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Thiol/disulphide homeostasis in Helicobacter pylori infected patients

Year 2019, Volume: 5 Issue: 6, 948 - 956, 04.11.2019
https://doi.org/10.18621/eurj.443557

Abstract

Objectives:
The aim of the
study was to evaluate the oxidative stress level in patients, diagnosed with
Helicobacter pylori infection, using a
novel marker (thiol/
disulphide homeostasis) and to compare the level in infected
individuals with that in healthy volunteers.

Methods: A total of 60 patients diagnosed with gastritis, erosive gastritis or ulcer by endoscopy were included and biopsied.
The
30 patients diagnosed with H. pylori and 30 healthy individuals were enrolled. Medical histories, physical examination results, body mass index (BMI), hemogram, free triiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating
hormone (TSH), urea, creatinin, aspartate aminotransferase (AST), alanine
aminotransferase (ALT), albumin, total cholesterol,
triglycerides, high density lipoprotein (HDL), low density lipoprotein) LDL and thiol/disulphide levels obtained in the study groups were
compared.

Results: There was no significant difference between
the total thiol
, native 
thiol,
di
sulphide/native thiol and dissulphide/total thiol ratios of the patient and
control group.
When the H. pylori patients were stratified by
endoscopic evaluation as having mild (superficial gastritis or normal
appearance) or severe (ulcer or erosive areas)

symptoms,
there were significant differences in disulphide, disulphide/native thiol, disulphide/total thiol and native thiol/total
thiol levels
. We also observed BMI and the total, native thiol levels of H. pylori patients were inversely
related.( r: 0.562,
p = 0.001; r: 0.0552,
p = 0.002).







Conclusions: Thiol/disulphide homeostasis is likely to differ with
both duration and severity of
H. pylori
infection. Further investigations are needed to investigate the effect of
H. pylori on oxidative stress.

