ogiso T, 1996. Inhibition of lipid peroxidation by estradiol and 2-hydroxyestradiol. Steroids, 61, 379-383.
  • Nelson SH, Steinsland OS, Wang Y, Yallampalli C, Dong YL, Sanchez JM, 2000. Increased nitric oxide synthase activity and expression in the human uterine artery during pregnancy. Circul Res, 87, 406-411.
  • Ogando D, Farina M, Ribeiro Ml, Perez Martinez S, Cella M, Rettori V, Franchi C, 2003. Steroids hormones augment nitric oxide synthase activity and expression in rat uterus. Reprod Fertil Dev, 15, 269-274.
  • Ota H, Garashi S, Hatazawa J, Tanaka T, 1998. Endothelial nitric oxide synthase in the endometrium during the menstrual cycle in patients with endometriosis and adenomyosis. Fertil Steril, 69, 303-308.
  • Palmer RM, Ashton DS, Moncada S, 1988. Vascular endothelial cells synthesize nitric oxide from L-arginine. Nature, 333, 664-666.
  • Rosselli M, Keller PJ, Dubey RK, 1998. Role of nitric oxide in the biology, physiology and pathophysiology of reproduction. Hum Reprod, 4, 3-24.
  • Rossmanith WG, Hoffmeister U, Wolfarth S, Kleine B, Mclean M, Jacobs RA, Grossman AB, 1999. Expression and functional analysis of endothelial nitric oxide synthase (eNOS) in human placenta. Mol Hum Reprod, 5, 487-494.
  • Saxena D, Purohit SB, Kumar PG, Laloroya M, 2000. Increased appearance of inducible nitric oxide synthase in the uterus and embryo at implantation. Nitric Oxide, 4, 384-394.
  • Taguchi M, Alfer J, Chwalisz K, Beier HM, Classen LI, 2000. Endothelial nitric oxide synthase is differently expressed in human endometrial vessels during the menstrual cycle. Mol Hum Reprod, 6, 185-190.
  • Vagnoni KE, Shaw CE, Phernetton TM, Meglin BM, Bird IM, Magness RR, 1998. Endothelial vasodilator production by uterine and systemic arteries. III. Ovarian and estrogen effects on NO synthase. Am J Physiol, 275, 1845-1856.
  • Weiner CR, Lizasoain I, Baylis SA, Knowles RG, Charles IG, Moncada S, 1994. Induction of calcium-dependent nitric oxide synthases by sex hormones. Proc Natl Acad Sci USA, 91, 5212- 5216.
  • Yallampalli C, Dong YL, 2000. Estradiol-17b inhibits nitric oxide synthase (NOS)-II and stimulates NOS-III gene expression in the rat uterus. Biol Reprod, 63, 34-41.
  • Yallampalli C, Dong YL, Gangula PR, Fong L, 1998. Role and regulation of nitric oxide in the uterus during pregnancy and parturition. J Soc Gynecol Invest, 5, 58-67.
  • Yilmaz O, Calka J, Bukowski R, Zalecki M, Wasowicz K, Jaroszewski JJ, Markiewicz W, Bulbul A, Ucar M, 2012. Nitric oxide in the bovine oviduct: Influence on contractile activity and nitric oxide synthase isoforms localization. Theriogenology, 77, 1312-1327.
  • Zhang J, Massmann GA, Mirabile CP, Figueroa JP, 1999. Non-pregnant sheep uterine type I and type III nitric oxide synthase expression is differentially regulated by estrogen. Biol Reprod, 60, 1198-1203
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Diğer ID JA69GH44EZ
Bölüm Araştırma
Yazarlar

Oktay Yilmaz Bu kişi benim

Artay Yagci Bu kişi benim

Elmas Ulutas Bu kişi benim

Alper Sevimli Bu kişi benim

Korhan Altunbas Bu kişi benim

Aziz Bulbul Bu kişi benim

Recep Aslan Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 29 Sayı: 2

Kaynak Göster

APA Yilmaz, O., Yagci, A., Ulutas, E., Sevimli, A., vd. (2013). The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus. Eurasian Journal of Veterinary Sciences, 29(2), 65-69.
AMA Yilmaz O, Yagci A, Ulutas E, Sevimli A, Altunbas K, Bulbul A, Aslan R. The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus. Eurasian J Vet Sci. Haziran 2013;29(2):65-69.
Chicago Yilmaz, Oktay, Artay Yagci, Elmas Ulutas, Alper Sevimli, Korhan Altunbas, Aziz Bulbul, ve Recep Aslan. “The Effect of 17β Estradiol on the Expression of ENOS and INOS in Ovariectomized Rat Uterus”. Eurasian Journal of Veterinary Sciences 29, sy. 2 (Haziran 2013): 65-69.
EndNote Yilmaz O, Yagci A, Ulutas E, Sevimli A, Altunbas K, Bulbul A, Aslan R (01 Haziran 2013) The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus. Eurasian Journal of Veterinary Sciences 29 2 65–69.
IEEE O. Yilmaz, A. Yagci, E. Ulutas, A. Sevimli, K. Altunbas, A. Bulbul, ve R. Aslan, “The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus”, Eurasian J Vet Sci, c. 29, sy. 2, ss. 65–69, 2013.
ISNAD Yilmaz, Oktay vd. “The Effect of 17β Estradiol on the Expression of ENOS and INOS in Ovariectomized Rat Uterus”. Eurasian Journal of Veterinary Sciences 29/2 (Haziran 2013), 65-69.
JAMA Yilmaz O, Yagci A, Ulutas E, Sevimli A, Altunbas K, Bulbul A, Aslan R. The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus. Eurasian J Vet Sci. 2013;29:65–69.
MLA Yilmaz, Oktay vd. “The Effect of 17β Estradiol on the Expression of ENOS and INOS in Ovariectomized Rat Uterus”. Eurasian Journal of Veterinary Sciences, c. 29, sy. 2, 2013, ss. 65-69.
Vancouver Yilmaz O, Yagci A, Ulutas E, Sevimli A, Altunbas K, Bulbul A, Aslan R. The effect of 17β estradiol on the expression of eNOS and iNOS in ovariectomized rat uterus. Eurasian J Vet Sci. 2013;29(2):65-9.