The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey
Öz
A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected
during one year period (2014-16) from three cities (Sivas, Malatya and Elazığ) located central Turkey. 432 bp partial
sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank.
Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed.
As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves
from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted
under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus
which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was
substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from
51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection
and phylogenetic analysis of Bovine astrovirus from Turkey.
Anahtar Kelimeler
Kaynakça
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Ayrıntılar
Birincil Dil
İngilizce
Konular
-
Bölüm
Araştırma Makalesi
Yayımlanma Tarihi
30 Aralık 2018
Gönderilme Tarihi
15 Temmuz 2018
Kabul Tarihi
7 Ağustos 2018
Yayımlandığı Sayı
Yıl 2018 Cilt: 29 Sayı: 2
APA
Turan, T., & Işıdan, H. (2018). The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Veteriner Mikrobiyoloji Dergisi, 29(2), 104-110. https://doi.org/10.35864/evmd.513442
AMA
1.Turan T, Işıdan H. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 2018;29(2):104-110. doi:10.35864/evmd.513442
Chicago
Turan, Turhan, ve Hakan Işıdan. 2018. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi 29 (2): 104-10. https://doi.org/10.35864/evmd.513442.
EndNote
Turan T, Işıdan H (01 Aralık 2018) The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Veteriner Mikrobiyoloji Dergisi 29 2 104–110.
IEEE
[1]T. Turan ve H. Işıdan, “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”, Etlik Vet. Mik. Derg., c. 29, sy 2, ss. 104–110, Ara. 2018, doi: 10.35864/evmd.513442.
ISNAD
Turan, Turhan - Işıdan, Hakan. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi 29/2 (01 Aralık 2018): 104-110. https://doi.org/10.35864/evmd.513442.
JAMA
1.Turan T, Işıdan H. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 2018;29:104–110.
MLA
Turan, Turhan, ve Hakan Işıdan. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi, c. 29, sy 2, Aralık 2018, ss. 104-10, doi:10.35864/evmd.513442.
Vancouver
1.Turhan Turan, Hakan Işıdan. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 01 Aralık 2018;29(2):104-10. doi:10.35864/evmd.513442
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