Araştırma Makalesi

The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

Cilt: 29 Sayı: 2 30 Aralık 2018
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The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

Öz

A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected
during one year period (2014-16) from three cities (Sivas, Malatya and Elazığ) located central Turkey. 432 bp partial
sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank.
Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed.
As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves
from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted
under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus
which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was
substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from
51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection
and phylogenetic analysis of Bovine astrovirus from Turkey.

Anahtar Kelimeler

Kaynakça

  1. 1. Alfred N, Liu H, Li ML, Hong SF, Tang HB, Wei ZZ, Chen Y, Li FK, Zhong YZ, Huang WJ, (2015). Molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in China. J Vet Med Sci, 77, 643–651. DOI:10.1292/jvms.14-0252
  2. 2. Atkins A, Wellehan JF Jr, Childress AL, Archer LL, Fraser WA, Citino SB, (2009). Characterization of an outbreak of astroviral diarrhea in a group of cheetahs (Acinonyx jubatus). Vet Microbiol, 136, 160–165, 2009. DOI:10.1016/j.vetmic.2008.10.035
  3. 3. Bartels CJM, Holzhauer M, Jorritsma R, et al (2010) Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Prev Vet Med 93:162–169. doi: 10.1016/j. prevetmed.2009.09.020
  4. 4. Benedictis PD, Schultz-Cherry S, Burnham A, Cattoli G, (2011). Astrovirus infections in humans and animals – Molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol, 11, 1529–1544. DOI:10.1016/j.meegid.2011.07.024
  5. 5. Bridger JC, Hall GA, Brown JF, (1984). Characterization of a calici-like virus (Newbury agent) found in association with astrovirus in bovine diarrhea. Infect Immun, 43, 133–138.
  6. 6. Candido M, Alencar ALF, Almeida-Queiroz SR, Buzinaro MG, Munin FS, de Godoy SHS, Livonesi MC, Fernandes AM, de Sousa RLM, (2015). Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil. Arch Virol, 160, 1519–1525. DOI: 10.1007/ s00705-015-2400-8
  7. 7. Chen X, Zhang B, Yue H, Wang Y, Zhou F, Zhang Q, Tang C, (2015). A novel astrovirus species in the gut of yaks with diarrhoea in the Qinghai–Tibetan Plateau, 2013. J Gen Virol, 96, 3672–3680. DOI:10.1099/jgv.0.000303
  8. 8. Cho Y il, Yoon KJ (2014) An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. J Vet Sci 15:1–17. doi: 10.4142/jvs.2014.15.1.1

Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yayımlanma Tarihi

30 Aralık 2018

Gönderilme Tarihi

15 Temmuz 2018

Kabul Tarihi

7 Ağustos 2018

Yayımlandığı Sayı

Yıl 2018 Cilt: 29 Sayı: 2

Kaynak Göster

APA
Turan, T., & Işıdan, H. (2018). The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Veteriner Mikrobiyoloji Dergisi, 29(2), 104-110. https://doi.org/10.35864/evmd.513442
AMA
1.Turan T, Işıdan H. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 2018;29(2):104-110. doi:10.35864/evmd.513442
Chicago
Turan, Turhan, ve Hakan Işıdan. 2018. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi 29 (2): 104-10. https://doi.org/10.35864/evmd.513442.
EndNote
Turan T, Işıdan H (01 Aralık 2018) The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Veteriner Mikrobiyoloji Dergisi 29 2 104–110.
IEEE
[1]T. Turan ve H. Işıdan, “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”, Etlik Vet. Mik. Derg., c. 29, sy 2, ss. 104–110, Ara. 2018, doi: 10.35864/evmd.513442.
ISNAD
Turan, Turhan - Işıdan, Hakan. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi 29/2 (01 Aralık 2018): 104-110. https://doi.org/10.35864/evmd.513442.
JAMA
1.Turan T, Işıdan H. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 2018;29:104–110.
MLA
Turan, Turhan, ve Hakan Işıdan. “The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey”. Etlik Veteriner Mikrobiyoloji Dergisi, c. 29, sy 2, Aralık 2018, ss. 104-10, doi:10.35864/evmd.513442.
Vancouver
1.Turhan Turan, Hakan Işıdan. The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey. Etlik Vet. Mik. Derg. 01 Aralık 2018;29(2):104-10. doi:10.35864/evmd.513442

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