Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen

M. Bozkurt Ataman; Cengiz Yıldız; Abdullah Kaya; Melih Aksoy; Necdet Lehimcioğlu

KÖPEKLERDE TAZE ve DONMUŞ-ÇÖZÜNMÜŞ SPERMADA ANORMAL SPERMATOZOON TİPLERİNİN ve ORANLARININ TESPİT EDİLMESİNDE FARKLI YÖNTEMLERİN KULLANILMASI

Year 1998, Volume: 14 Issue: 2, 121 - 129, 01.06.1998

M. Bozkurt Ataman Cengiz Yıldız Abdullah Kaya Melih Aksoy Necdet Lehimcioğlu

Abstract

Bu çalışmada, köpeklerde taze ve donmuş-çözünmüş spermada anormal spermatozoon oranı ve tiplerinin tespit edilmesinde larldı Hancock solusyonu, Eosin·nigrosin, Hematoksilen·eosin ve Caserett boyaları kuJlanılarak anormal spematozoon oran· lan belirlendi. Ejakülatın geri kalan kısmı Tris-Frukloz sulandırıcısı kullanılarak 0.25 ml'lik payeller içerisinde don· duruldu. Payeller çözdürüldüklen sonra taze spermada anormal spermatozoon oranını tespit etmek amacı ile yapılan tüm muayeneler aynı yöntemler kullanılarak tekrar edildi. Anormal spermaıozoon oranlarının tespiti için kullanılan yöntemler arasında Istatistiki lark gözlenmedi (p>0.05). Sonuç olarak; taze ve donmuş-çôzünmüş spermada anormal spermatozoon oranlannın belirlenebilmesi Için Hancock solusyonu yanında farklı boyama yönıemlerinin de başarıyla kullanılabileceği kanısına varıldı

Keywords

Köpek, anormal spermatozoon, boyama yöntemleri, taze ve çözünmüş sperma

Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen

Year 1998, Volume: 14 Issue: 2, 121 - 129, 01.06.1998

M. Bozkurt Ataman Cengiz Yıldız Abdullah Kaya Melih Aksoy Necdet Lehimcioğlu

Abstract

Ef1ectiveness of the various stalning methods to detect abnormal sperm types and rates in fresh and frozen·lhawed dog semen were studied. Semen samples were colleCled by digital manipulation. Abnormal sperm rates ollresh semen samples were deıermined lollowing Ihe fixaıion in Hanrock solution or in the smears stained by eosinnigrosin, hematoksilen-eosln and Caserette stains. Morphological examinalion ol the semen sanyles were also con· ducted lollowing Ireezing and thawing in Tris-lructose extender in 0.25 ml of French straw. Analyses ol lhe data shawed Ihat there is no slatistic difference (p>O.OS) among the fixation and stain groups to determine abnormal sperm types and rales of fresh or Irozen·thawed dog semen. As a conclusion; in addition lo Haneock solution. dilleren sla· ining methods can be used to delect morphologically abnormal sperm in Iresh and frozen-thawed dog semen

Keywords

Dog, abnormal spermatozoon, staining method, fresh and thawed semen

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Details

Other ID	JA73ET54KC
Journal Section	Research
Authors	M. Bozkurt Ataman This is me Cengiz Yıldız This is me Abdullah Kaya This is me Melih Aksoy This is me Necdet Lehimcioğlu This is me
Publication Date	June 1, 1998
Published in Issue	Year 1998 Volume: 14 Issue: 2

Cite

APA	Ataman, M. B., Yıldız, C., Kaya, A., Aksoy, M., et al. (1998). Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian Journal of Veterinary Sciences, 14(2), 121-129.
AMA	Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. June 1998;14(2):121-129.
Chicago	Ataman, M. Bozkurt, Cengiz Yıldız, Abdullah Kaya, Melih Aksoy, and Necdet Lehimcioğlu. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences 14, no. 2 (June 1998): 121-29.
EndNote	Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N (June 1, 1998) Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian Journal of Veterinary Sciences 14 2 121–129.
IEEE	M. B. Ataman, C. Yıldız, A. Kaya, M. Aksoy, and N. Lehimcioğlu, “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen”, Eurasian J Vet Sci, vol. 14, no. 2, pp. 121–129, 1998.
ISNAD	Ataman, M. Bozkurt et al. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences 14/2 (June 1998), 121-129.
JAMA	Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. 1998;14:121–129.
MLA	Ataman, M. Bozkurt et al. “Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-Thawed Dog Semen”. Eurasian Journal of Veterinary Sciences, vol. 14, no. 2, 1998, pp. 121-9.
Vancouver	Ataman MB, Yıldız C, Kaya A, Aksoy M, Lehimcioğlu N. Effectiveness of the Various Staining Methods to Detect Abnormal Sperm Types and Rates in Fresh and Frozen-thawed Dog Semen. Eurasian J Vet Sci. 1998;14(2):121-9.