References

  • [1] Williams MP, Pounder RE. Helicobacter pylori: from the benign to the malignant. Am J Gastroenterol 1999;94(11 Suppl):S11-6.
  • [2] Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 2006;19:449-90.
  • [3] Blaser MJ. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990;161:626-33.
  • [4] Zoorob RJ. NIH consensus on Helicobacter pylori in peptic ulcer disease. J Am Board Fam Pract 1996;9:392.
  • [5] He C, Yang Z, Lu NH. Helicobacter pylori-an infectious risk factor for atherosclerosis? J Atheroscler Thromb 2014;21:1229-42.
  • [6] Lai CY, Yang TY, Lin CL, Kao CH. Helicobacter pylori infection and the risk of acute coronary syndrome: a nationwide retrospective cohort study. Eur J Clin Microbiol Infect Dis 2015;34:69-74.
  • [7] Noonavath RN, Lakshmi CP, Dutta TK, Kate V. Helicobacter pylori eradication in patients with chronic immune thrombocytopenic purpura. World J Gastroenterol 2014;20:6918-23.
  • [8] Marietti M, Gasbarrini A, Saracco G, Pellicano R. Helicobacter pylori infection and diabetes mellitus: the 2013 state of art. Panminerva Med 2013;55:277-81.
  • [9] Ustundag Y, Huysal K, Kahvecioglu S, Demirci H, Yavuz S, Sambel M, et al. Establishing reference values and evaluation of an in-house ferric reducing antioxidant power (FRAP) colorimetric assay in microplates. Eur Res J 2016;2:126-31.
  • [10] Butcher LD, den Hartog G, Ernst PB, Crowe SE. Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis. Cell Mol Gastroenterol Hepatol 2017;3:316-22.
  • [11] Pignatelli B, Bancel B, Plummer M, Toyokuni S, Patricot LM, Ohshima H. Helicobacter pylori eradication attenuates oxidative stress in human gastric mucosa. Am J Gastroenterol 2001;96:1758-66.
  • [12] Baek HY, Lim JW, Kim H, Kim JM, Kim JS, Jung HC, et al. Oxidative-stress-related proteome changes in Helicobacter pylori-infected human gastric mucosa. Biochem J 2004;379(Pt 2):291-9.
  • [13] Lee WP, Hou MC, Lan KH, Li CP, Chao Y, Lin HC, et al. Helicobacter pylori-induced chronic inflammation causes telomere shortening of gastric mucosa by promoting PARP-1-mediated non-homologous end joining of DNA. Arch Biochem Biophys 2016;606:90-8.
  • [14] Shirin H, Pinto JT, Liu LU, Merzianu M, Sordillo EM, Moss SF. Helicobacter pylori decreases gastric mucosal glutathione. Cancer Lett 2001;164:127-33.
  • [15] Ernst PB, Gold BD. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu Rev Microbiol 2000;54:615-40.
  • [16] Kuper H, Adami HO, Trichopoulos D. Infections as a major preventable cause of human cancer. J Intern Med 2000;248:171-83.
  • [17] Handa O, Naito Y, Yoshikawa T. Helicobacter pylori: a ROS-inducing bacterial species in the stomach. Inflamm Res 2010;59:997-1003.
  • [18] Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000;72(2 Suppl):653S-69S.
  • [19] Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med 2013;65:244-53.
  • [20] Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009;47:1329-38.
  • [21] Eroğlu O, Dindar Badem N, Baccıoğlu A, Cömertpay E, Neşelioğlu S, Erel Ö. Significance of thiol/disulphide homeostasis and ischemia modified albumin levels in chronic obstructive pulmonary disease. Eur Res J 2019;5:250-7.
  • [22] Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32.
  • [23] Naja F, Kreiger N, McKeown Eyssen G, Allard J. Bioavailability of vitamins E and C: does Helicobacter pylori infection play a role? Ann Nutr Metab 2010;56:253-9.
  • [24] Roesler BM, Rabelo-Gonçalves EM, Zeitune JM. Virulence factors of Helicobacter pylori: a review. Clin Med Insights Gastroenterol 2014;7:9-17.
  • [25] Gong M, Ling SS, Lui SY, Yeoh KG, Ho B. Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease. Gastroenterology 2010;139:564-73.
  • [26] Chaturvedi R, Asim M, Romero-Gallo J, Barry DP, Hoge S, de Sablet T, et al. Spermine oxidase mediates the gastric cancer risk associated with Helicobacter pylori CagA. Gastroenterology 2011;141:1696-708 e1-2.
  • [27] Wang G, Hong Y, Olczak A, Maier SE, Maier RJ. Dual roles of Helicobacter pylori NapA in inducing and combating oxidative stress. Infect Immun 2006;74:6839-46.
  • [28] Yanaka A. Role of NRF2 in protection of the gastrointestinal tract against oxidative stress. J Clin Biochem Nutr 2018;63:18-25.
  • [29] Elmas B, Karacan M, Dervişoğlu P, Kösecik M, İşgüven ŞP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol 2017;18:361-9.
  • [30] Söğüt İ, Şenat Aydın A, Sağlam Gökmen E, Gün Atak P, Erel Ö, Görmüş DeGrigo U. Evaluation of oxidative stress and thiol-disulfide parameters according to the body mass index in adult individuals. Erciyes Med J 2018;40:155-6.
  • [31] Polat OA, Kurt A, Kılıç R, Nar R, Kocamış Ö. Is there any association between the retinal vein occlusion and the thiol-disulfide homeostasis which is an oxidative stress indicator? Turkiye Klinikleri J Ophthalmol 2019;28:23-8.
  • [32] Ramos LF, Shintani A, Ikizler TA, Himmelfarb J. Oxidative stress and inflammation are associated with adiposity in moderate to severe chronic kidney disease. J Am Soc Nephrol 2008;19:593-9.
  • [33] Fidan F, Alkan BM, Uğurlu FG, Bozkurt S, Sezer N, Biçer C, et al. Dynamic thiol/disulfide homeostasis in patients with fibromyalgia. Arch Rheumatol 2017;32:112-7.
  • [34] Şimşek Ö, Çarlıoğlu A, Alışık M, Edem E, Biçer CK. Thiol/disulfide balance in patients with familial hypercholesterolemia. Cardiol Res Pract 2018;2018:9042461.
  • [35] Parlak ES, Alisik M, Karalezli A, Sayilir AG, Bastug S, Er M. Are the thiol/disulfide redox status and HDL cholesterol levels associated with pulmonary embolism? Thiol/disulfide redox status in pulmonary embolism. Clin Biochem 2017;50:1020-4.
  • [36] Üstündağ Budak Y, Kahvecioğlu S, Çelik H, Alışık M, Erel Ö. Serum thiol/disulfide homeostasis in hemodialysis, peritoneal dialysis, and renal transplantation patients. Turk Neph Dial Transpl 2017;26:105-10.
Year 2019, Volume: 5 Issue: 6, 948 - 956, 04.11.2019
https://doi.org/10.18621/eurj.443557

