The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

Yıl 2018, , 104 - 110, 30.12.2018

Turhan Turan Hakan Işıdan

https://doi.org/10.35864/evmd.513442

Cited By: 4

Öz

A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected

during one year period (2014-16) from three cities (Sivas, Malatya and Elazığ) located central Turkey. 432 bp partial

sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank.

Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed.

As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves

from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted

under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus

which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was

substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from

51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection

and phylogenetic analysis of Bovine astrovirus from Turkey.

Anahtar Kelimeler

Bovine astrovirus, calf, phylogenetic analysis, Turkey

Kaynakça

• 1. Alfred N, Liu H, Li ML, Hong SF, Tang HB, Wei ZZ, Chen Y, Li FK, Zhong YZ, Huang WJ, (2015). Molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in China. J Vet Med Sci, 77, 643–651. DOI:10.1292/jvms.14-0252
• 2. Atkins A, Wellehan JF Jr, Childress AL, Archer LL, Fraser WA, Citino SB, (2009). Characterization of an outbreak of astroviral diarrhea in a group of cheetahs (Acinonyx jubatus). Vet Microbiol, 136, 160–165, 2009. DOI:10.1016/j.vetmic.2008.10.035
• 3. Bartels CJM, Holzhauer M, Jorritsma R, et al (2010) Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Prev Vet Med 93:162–169. doi: 10.1016/j. prevetmed.2009.09.020
• 4. Benedictis PD, Schultz-Cherry S, Burnham A, Cattoli G, (2011). Astrovirus infections in humans and animals – Molecular biology, genetic diversity, and interspecies transmissions. Infect Genet Evol, 11, 1529–1544. DOI:10.1016/j.meegid.2011.07.024
• 5. Bridger JC, Hall GA, Brown JF, (1984). Characterization of a calici-like virus (Newbury agent) found in association with astrovirus in bovine diarrhea. Infect Immun, 43, 133–138.
• 6. Candido M, Alencar ALF, Almeida-Queiroz SR, Buzinaro MG, Munin FS, de Godoy SHS, Livonesi MC, Fernandes AM, de Sousa RLM, (2015). Molecular detection and phylogenetic analysis of bovine astrovirus in Brazil. Arch Virol, 160, 1519–1525. DOI: 10.1007/ s00705-015-2400-8
• 7. Chen X, Zhang B, Yue H, Wang Y, Zhou F, Zhang Q, Tang C, (2015). A novel astrovirus species in the gut of yaks with diarrhoea in the Qinghai–Tibetan Plateau, 2013. J Gen Virol, 96, 3672–3680. DOI:10.1099/jgv.0.000303
• 8. Cho Y il, Yoon KJ (2014) An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. J Vet Sci 15:1–17. doi: 10.4142/jvs.2014.15.1.1
• 9. Chu DK, Chin AW, Smith GJ, Chan KH, Guan Y, Peiris JS, Poon LL, (2010). Detection of novel astroviruses in urban brown rats and previously known astroviruses in humans. J Gen Virol, 91, 2457–2462. DOI:10.1099/vir.0.022764-0
• 10. Jonassen CM, Jonassen TO, Saif YM, Snodgrass DR, Ushijima H, Shimizu M, Grinde B, (2001). Comparison of capsid sequences from human and animal astroviruses. J Gen Virol, 82, 1061–1067. DOI:10.1099/0022-1317-82- 5-1061
• 11. Li L, Diab S, Mc Graw S, Barr B, Traslavina R, Higgins R, Talbot T, Blanchard P, Rimoldi G, Fahsbender E, Page B, Phan TG, Wang C, Deng X, Pesavento P, Delwart E, (2013). Divergent astrovirus associated with neurologic disease in cattle. Emerging Infect Dis, 19, 1385-1392. DOI: 10.3201/eid1909.130682
