Our hypothesis was to develop a timed-AI protocol with good fertility but reduced cost and labour under commercial conditions.Heifers were divided two group and first group (n=19) received two injections of PGF2a 14 days apart. GnRH was injected 48 h afterthe second PGF2a injection and heifers were inseminated 14-16 h following GnRH (day 0; PPG group). This synchronization regimenwas compared with a widely used progesterone-based protocol.In the implant group (IMP group; n=29), a norgestomet ear implant was used and estradiol valerate plus norgestomet injected (i.m.;day 0). On Day 8, heifers received PGF2a and implant was removed on day 9. Heifers were inseminated 48 h after implant removal.Pregnancy diagnosis was performed by real-time ultrasound between day 31 and 38. Eleven of 19 heifers in the PPG group werepregnant (57.9%) compared to 14 of 29 heifers in the IMP group (48.2%; P>0.05 Chi-squared) Thus, the timed-AI protocol utilizing twodoses of PGF2a and GnRH resulted in acceptable pregnancy rates compared to the progesterone-based protocol which was morelabour intensive
Bu çalýþmanýn amacý, saha þartlarýnda maliyet ve iþ gücünü azaltan ancak fertilite oraný yüksek bir sabit zamanlý tohumlamaprotokolü geliþtirmektir. Daha önce geliþtirdiðimiz modifiye ovsynch senkronizasyon yöntemi, yaygýn olarak uygulanan progesteron esaslýbir protokolle (kulak implantý) karþýlaþtýrýldý. Bu amaçla birinci grup 19 baþ düveye 14 gün arayla iki kez PGF2a enjeksiyonu yapýldý(PPG grubu). Ýkinci PGF2a enjeksiyonundan 48 saat sonra GnRH enjekte edildi ve GnRH enjeksiyonundan 14-16 saat sonra sabitzamanlý tohumlama yapýldý (0. gün). Ýkinci grup progesteron esaslý protokolde ise 29 baþ düveye önce norgestomet kulak implantý takýldý(IMP grubu), östradiol valerat ve norgestomet içeren enjektabl solusyon kas içi uygulandý (0. gün). Uygulamayý takiben 8. günde PGF2a uygulandý ve 9. günde implant çýkarýldý. Tohumlamalar implantýn çýkarýlmasýný izleyen 48. saatte yapýldý. Tohumlanan düvelerin gebelikmuayeneleri 31-38. günlerde real-time ultrason ile yapýldý. Elde edilen gebelik/gebe kalma oraný PPG grubunda % 57.9 (11/19), IMPgrubunda % 48.2 (14/29) olarak belirlendi ve istatistiki fark görülmedi. Sonuç olarak 14 gün arayla iki doz PGF2a ve GnRH içeren sabitzamanlý tohumlama protokolü, yoðun iþ gücü gerektiren progesteron esaslý protokolle karþýlaþtýrýldýðýnda kabul edilebilir düzeyde gebelikoraný elde edilmektedir
Other ID | JA69TR76UV |
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Journal Section | Research |
Authors | |
Publication Date | March 1, 2008 |
Published in Issue | Year 2008 Volume: 24 Issue: 1 |