Abstract

References

  • [1] Williams MP, Pounder RE. Helicobacter pylori: from the benign to the malignant. Am J Gastroenterol 1999;94(11 Suppl):S11-6.
  • [2] Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev 2006;19:449-90.
  • [3] Blaser MJ. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990;161:626-33.
  • [4] Zoorob RJ. NIH consensus on Helicobacter pylori in peptic ulcer disease. J Am Board Fam Pract 1996;9:392.
  • [5] He C, Yang Z, Lu NH. Helicobacter pylori-an infectious risk factor for atherosclerosis? J Atheroscler Thromb 2014;21:1229-42.
  • [6] Lai CY, Yang TY, Lin CL, Kao CH. Helicobacter pylori infection and the risk of acute coronary syndrome: a nationwide retrospective cohort study. Eur J Clin Microbiol Infect Dis 2015;34:69-74.
  • [7] Noonavath RN, Lakshmi CP, Dutta TK, Kate V. Helicobacter pylori eradication in patients with chronic immune thrombocytopenic purpura. World J Gastroenterol 2014;20:6918-23.
  • [8] Marietti M, Gasbarrini A, Saracco G, Pellicano R. Helicobacter pylori infection and diabetes mellitus: the 2013 state of art. Panminerva Med 2013;55:277-81.
  • [9] Ustundag Y, Huysal K, Kahvecioglu S, Demirci H, Yavuz S, Sambel M, et al. Establishing reference values and evaluation of an in-house ferric reducing antioxidant power (FRAP) colorimetric assay in microplates. Eur Res J 2016;2:126-31.
  • [10] Butcher LD, den Hartog G, Ernst PB, Crowe SE. Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis. Cell Mol Gastroenterol Hepatol 2017;3:316-22.
  • [11] Pignatelli B, Bancel B, Plummer M, Toyokuni S, Patricot LM, Ohshima H. Helicobacter pylori eradication attenuates oxidative stress in human gastric mucosa. Am J Gastroenterol 2001;96:1758-66.
  • [12] Baek HY, Lim JW, Kim H, Kim JM, Kim JS, Jung HC, et al. Oxidative-stress-related proteome changes in Helicobacter pylori-infected human gastric mucosa. Biochem J 2004;379(Pt 2):291-9.
  • [13] Lee WP, Hou MC, Lan KH, Li CP, Chao Y, Lin HC, et al. Helicobacter pylori-induced chronic inflammation causes telomere shortening of gastric mucosa by promoting PARP-1-mediated non-homologous end joining of DNA. Arch Biochem Biophys 2016;606:90-8.
  • [14] Shirin H, Pinto JT, Liu LU, Merzianu M, Sordillo EM, Moss SF. Helicobacter pylori decreases gastric mucosal glutathione. Cancer Lett 2001;164:127-33.
  • [15] Ernst PB, Gold BD. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu Rev Microbiol 2000;54:615-40.
  • [16] Kuper H, Adami HO, Trichopoulos D. Infections as a major preventable cause of human cancer. J Intern Med 2000;248:171-83.
  • [17] Handa O, Naito Y, Yoshikawa T. Helicobacter pylori: a ROS-inducing bacterial species in the stomach. Inflamm Res 2010;59:997-1003.
  • [18] Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000;72(2 Suppl):653S-69S.
  • [19] Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med 2013;65:244-53.
  • [20] Jones DP, Liang Y. Measuring the poise of thiol/disulfide couples in vivo. Free Radic Biol Med 2009;47:1329-38.
  • [21] Eroğlu O, Dindar Badem N, Baccıoğlu A, Cömertpay E, Neşelioğlu S, Erel Ö. Significance of thiol/disulphide homeostasis and ischemia modified albumin levels in chronic obstructive pulmonary disease. Eur Res J 2019;5:250-7.
  • [22] Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32.
  • [23] Naja F, Kreiger N, McKeown Eyssen G, Allard J. Bioavailability of vitamins E and C: does Helicobacter pylori infection play a role? Ann Nutr Metab 2010;56:253-9.