• 12. Mendez E, Arias CF, (2007). Astroviruses. Knipe DM, Howley PM eds. Fields virology. Lippincott Williams & Wilkins, Philadelphia, USA, p.981-1000.
• 13. Monroe SS, (2005). Astroviridae. Carter MJ, Herrmann J, Mitchel JK Sanchez-Fauquier A eds. Virus taxonomy. Eighth report of the International Committee on Taxonomy of Viruses.Elsevier, Amsterdam, The Netherlands. P.859- 864.
• 14. Mohamed FF, Mansour FMG, El-Araby IE, Mor SK, Goyal SM, (2017). Molecular detection of enteric viruses from diarrheic calves in Egypt. Arch Virol, 162, 129–137. DOI: 10.1007/s00705-016-3088-0
• 15. Nagai M, Omatsu T, Aoki H, Otomaru K, Uto T, Koizumi M, Minami-Fukuda F, Takai H, Murakami T, Masuda T, Yamasato H, Shiokawa M, Tsuchiaka S, Naoi Y, Sano K, Okazaki S, Katayama Y, Oba M, Furuya T, Shirai J, Mizutani T, (2015). Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: identification of possible interspecies transmission of bovine astrovirus. Arch Virol, 160, 2491–2501. DOI: 10.1007/ s00705-015-2543-7
• 16. Okonechnikov K, Golosova O, Fursov M, and UGENE Team: Unipro UGENE, (2012). A unified bioinformatics toolkit. Bioinformatics, 28, 1166–1167. DOI: 10.1093/ bioinformatics/bts091
• 17. Pham NTK, Khamrin P, Nguyen TA, et al (2007) Isolation and Molecular Characterization of Aichi Viruses from Fecal Specimens Collected in Japan, Bangladesh, Thailand, and Vietnam. J Clin Microbiol 45:2287–2288. doi: 10.1128/JCM.00525-07
• 18. Reuter G, Boros Á, Pankovics P (2011) Kobuviruses - a comprehensive review. Rev Med Virol 21:32–41. doi: 10.1002/rmv.677
• 19. Rivera R, Nollens HH, Venn-Watson S, Gulland FM, Wellehan JF Jr, (2010). Characterization of phylogenetically diverse astroviruses of marine mammals. J Gen Virol, 91, 166–173. DOI:10.1099/vir.0.015222-0
• 20. Schlotta K, Schulze C, Bilk S, Hanke D, Hoeper D, Beer M, Hoffmann B, (2016). Detection of a Novel Bovine Astrovirus in a Cow with Encephalitis. Transbound Emerg Dis, 63, 253–259. DOI:10.1111/tbed.12493
• 21. Selimovic-Hamza S, Bouzalas IG, Vandevelde M, Oevermann A, and Seuberlich T, (2016). Detection of astrovirus in historical cases of european sporadic Bovine encephalitis, switzerland 1958–1976. Front Vet Sci, 3, 91. DOI:10.3389/fvets.2016.00091
• 22. Sharp CP, Gregory WF, Mason C, deC Bronsvoort BM, Beard PM, (2015). High prevalence and diversity of bovine astroviruses in the faeces of healthy and diarrhoeic calves in South West Scotland. Vet Microbiol, 178, 70–76. DOI:10.1016/j.vetmic.2015.05.002
• 23. Smits SL, Van LM, Kuiken T, Hammer AS, Simon JH, Osterhaus AD, (2010). Identification and characterization of deer astroviruses. J Gen Virol, 91, 2719–2722. DOI: 10.1099/vir. 0.024067-0
• 24. Snodgrass DR, Gray EW, (1977). Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Arch Virol, 55, 287–291.
• 25. Tse H, Chan WM, Tsoi HW, Fan RY, Lau CC, Lau SK, Woo PC, Yuen KY, (2011). Rediscovery and genomic characterization of bovine astroviruses. J Gen Virol 92, 1888–1898. DOI:10.1099/vir.0.030817-0
• 26. Turan T, Işıdan H, Atasoy MO, İrehan B, (2018). Detection and molecular analysis of bovine enteric norovirus and nebovirus in Turkey. J Vet Res 62, 129-135. DOI:10.2478/ jvetres-2018-0021
• 27. Woode GN, Bridger JC, (1978). Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol, 11, 441– 452. DOI: 10.1099/00222615-11-4-441
• 28. Woode GN, Pohlenz JF, Gourley NE, Fagerland JA, (1984). Astrovirus and Breda virus infections of dome cell epithelium of bovine ileum. J Clin Microbiol, 19: 623– 630. DOI: 0095-1137/84/050623-08$02.00/0