  • [24] Roesler BM, Rabelo-Gonçalves EM, Zeitune JM. Virulence factors of Helicobacter pylori: a review. Clin Med Insights Gastroenterol 2014;7:9-17.
  • [25] Gong M, Ling SS, Lui SY, Yeoh KG, Ho B. Helicobacter pylori gamma-glutamyl transpeptidase is a pathogenic factor in the development of peptic ulcer disease. Gastroenterology 2010;139:564-73.
  • [26] Chaturvedi R, Asim M, Romero-Gallo J, Barry DP, Hoge S, de Sablet T, et al. Spermine oxidase mediates the gastric cancer risk associated with Helicobacter pylori CagA. Gastroenterology 2011;141:1696-708 e1-2.
  • [27] Wang G, Hong Y, Olczak A, Maier SE, Maier RJ. Dual roles of Helicobacter pylori NapA in inducing and combating oxidative stress. Infect Immun 2006;74:6839-46.
  • [28] Yanaka A. Role of NRF2 in protection of the gastrointestinal tract against oxidative stress. J Clin Biochem Nutr 2018;63:18-25.
  • [29] Elmas B, Karacan M, Dervişoğlu P, Kösecik M, İşgüven ŞP, Bal C. Dynamic thiol/disulphide homeostasis as a novel indicator of oxidative stress in obese children and its relationship with inflammatory-cardiovascular markers. Anatol J Cardiol 2017;18:361-9.
  • [30] Söğüt İ, Şenat Aydın A, Sağlam Gökmen E, Gün Atak P, Erel Ö, Görmüş DeGrigo U. Evaluation of oxidative stress and thiol-disulfide parameters according to the body mass index in adult individuals. Erciyes Med J 2018;40:155-6.
  • [31] Polat OA, Kurt A, Kılıç R, Nar R, Kocamış Ö. Is there any association between the retinal vein occlusion and the thiol-disulfide homeostasis which is an oxidative stress indicator? Turkiye Klinikleri J Ophthalmol 2019;28:23-8.
  • [32] Ramos LF, Shintani A, Ikizler TA, Himmelfarb J. Oxidative stress and inflammation are associated with adiposity in moderate to severe chronic kidney disease. J Am Soc Nephrol 2008;19:593-9.
  • [33] Fidan F, Alkan BM, Uğurlu FG, Bozkurt S, Sezer N, Biçer C, et al. Dynamic thiol/disulfide homeostasis in patients with fibromyalgia. Arch Rheumatol 2017;32:112-7.
  • [34] Şimşek Ö, Çarlıoğlu A, Alışık M, Edem E, Biçer CK. Thiol/disulfide balance in patients with familial hypercholesterolemia. Cardiol Res Pract 2018;2018:9042461.
  • [35] Parlak ES, Alisik M, Karalezli A, Sayilir AG, Bastug S, Er M. Are the thiol/disulfide redox status and HDL cholesterol levels associated with pulmonary embolism? Thiol/disulfide redox status in pulmonary embolism. Clin Biochem 2017;50:1020-4.
  • [36] Üstündağ Budak Y, Kahvecioğlu S, Çelik H, Alışık M, Erel Ö. Serum thiol/disulfide homeostasis in hemodialysis, peritoneal dialysis, and renal transplantation patients. Turk Neph Dial Transpl 2017;26:105-10.
There are 36 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other), Endocrinology, Gastroenterology and Hepatology, ​Internal Diseases
Journal Section Original Articles
Authors

Ahmed Ramiz Baykan 0000-0001-6798-0240

Cemile Biçer This is me 0000-0001-7937-4475

Emre Gerçeker This is me 0000-0001-7494-2912

Özcan Erel 0000-0002-2996-3236

Serkan Cerrah This is me 0000-0002-9139-8039

Bülent Albayrak This is me 0000-0003-1231-152X

Mustafa Utlu 0000-0002-6148-6644

Ayşe Kargılı This is me 0000-0002-5622-9563

Publication Date November 4, 2019
Submission Date July 13, 2018
Acceptance Date January 23, 2019
Published in Issue Year 2019 Volume: 5 Issue: 6

Cite

AMA Baykan AR, Biçer C, Gerçeker E, Erel Ö, Cerrah S, Albayrak B, Utlu M, Kargılı A. Thiol/disulphide homeostasis in Helicobacter pylori infected patients. Eur Res J. November 2019;5(6):948-956. doi:10.18621/eurj.443557

e-ISSN: 2149-3189 


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